| 光谱学与光谱分析 |
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| A Spectral Analysis Method for Total Volatile Base Nitrogen |
| YAO Yan1,ZHANG Ping2*, CHEN Yong-heng2, TIAN Chang-en1, LIU Wen-feng2,WANG Zhen-chun1, SHEN Shui-bao1 |
1. Schools of Life Science, Guangzhou University, Guangzhou 510006, China
2. School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China |
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Abstract A new method for the determination of the total volatile basic nitrogen (TVB-N) by reflectance spectroscopy was developed. The method was based on the reaction of TVB-N with Nessler’s reagent treated by molecular sieve and the reflectance-absorption value F(R), which is directly proportional to the amount of NH2Hg2IO in solid phase, was measured by the reflection spectrometer. The fundamental principle, effective factors and experimental conditions of the method were discussed. The special features of this method were its simplicity in operation, relatively high sensitivity of determination and the use of a small amount of reagent. Linear calibration graph was obtained in the range 1-8 μg·mL-1 with a detection limit of 0.1 μg·mL-1. The method was applied to monitor the fresh degree of fish and pork. The result discovered that the total volatile basic nitrogen increased rapidly along with the time extension, indicating that the deterioration of fish and pork was an acceleration process, and the deterioration speed of fish was faster than that of pork.
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Received: 2008-05-12
Accepted: 2008-08-18
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Corresponding Authors:
ZHANG Ping
E-mail: zhangping@163.com
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[1] Pedro Castro, Juan Carlos Penedo Padrón, Ma José Caballero Cansino, et al. Food Control, 2006, 17(4): 245.
[2] WANG Shu-chun(王叔淳). Food Hygiene Check Technique Handbook(食品卫生检验技术手册). Beijing: Chemical Industry Press(北京: 化学工业出版社)，1994.
[3] CHEN Pei-ji, LI Lai-hao, LI Liu-dong, et al(陈培基, 李来好, 李刘冬, 等). Journal of Fishery Sciences of China(中国水产科学). 2006, 13(1): 146.
[4] YU Jian-liang, LI Kai-xiong, LU Shi-ling, et al(于见亮, 李开雄, 卢士玲, 等). Food Research and Development(食品研究与开发)，2008, 29(2): 141.
[5] WANG Dan-hong, CHEN Xiang-ming(王丹红, 陈祥明). Fujian Analysis & Testing(福建分析测试)，2007, 16(2): 84.
[6] DENG Hui-ping，LIN Kai, ZHANG Hong-yu, et al(邓辉萍, 林 凯, 张红宇, 等). Occupation and Health(职业与健康), 2005, 21(6): 838.
[7] FAN Hua-feng, LIU Zhen-lin(范华锋, 刘振林). Chinses Journal of Health Laboratory Technology(中国卫生检验杂志), 2002, 12(01): 83.
[8] Anissa Dhaouadi, Lotfi Monser, Saloua Sadok, et al. Food Chemistry, 2007, 103(3): 1049.
[9] Alexis Pacquit, King Tong Lau, Henry McLaughlin, et al. Talanta, 2006, 69(2): 515.
[10] HOU Rui-feng, HUANG Lan, WANG Zhong-yi, et al(侯瑞锋, 黄 岚, 王忠义, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006, 26(12): 2193.
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