| 光谱学与光谱分析 |
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| Study of Fluorescence Spectra of Blattela Germanica Allergen, Derenatured and Renatured Bla g 2 |
| ZHU Jian-qi1, LIU Zhi-gang1*, HUANG Hai-zhen2, ZHU Yong-feng1, XU Hong1 |
1. College of Life Science, Shenzhen University, Shenzhen 518060, China
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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Abstract Recombinant proteins extracted from inclusion body remain in denaturation status. Renaturation in vitro after initial purification is a key step of downstream processing. A common method of renaturation of recombinant proteins is the dilution method. With Bla g 2 as a model protein, the conformational changes of denatured and renatured Bla g 2 were investigated by applying fluorescence spectra. The effects of different urea concentrations, different SDS concentrations and different pH on the fluorescence intensity of renatured protein were also investigated. The reasons for these were studied with the knowledge of molecular structure.
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Received: 2005-11-06
Accepted: 2006-02-20
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Corresponding Authors:
LIU Zhi-gang
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| Cite this article: |
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ZHU Jian-qi,LIU Zhi-gang,HUANG Hai-zhen, et al. Study of Fluorescence Spectra of Blattela Germanica Allergen, Derenatured and Renatured Bla g 2[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2254-2257.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I12/2254 |
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