光谱学与光谱分析 |
|
|
|
|
|
Study on Fenton-Methylene Blue Spectral Analysis Method for Determining the Inhibitory Effect of Quercetin Complexes on Hydroxyl Free Radical |
LI Fang1, ZHENG Huai-li2 |
1. College of Chemistry, Sichuan University, Chengdu 610064, China 2. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China |
|
|
Abstract In the present, Fenton-Methylene Blue spectral measurement was established, and the new analytical method was applied successfully to determine the inhibitory effect of quercetin (Que) and its complexes on hydroxyl free radical. Methylene Blue(MB) can capture the ·OH produced by Fenton reaction, turning its color from blue to achromatic. Therefore, MB was selected as the indicator of the spectral measure study. The testing conditions optimized were pH 8.0, H2O2 solution (0.3%) 0.50 mL, FeSO4 solution (5 mmol·L-1) 0.50 mL and MB solution(2.56×10-5 mol·L-1)1.0 mL. The experiment results showed that Que-Zn(Ⅱ), Que-Cu(Ⅱ) and Que-Fe(Ⅲ) complexes held significant effects of scavenging action on ·OH radical and were more effective in scavenging ·OH than quercetin itself, showing the good cooperative action between quercetin and trace metal elements. The structure-bioactivity relationship of the coordination complexes was also discussed through analyzing the antioxygenic activity of quercetin-metal complexes.
|
Received: 2005-10-16
Accepted: 2006-01-18
|
|
Corresponding Authors:
LI Fang
|
|
Cite this article: |
LI Fang,ZHENG Huai-li. Study on Fenton-Methylene Blue Spectral Analysis Method for Determining the Inhibitory Effect of Quercetin Complexes on Hydroxyl Free Radical[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2294-2297.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2006/V26/I12/2294 |
[1] HUI Rui-hua, HOU Dong-yan, GUAN Chong-xin, et al(回瑞华,侯冬岩,关崇新,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005, 25(2): 266. [2] Craig W J. American Journal of Clinical Nutrition, 1999, 70 (3):491. [3] LIN Jin-ming, QU Feng, SHAN Xiao-quan(林金明,屈 锋,单孝全). Chinese Journal of Analytical Chemistry(分析化学),2002, 30(12): 1507. [4] CHENG Guan-hua, TIAN Yi-ling, YANG Geng-liang, et al(陈冠华,田益玲,杨更亮,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002, 22(4): 634. [5] Tawa R, Sakurai H. Anal Lett., 1997, 30 (15): 2811. [6] ZENG Xiao-ling(曾小玲). China Journal of Modern Medicine(中国现代医学杂志),1999, 9(11): 22. [7] LI Gui-rong, YANG Sheng-yuan(李贵荣,杨胜圆). Chemical World(化学世界),2001, (8): 421. [8] LUO Zong-ming, FANG Yan-xiong, ZHANG Kun, et al(罗宗铭,方岩雄,张焜,等). China Oils and Fats(中国油脂),2003, 28(3): 58. [9] ZHENG Rong-liang(郑荣梁). Redical Biochemistry(自由基生物化学). Beijing: Higher Education Press(北京:高等教育出版社),1992. |
[1] |
LIU Li-zhu1, 2, DONG Fa-qin2*, SUN Shi-yong2, HE Xiao-chun2, WANG Ming-cui3 . Quantitative Detection of Hydroxyl Radical Generated in Quartz Powder/Phosphate Buffer Solution System by Fluorescence Spectrophotometry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(07): 1886-1889. |
[2] |
CAO Yan-ping1,2, YUAN Ying-mao1,2, ZHU Yu-chen1,2 . Study on the Spectrophotometric Determination of Hydroxyl Free Radical from Low Power Trench-Type Ultrasound [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(05): 1320-1323. |
[3] |
GUO Xue-feng1, YUE Yong-de1*, MENG Zhi-fen2, TANG Feng1, WANG Jin1, YAO Xi1, XUN Hang1, SUN Jia1 . Detection of Antioxidative Capacity of Bamboo Leaf Extract by Scavenging Hydroxyl Free Radical[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 508-511. |
[4] |
YANG Xian-li, BAI Min-dong, HAN Feng, GU Jian-long. Study on the Degradation of Phenol Wastewater Using Hydroxyl Radical Produced by Plasma and the Course by UV Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(11): 2649-2652. |
[5] |
ZHANG Hai-rong,WANG Wen-yan. Study of Scavenging Activity of Sorghum Pigment to Hydroxyl Free Radicals by Fluorimetry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 547-551. |
[6] |
LIANG Ai-hui1, JIANG Zhi-liang1*, ZHOU Su-mei2, ZHOU Yu-chan2, LIANG Yue-yuan2, CHEN Li-yu2 . Rhodamine 6G Association Particle Based Spectrophotometric Determination of Hydroxy Free Radical and Its Application to the Selection of Antioxidant[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(11): 2113-2115. |
[7] |
LU Feng, XIAO Jie, LIN Pei-ying, YANG Gen-jin. Convolution Spectrometric Determination of the Effect of Hydroxyl Radical Scavenger on UV Radiation-induced DNA Aberrance [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 82-84. |
|
|
|
|