| 光谱学与光谱分析 |
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| Fluorescence Polarization Used to Derive Cell Membrane Fluidity during Photodynamic Therapy |
| WEI Rong-hui, HUANG Yan-ping, LI Shan-shan,QI Chun-yuan |
| Key Laboratory for Optical and Magnetic Resonance Spectroscopy Department of Physics, East China Normal University, Shanghai 200062, China |
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Abstract With metal phthalocyanine used as the photosensitizer, the cell membrane fluidity under the conditions of different irradiation time in photodynamic therapy was investigated by fluorescence polarization. Results show that with the photosensitizer phthalocyanine excited，the fluorescence polarization of the fluorophose was increased and the cancer cell membrane fluidity was decreased due to the photodynamic therapy. Cell proliferation was also studied by MTT. Results show that cell membrane fluidity and cell proliferation are influenced to the same extent, suggesting that cell membrane is one of the binding sites in PDT.
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Received: 2004-07-16
Accepted: 2004-11-29
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Corresponding Authors:
WEI Rong-hui
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| Cite this article: |
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WEI Rong-hui,HUANG Yan-ping,LI Shan-shan, et al. Fluorescence Polarization Used to Derive Cell Membrane Fluidity during Photodynamic Therapy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(11): 1827-1829.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I11/1827 |
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