叶绿体延迟荧光中730 nm成分产生机理的光谱学研究

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Abstract Charge recombination in reaction center of photosystem Ⅱ(PSⅡ) is regarded as the origination of delayed fluorescence (DF). The mechanism for 730 nm component appearing in the DF spectrum of chloroplast was studied with different spectral analysis methods. Experimental results of the delayed fluorescence spectrum at different chloroplast concentration showed that the apexes at 685 and 730 nm ascend when the concentration is relative low; the peak value at 685 nm reaches a maximum when the concentration is 7.8 μg·mL^-1, however, the apex at 730 nm is still increasing. The peak value at 730 nm finally reaches a maximum at chloroplast concentration of 31.2 μg·mL^-1, while the apex at 685 nm has apparently decreased. The results of absorption spectrum showed that the ratios of A₆₈₅ to A₇₃₀ are almost a constant during the process of increasing chloroplast concentration. Furthermore, the excitation spectrum for 730 nm fluorescence shows that the 685 nm light has a high excitation efficiency. Above experimental results indicated that the 730 nm component of DF spectrum is the fluorescence of chlorophyll a in PSⅠ reaction center excited by 685 nm DF. Meanwhile, this conclusion was further verified by the invariability of DF spectrum at different delay time (1-9 s).

Key words：Chloroplast;Delayed fluorescence;PSⅡ;PSⅠ;Reabsorption

Received: 2004-08-20 Accepted: 2005-01-20

ZTFLH:

Q947

Corresponding Authors: XING Da

Cite this article:

WANG Cheng-long,XING Da,ZENG Li-zhang. Spectroscopic Research on the Mechanism for 730 nm Component in Delayed Fluorescence of Chloroplast[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 6-10.

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https://www.gpxygpfx.com/EN/Y2006/V26/I01/6