光谱学与光谱分析 |
|
|
|
|
|
FTIR Spectrum and Coagulation Enhancement of Exopolysaccharide Secreted by an Antarctica Bacterium Pseudoalteromona sp. Bsi20310 |
ZHOU Wei-zhi1,2, SHEN Bo-ling1, LIU Sheng-bo2, CHEN Bo3, ZHANG Yu-zhong2 |
1. School of Environmental Science and Engineering, Shandong University, Jin’an 250100, China 2. State Key Lab of Microbial Technology, Shandong University, Ji’nan 250100, China 3. State Oceanic Adminstration People’s Republic of China Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China |
|
|
Abstract Bsi20310 exopolysaccharide (Bsi20310 EPS) was secreted by a bacteria named Pseudoalteromonas sp. Bsi20310, isolated from Antarctic Sea ice. Crude Bsi20310 EPS was prepared by precipitation of the culture solution with ethanol, with proteins removed by using chloroform and butanol preparatorily. The results showed that Bsi20310 EPS improved the FeCl3 coagulation performance on synthetic water-soluble dye reactive red X-3B dyeing wastewater, obviously. The optimum coagulation enhancement of Bsi20310 EPS expressed by decolorization rate is from 16% to 84%, at pH near 10, Fe(Ⅲ) concentration of 0.98 mmol·L-1 and Bsi20310 EPS concentration of 150 mg·L-1, respectively. Fourier transform infrared spectroscopy (FTIR) was used to investigate the functional groups of Bsi20310 EPS, Fe(Ⅲ)-Bsi20310 EPS floc and Fe(Ⅲ)-Bsi20310 EPS-reactive red X-3B floc. The spectra showed that Bsi20310 EPS contained a large number of functional groups such as —OH, —COOH and glycosidic bond. Some certain functional groups of Bsi20310 EPS changed being combined with Fe(Ⅲ) hydrolysate. For instance, narrow peaks at 3 429 and 1 650 cm-1 became wide; the peak at 2 921 cm-1 weakened or disappeared; the peak at 1 242 cm-1 red-shifted slightly; peaks in the region of 1 151-1 038 cm-1 became single and sharp, etc. The change in spectra indicated that —OH, —OOH and glycosidic bond might be the main functional groups. The study suggested a bright prospect of Bsi20310 EPS performing as an approach to safe and effective microbial coagulation enhancement.
|
Received: 2009-01-28
Accepted: 2009-05-02
|
|
Corresponding Authors:
ZHOU Wei-zhi
E-mail: wzzhou@sdu.edu.cn
|
|
[1] FANG Ming-hui, ZHAN Shu-lin, LIN Jun-xiong, et al(方明晖,詹树林,林俊雄,等). Industrial Water Treatment(工业水处理), 2007, 27(2): 26. [2] Kim T H, Park C, Yang J, et al. Journal of Hazardous Materials, 2004, 112(1-2): 95. [3] Aboulhassan M A, Souabi S, Yaacoubi A, et al. Journal of Hazardous Materials, 2006, 138(1,2): 40. [4] Renault F, Sancey B, Badot P M, et al. European Polymer Journal, 2009, 45(5): 1337. [5] LIU Zhong-wei, XIONG Rong-chun, WEI Gang(刘中卫,熊蓉春,魏 刚). Journal of Beijing University of Chemical Technology(北京化工大学学报), 2008, 35(6): 45. [6] Sanghi R, Bhattacharya B, Singh V. Reactive and Functional Polymers, 2007, 67 (6): 495. [7] LI Jiang, SONG Guo-qiang, CHEN Kao-shan, et al(李 江,宋国强,陈靠山,等). Chemical Journal of Chinese Universities(高等学校化学学报), 2008, 29(6): 1149. [8] Ozkan A, Yekeler M. Chemical Engineering and Processing, 2004, 43(7): 873. [9] Li W W, Zhou W Z, Zhang Y Z, et al. Bioresource Technology, 2008, 99(15): 6893. [10] XU Hui-sheng, ZHANG Tie-jun, ZHAO Guang-rong, et al(许会生,张铁军,赵广荣,等). Science and Technology of Food Industry(食品工业科技), 2007, 28(7): 197. [11] LUO Ya-jun, XIAO Xin-feng, WANG Zhao-li(罗娅君,肖新峰,王照丽). Chemistry and Industry of Forest Products(林产化学与工业), 2009, 29(1): 68. [12] Lim J M, Joo J H, Kim H O, et al. Carbohydrate Polymers, 2005, 61(3): 296. [13] Lin S, Rayson G D. Environmental Science and Technology, 1998, 32(10): 1488. [14] GU Xiao-mei, WU Hou-ming, MA Gui-rong(顾笑梅,吴厚铭,马桂荣). Chemical Journal of Chinese Universities(高等学校化学学报), 2004, 25(7): 1288. [15] Ai L, Zhang H, Guo B, et al. Carbohydrate Polymers, 2008, 74(3): 353. [16] Pradhan S, Singh S, Rai L C. Bioresource Technology, 2007, 98(3): 595.
