| 光谱学与光谱分析 |
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| Fluorescence Study on the Interactions between G3.0 PAMAM Dendrimers and Bovine Serum Albumin |
| HE Hua1, 4, LI Shan-shan1, LU Jin-rong2, GU Yan1, Chuong Pham-Huy3 |
1. Division of Analytical Chemistry，China Pharmaceutical University，Nanjing 210009, China
2. Division of Organic Chemistry，China Pharmaceutical University，Nanjing 210009, China
3. Faculty of Pharmacy, University of Paris V, 4 Avenue de l’Observatoire, 75006 Paris, France
4. Key Laboratory of Drug Quality Control and Pharmacovigilance，Ministry of Education，China Pharmaceutical University，Nanjing 210009, China |
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Abstract The interaction between amine terminated G3.0 PAMAM dendrimers and bovine serum albumin (BSA) under physiological condition was studied by fluorescence spectroscopy. Our experiments demonstrated that the fluorescence intensity of BSA decreased after the addition of G3.0 PAMAM dendrimers and the quenching mechanism was suggested as static quenching according to the Stern-Volmer equation. The binding constant of G3.0 PAMAM dendrimers with BSA was calculated to be 1.067±0.025 L·mmol-1. At the same time, synchronous fluorescence and red edge excitation shift(REES)were adopted to review the conformational changes of BSA influenced by G3.0 PAMAM dendrimers, which provides important significance for clinical medication. And the results indicated that G3.0 PAMAM dendrimers can change the conformation of BSA. Furthermore, this article also examined the influence of pH and ionic strength on the interactions, from which we can conclude that electrostatic interaction played major roles in the binding process. In conclusion, the fluorescence method is a highly sensitive and convenient way to study intermolecular interaction. Further investigation in this field will provide more important information for understanding the pharmacological effects and toxicities of drugs in human body.
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Received: 2008-09-26
Accepted: 2008-12-26
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Corresponding Authors:
HE Hua
E-mail: dochehua@163.com
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[1] Tomalia D A, Baker H, Dewald J R, et al. Polymer Journal, 1985, 17(1): 117.
[2] Jonathan D Eichman, Anna U Bielinska, Jolanta F Kukowska-Latallo, et al. Pharmaceutical Science & Technology Today, 2000, 3(7): 232.
[3] ChengYiyun, Xu Tongwen. Medicinal Chemistry, 2005, 40(11): 1188.
[4] Man Na, Cheng Yiyun, Xu Tongwen, et al. Medicinal Chemistry, 2006, 41(5): 670.
[5] Cheng Zhengjun, Zhang Yuntao. Molecular Structure, 2008, 879(1-3): 81.
[6] Hu Yanjun, Liu Yi, Sun Tingquan, et al. Biological Macromolecules, 2006, 39(4-5): 280.
[7] ZHANG Zhi-heng, LI Cai-rui, MA Jing-jun, et al(张志恒, 李彩瑞, 马晶军, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(5): 1135.
[8] Ware W R. Process J. Phys. Chem., 1962, 66(3): 455.
[9] Sun Shaofa, Zhou Bo, Hou Hanna. Biological Macromolecules, 2006, 39(4-5): 197.
[10] Callis P R. Methods Enzymology, 1997, 278: 113.
[11] Yang Yumin, Hua Qiuluan, Fan Yanli, et al. Spectrochimica Acta Part A, 2008, 69(2): 432.
[12] Demchenko A P. Trends in Biochemical Sci., 1988, 13(10): 374.
[13] Patra S K, Pal M K. Spectrochim Acta A Mol Biomol Spectrosc., 1997, 53A (10): 1609.
[14] Wang Feng, Huang Wei, Dai Zhongxiang. Molecular Structure, 2008, 875(1-3): 509. |
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