| 光谱学与光谱分析 |
|
|
|
|
|
| Study on Complex Formation of Quercetin Reacting with Cu2+ Using UV-Vis Absorption Spectroscopy |
| XUE Jun-peng1, LI Ping1*, WANG Qi-ming1, ZHAO Wei1, WU Da-cheng2 |
1. Institute of Atomic and Molecular Physics, Sichuan University, Chengdu 610065, China
2. Textile and Food College, Sichuan University, Chengdu 610065, China |
|
|
|
|
Abstract Using an intensified spectroscopic detector ICCD, the real-time UV-Vis absorption spectra of quercetin (Q) reacting with Cu2+ under neutral and acid conditions were acquired. The exposure time was 0.1 ms for each spectrum, and the molar ratio of Cu2+∶Q was 0.2, 0.5, 1.0, 2.0, 5.0 and 10.0, respectively. Results indicate that the changes in absorption bands of reaction solutions with different ratio of Cu2+∶Q were similar if other conditions were the same, and the greater the molar ratio, the shorter the reaction time; the reaction processes of complex formation were different under neutral and acid conditions. There was an intermediate product with the absorption peak at 428 nm that occurred during the reaction under neutral condition, but the final product was directly formed under acid condition, and the final products under both conditions had the same absorption bands centered at 296 nm; reaction processes with air and without air showed no difference. The present work first observed that there was an intermediate product formed during the complex formation of quercetin reacting with Cu2+ and the absorption band of the final complex was centered at 296 nm. Results obtained here provide useful experimental evidence for the study of complex mechanism of quercetin with Cu2+.
|
|
Received: 2008-05-29
Accepted: 2008-09-02
|
|
|
|
Corresponding Authors:
LI Ping
E-mail: lpscun@163.com
|
|
[1] Cornard J P, Boudet A C, Merlin J C. Spectrochimical Acta Part A, 2001, 57: 591.
[2] Deng H T, Berkel G J V. J. Mass Spectrom., 1998, 33: 1080.
[3] Murota K, Terao J. Archives of Biochemistry and Biophysics, 2003, 417: 12.
[4] Bravo A, Anacona J R. Transition Metal Chemistry, 2001, 26: 20.
[5] Torreggiani A, Trinchero A, Tamba M, et al. J. Raman Spectrosc., 2005, 36: 380.
[6] Makris D P, Rossiter J T. J. Sci. Food and Agric., 2002, 82: 1147.
[7] Aytac G, Burcu T, Makris D P. Eur. Food Res. Technol., 2007, 225: 435.
[8] Brown J E, Khodr H, Hider R C, et al. Biochem. J., 1998, 330: 1173.
[9] HUANG Liang, YU De-quan(黄 量，于德泉) . Application of UV-Spectrum in the Organic Chemistry(紫外光谱在有机化学中的应用). Beijing: Science Press(北京：科学出版社), 1998. 281.
[10] Cornard J P, Merlin J C. Journal of Molecular Structure, 2003, 651: 381.
|
| [1] |
HUANG Li, MA Rui-jun*, CHEN Yu*, CAI Xiang, YAN Zhen-feng, TANG Hao, LI Yan-fen. Experimental Study on Rapid Detection of Various Organophosphorus Pesticides in Water by UV-Vis Spectroscopy and Parallel Factor Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3452-3460. |
| [2] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [3] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
| [4] |
CI Cheng-gang*, ZANG Jie-chao, LI Ming-fei*. DFT Study on Spectra of Mn-Carbonyl Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1434-1441. |
| [5] |
HOU Qian-yi1, 2, DONG Zhuang-zhuang1, 2, YUAN Hong-xia1, 2*, LI Qing-shan1, 2*. A Study of the Mechanism of Binding Between Quercetin and CAV-1 Based on Molecular Simulation, Bio-Layer Interferometry and
Multi-Spectroscopy Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 890-896. |
| [6] |
LIU Yu-ying1, 2, WANG Xi-yuan1, 2*, MEI Ao-xue1, 2. Green Preparation of Biomass Carbon Quantum Dots for Detection of Cu2+[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 248-253. |
| [7] |
LÜ Yang1, PEI Jing-cheng1*, ZHANG Yu-yang2. Chemical Composition and Spectra Characteristics of Hydrothermal Synthetic Sapphire[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3546-3551. |
| [8] |
ZHAO Xiong-wei1, WU Dong-ming2, LI Qin-fen2, WANG Xu1*, CHEN Miao2. Response of Dissolved Organic Matter Chemical Properties to Long-Term Different Fertilization in Latosol: Insight From Ultraviolet-Visible Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3210-3216. |
| [9] |
MA Ping1, 2, Andy Hsitien Shen1*, ZHONG Yuan1, LUO Heng1. Study on UV-Vis Absorption Spectra of Jadeite From Different Origins[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1827-1831. |
| [10] |
YAN Hua1, LIU Xing-hua2, DING Yong3, ZHAO Zhi1, LUO Yong-feng1, WU Yu-hong1, YAN Peng1, DONG Lu1, WANG Da-xi4. Instantaneous Emission Spectra and Mechanism Study on the Reaction of ClF3O and n-Decane[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1522-1528. |
| [11] |
SHAO Ke-man, FU Gui-yu, CHEN Su-yan, HONG Cheng-yi, LIN Zheng-zhong*, HUANG Zhi-yong*. Preparation of Molecularly Imprinted Fluorescent Probe for Rare Earth Complex and Determination of Malachite Green Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 808-813. |
| [12] |
ZHONG Yuan, QU Meng-wen, Andy Hsitien Shen*. Comparison of Chemical Composition and Spectroscopy of Purple- Brownish Red Garnet From Zambia, Tanzania and Australia[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 184-190. |
| [13] |
YANG Yun-fan1, HU Jian-bo1, LIU Yong-gang1，2*, LIU Qiang-qiang3, ZHANG Hang4, XU Jian-jie5, GUO Teng-xiao5. DFT／TDDFT Study on Spectra of Peptides[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3172-3177. |
| [14] |
ZHANG Jiao1, 2, WANG Yuan-zhong1, YANG Wei-ze1, ZHANG Jin-yu1*. Data Fusion of ATR-FTIR and UV-Vis Spectra to Identify the Origin of Polygonatum Kingianum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1410-1416. |
| [15] |
CHEN Yu1, LIU Yu-zhu1, 2*, WANG Xing-chen3, ABULIMITI Bumaliya3*. Effect of External Electric Field on the Spectral Characteristics of Bromobenzene[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3732-3735. |
|
|
|
|
