| 光谱学与光谱分析 |
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| Study of Interaction of Ciprofloxacin Probe and Calf Thymus DNA Using Fluorescence Spectrometry |
| GAN Ning1, 2, GE Cong-xin1 |
1. College of Material Science and Chemical Engineering, Department of Chemistry, Ningbo University, Ningbo 315211, China
2. State Key Laboratory of Coordination Chemistry, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China |
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Abstract The interaction of ciprofloxacin and calf thymus DNA (ctDNA) was studied using UV spectrometry and fluorescence spectrometry. Ciprofloxacin exhibited a fluorescence excitation at 270 nm and a fluorescence emission at 420 nm in pH 7.0 phosphate buffer solutions due to the groove binding of ciprofloxacin on calf thymus ctDNA. The binding constant was 2.64×104 mol-1·L(25 ℃). Based on the fluorescence quenching, a novel method for sensitive determination of calf thymus ctDNA concentration ranging from 80 nmol·L-1 to 45 μmol·L-1 was developed. The relative standard deviation for eleven detections of 25 μmol·L-1 ctDNA was 4.2%.
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Received: 2005-10-16
Accepted: 2006-02-28
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Corresponding Authors:
GAN Ning
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| Cite this article: |
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GAN Ning,GE Cong-xin. Study of Interaction of Ciprofloxacin Probe and Calf Thymus DNA Using Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2276-2279.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I12/2276 |
[1] WANG Li-sheng, ZOU Jie-ming, JIANG Zhi-liang, et al(王力生, 邹节明, 蒋治良, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(12)：1640.
[2] ZHANG Hong-yi，LIU Bao-sheng, ZHANG Hong-lei, et al(张红医，刘保生，张红蕾, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(6)：1054.
[3] Wu S Q, Zhang W J, Chen X G, et al. Spectrochim. Acta Part A, 2001, 57: 1317.
[4] Ghosh J K, Mandal A K, Pal M K. Spectrochim. Acta Part A, 1999, 55: 1877.
[5] Cao Y, He X W. Spectrochim. Acta Part A, 1998, 54: 883.
[6] Jin W J, Liu W H, Yang X. Microchem J., 1999, 61: 115.
[7] Xu C, He P G, Fang Y Z. Anal. Chim. Acta, 2000, 411: 31.
[8] JIANG Yun-bao, HUANG Xian-zhi, CHEN Guo-zhen(江云宝，黄贤智，陈果珍). Acta Physico-Chemica Sinica(物理化学学报)，1991，3(7)：289.
[9] Karlovsky P, Decock A W. Anal. Biochem., 1991, 194: 192.
[10] Harshman K D, Dervan P B. Nucleic Acids Res., 1985, 13: 4825.
[11] Cao Y, He X W, Gao Z, et al, Talanta, 1999, 49: 377.
[12] Marin D, Perez P, Teijeiro C, et al. Biophysical Chem., 1998, 75(4): 87.
[13] JIANG Zhi-liang, LIU Shao-pu, ZHAO Bao-gang, et al(蒋治良，刘绍璞，赵保刚, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(4)：615. |
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