光谱学与光谱分析 |
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Investigation of Fucoidanase by FTIR Spectra |
MA Shuang1,2,XIAO Hou-rong2,WU Qian-qian2,PAN Ren-rui2,CAI Jing-min1,2* |
1. College of Life Science,Anhui Agricultural University,Hefei 230036,China 2. Department of Biology and Environment Engineering,Hefei College,Hefei 230022,China |
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Abstract Fucoidanase was isolated and purified from marine fungus LD8 by solid state fermentation,extraction with citric acid buffer,acetone precipitatation and column chromatography on Sephadex G-100. A single band on PAGE shows that pretty pure fucoidanase has been obtained. FT-IR spectra and its derivation,self-deconvolution and curve-fitting methods were used to analyze the secondary structure of the fucoidanase. Composite bands of the amide Ⅰ and amide Ⅲ were studied by using Fourier self-deconvolution (FSD) with an enhancement factor of K=2.2 and a half width of 20.2 cm-1. The relative average fractions of α-helix,β-sheet,random coil,β-turn are 11.5%,58.6%,14.5% and 15.9%,respectively,according to amide Ⅰ region,while the content of α-helix is 12%,β-sheet 57.3%,random coil 14.5%,and β-turn 16.3% on amide Ⅲ region. In other words,both the conclusions were exactly consistent. All the above results show that β-sheet was the dominant component,which is about 58%,and that β-turn is about 15%,random coil 15%,and α-helix 12% at room temperature.
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Received: 2006-11-10
Accepted: 2007-02-20
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Corresponding Authors:
CAI Jing-min
E-mail: caijingmin@hfuu.edu.cn
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[1] Chevolot L,Foucault A,Chaubet F,et al. Carbohydrate Res.,1999,319:154. [2] Mauray S,de Raucourt E,Talbot J,et al. Biochim. Biophys. Acta,1998,1378:184. [3] Nishino T,Yamauchi T,Horie M,et al. Thromb. Res.,2000,99:623. [4] Baba M,Snoeck R,Pauwels R,et al. Antimicrob. Agents Chemother.,1988,32: 1742. [5] Lynch G,Low L,Li S,et al. J. Leukocyte Biology,1994,56(3) :266. [6] YANG Chi-ming(杨池明). Chem. J. Chinese Universities(高等学校化学报),2002,23(2):243. [7] JIANG Jun-guang,WANG Zhen-xin,LIU Chang-wei,et al(蒋俊光,王振新,刘长伟,等). Chem. J. Chinese Universities(高等学校化学报),2001,22(7):1131. [8] WANG Bin,WANG Jing,YU Jiang,et al(王 斌,王 靖,余 江,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),1999,19(5):674. [9] Parvez I H,Feride S J. Molecular Catalysis B,Enzymatic,1999,7:207. [10] Xie Mengxia. Spectroscopy Letters,1996,29(1):53. [11] Xie Mengxia,Liu Yuan. Spectrochimica Acta PartA,2002,58(13):2817. [12] Wu Ke,Cai Jingmin,et al. Marine Biotechnology an Overview of Leading Fields,in Ⅸth ESMB Meeting,Nattes,12-14,May,2003,122. [13] Xiao Hourong,Xie Yongshu,Liu Qingliang,et al. Spectrochimica Acta Part A,2005,61:2840. [14] Byler D M,Brouillette J N,Susi H. Spectroscopy,1986,1(3): 29. [15] YANG Jiao-lan,LUO Tian(杨姣兰,罗 添). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002,22(4):610. [16] Dong Aichun,Huang Ping,Caughey W S. Biochemistry,1990,29:3303. [17] Singh B R,DeOliveira D B,Fu F N,et al. Proc. Biomol. Spectr. ⅢSPIE,1993,1890:47. |
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