|
[1] |
CHENG Jia-wei1, 2,LIU Xin-xing1, 2*,ZHANG Juan1, 2. Application of Infrared Spectroscopy in Exploration of Mineral Deposits: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 15-21. |
[2] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
[3] |
YANG Cheng-en1, 2, LI Meng3, LU Qiu-yu2, WANG Jin-ling4, LI Yu-ting2*, SU Ling1*. Fast Prediction of Flavone and Polysaccharide Contents in
Aronia Melanocarpa by FTIR and ELM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 62-68. |
[4] |
GAO Feng1, 2, XING Ya-ge3, 4, LUO Hua-ping1, 2, ZHANG Yuan-hua3, 4, GUO Ling3, 4*. Nondestructive Identification of Apricot Varieties Based on Visible/Near Infrared Spectroscopy and Chemometrics Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 44-51. |
[5] |
LIU Jia, ZHENG Ya-long, WANG Cheng-bo, YIN Zuo-wei*, PAN Shao-kui. Spectra Characterization of Diaspore-Sapphire From Hotan, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 176-180. |
[6] |
BAO Hao1, 2,ZHANG Yan1, 2*. Research on Spectral Feature Band Selection Model Based on Improved Harris Hawk Optimization Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 148-157. |
[7] |
GUO Ya-fei1, CAO Qiang1, YE Lei-lei1, ZHANG Cheng-yuan1, KOU Ren-bo1, WANG Jun-mei1, GUO Mei1, 2*. Double Index Sequence Analysis of FTIR and Anti-Inflammatory Spectrum Effect Relationship of Rheum Tanguticum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 188-196. |
[8] |
LI Xiao-dian1, TANG Nian1, ZHANG Man-jun1, SUN Dong-wei1, HE Shu-kai2, WANG Xian-zhong2, 3, ZENG Xiao-zhe2*, WANG Xing-hui2, LIU Xi-ya2. Infrared Spectral Characteristics and Mixing Ratio Detection Method of a New Environmentally Friendly Insulating Gas C5-PFK[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3794-3801. |
[9] |
HU Cai-ping1, HE Cheng-yu2, KONG Li-wei3, ZHU You-you3*, WU Bin4, ZHOU Hao-xiang3, SUN Jun2. Identification of Tea Based on Near-Infrared Spectra and Fuzzy Linear Discriminant QR Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3802-3805. |
[10] |
LIU Xin-peng1, SUN Xiang-hong2, QIN Yu-hua1*, ZHANG Min1, GONG Hui-li3. Research on t-SNE Similarity Measurement Method Based on Wasserstein Divergence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3806-3812. |
[11] |
SUN Wei-ji1, LIU Lang1, 2*, HOU Dong-zhuang3, QIU Hua-fu1, 2, TU Bing-bing4, XIN Jie1. Experimental Study on Physicochemical Properties and Hydration Activity of Modified Magnesium Slag[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3877-3884. |
[12] |
BAI Xue-bing1, 2, SONG Chang-ze1, ZHANG Qian-wei1, DAI Bin-xiu1, JIN Guo-jie1, 2, LIU Wen-zheng1, TAO Yong-sheng1, 2*. Rapid and Nndestructive Dagnosis Mthod for Posphate Dficiency in “Cabernet Sauvignon” Gape Laves by Vis/NIR Sectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3719-3725. |
[13] |
WANG Qi-biao1, HE Yu-kai1, LUO Yu-shi1, WANG Shu-jun1, XIE Bo2, DENG Chao2*, LIU Yong3, TUO Xian-guo3. Study on Analysis Method of Distiller's Grains Acidity Based on
Convolutional Neural Network and Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3726-3731. |
[14] |
DANG Rui, GAO Zi-ang, ZHANG Tong, WANG Jia-xing. Lighting Damage Model of Silk Cultural Relics in Museum Collections Based on Infrared Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3930-3936. |
[15] |
LUO Li, WANG Jing-yi, XU Zhao-jun, NA Bin*. Geographic Origin Discrimination of Wood Using NIR Spectroscopy
Combined With Machine Learning Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3372-3379. |
|
|
|
|