| 光谱学与光谱分析 |
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| Study on Temperature & EMF Co-Effects on Insulin Conformation and Biological Functions by Fluorescence and Raman Spectroscopy |
| YAN Zhe,CHEN Shu-de*,QIAO Deng-jiang |
| Key Laboratory of Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China |
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Abstract Our previous studies had suggested that the intercellular signal molecule might be an important target of electromagnetic fields. Insulin, an intercellule signal molecule, plays a critical role in transferring life information. The studies on effects of pulsed electric fields (PEF) on insulin molecule are meaningful for explaining the mechanism of biological effects of electromagnetic fields. The PEF, which we used, with its highest electric field (2×106 V·m-1) coupled into the insulin buffer, was about 1 V·cm-1cm, with a repeating frequency of 50 Hz. In the present study, the changes of insulin conformation induced by PEF were studied by fluorescence spectroscopy. Insulin solution was exposed to 50 Hz PEF with different electric field intensities for 5-35 min, which caused a time-and dose-dependent decrease in fluorescence intensities of insulin. Further, insulin solution was exposed to PEF at different temperatures to investigate the effects of PEF co-operated with temperature on insulin. The results indicated that the difference in temperature (about 5 ℃) could induce conflict results，which is due to the effects of PEF co-operated with temperature rather than only to the effect of temperature. The authors calculated that the increase in temperature induced by PEF was 0.07 ℃ (less than 0.1 ℃). So the effects of PEF were scarcely explained by thermal effects, it belongs to “non-thermal effects” of electric fields. So it was concluded that temperature is a considerably important factor in “non-thermal effects” of electric fields, and the ignorance of variety of temperature probably result in the contrary conclusion. Further, Raman spectroscopy was used to investigate the details of structure of insulin treated by PEF co-operated with temperature. The results of Raman spectroscopy verified the effects of PEF co-operated with temperature on insulin. And the reductions of the S—S band intensity at 510 cm-1, the skeletal C—C stretch band intensity at 934 cm-1, and the content of the secondary structure of the alpha helix were observed. Both S—S linkages and alpha helix structure were important to the stabilization of insulin conformation. Modification of insulin may change the biological activity either by reducing the affinity of the hormone for the receptor or by decreasing the ability of the complex, when formed, to elicit a biological response.
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Received: 2007-05-10
Accepted: 2007-08-20
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Corresponding Authors:
CHEN Shu-de
E-mail: sdchen@phy.ecnu.edu.cn
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