光谱学与光谱分析 |
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Application of Two-Photon Excitation Fluorescence Imaging to Real-Time Investigation of Mouse Preimplantation Embryo |
CHENG Bing, LIN Dan-ying, WANG Xiao-guang, CHEN Die-yan, MA Wan-yun* |
The Key Laboratory of Atomic and Molecular Nanosciences, Ministry of Educatioin, Tsinghua University, Beijing 100084, China |
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Abstract The blue shift of a two-photon excitation absorption peak allows the application of a single wavelength to the simultaneous excitation of several fluorochromes with disparate emission characteristics. An output at 730nm of a mode-locked femtosecond Ti-sapphire laser was used to excite four different commonly used fluorochromes, namely Hoechst 33342, Fluo-4, PI and Indo-1, and characteristic fluorescence images were obtained using (455±15)nm, (540±15)nm, (580±16)nm and (500±15)nm filters respectively. An approach to exciting with a single wavelength, staining with two different fluorochromes, and collecting fluorescence in two separate channels was employed to study mouse preimplantation embryos by 3D and 4D real-time imaging. Combined with the merits of two-photon excitation fluorescence imaging, such as better penetration, less photon-damage, and higher signal-to-noise ratio, this approach was supposed to be a novel multi-parameter investigating tool for mouse preimplantation embryos development study.
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Received: 2005-01-08
Accepted: 2005-04-18
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Corresponding Authors:
MA Wan-yun
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Cite this article: |
CHENG Bing,LIN Dan-ying,WANG Xiao-guang, et al. Application of Two-Photon Excitation Fluorescence Imaging to Real-Time Investigation of Mouse Preimplantation Embryo[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 193-197.
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URL: |
https://www.gpxygpfx.com/EN/Y2006/V26/I02/193 |
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