| 光谱学与光谱分析 |
|
|
|
|
|
| Interaction between Carbaryl and Calf Thymus DNA Enhanced via Cu2+ |
| ZHANG Xiao-ming1,LI Guo-xue2,LI Dong-hao1,MIN Shun-geng1,SUN Ying1* |
1. College of Science, China Agricultural University，Beijing 100094, China
2. College of Resource and Environment, China Agricultural University，Beijing 100094, China |
|
|
|
|
Abstract The interaction between carbaryl and calf thymus DNA with Cu2+ was studied using fluorescence spectroscopy(FS) and UV spectrum. The quenching process was proved to be single static quenching and the quenching constant decreases with temperature increasing. The experimental results showed that the carbaryl can be intercalated into the twin-screw structure of calf thymus DNA, forming ctDNA-carbaryl adducts. The UV spectrum showed that ctDNA can also result in the increasing color and red shift. In the presence of Cu2+,it was discovered that the apparent association constant (Kα) increases and the binding sites number (n) of carbaryl molecules on ctDNA obviously increases. The interaction of the carbaryl and ctDNA was driven mainly by electrostatic force which was enhanced by Cu2+,thus the contribution of ΔH to ΔG increased in the presence of Cu2+. So the interaction between carbaryl and calf thymus DNA was enhanced via Cu2+.
|
|
Received: 2005-12-16
Accepted: 2006-03-28
|
|
|
|
Corresponding Authors:
SUN Ying
E-mail: Sunybj@sohu.com
|
|
| Cite this article: |
|
ZHANG Xiao-ming,LI Guo-xue,LI Dong-hao, et al. Interaction between Carbaryl and Calf Thymus DNA Enhanced via Cu2+[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 517-520.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2007/V27/I03/517 |
[1] Worthing C R, Hance R J. In the Pesticide Manual: A World Compendium. 7th ed, BCPC Berks，UK：Publications，1983.
[2] Lechner D W，Abdelz-Rahman M S. Arch. Environ. Contam. Toxicol.，1985，14：451.
[3] Bavari S，et al. Fundam. Appl. Toxicol.，1991，17：61.
[4] ZHANG Li-jin, MIN Shun-geng, SUN Ying，et al(张立金, 闵顺耕, 孙 英，等). Environmental Chemistry(环境化学), 2004, 23(5): 520.
[5] YANG Pin, GAO Fei(杨 频, 高 飞). The Principles of Bioinorganic Chemistry(生物无机化学原理). Beijing: Science Press(北京: 科学出版社), 2002.
[6] LIU Xue-feng, XIA Yong-mei, FANG Yun, et al(刘雪锋, 夏咏梅, 方 云，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8): 1301.
[7] ZHANG Li-jin, MIN Shun-geng, LI Guo-xue, et al(张立金，闵顺耕，李国学，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(5): 739.
[8] LU Ji-xin, ZHANG Yong, ZHANG Gui-zhu, et al(卢继新, 张 勇, 张贵珠，等). Journal of Analytical Science(分析科学学报), 2000, 16(6): 445.
[9] Philip D Rossand, Sabramarian S. Biochemistry, 1981, 20: 3096.
[10] Xia J L, Paul L D, Yesook K. J. Phys. Chem., 1992, 96: 6805. |
| [1] |
LIANG Ye-heng1, DENG Ru-ru1, 2*, LIANG Yu-jie1, LIU Yong-ming3, WU Yi4, YUAN Yu-heng5, AI Xian-jun6. Spectral Characteristics of Sediment Reflectance Under the Background of Heavy Metal Polluted Water and Analysis of Its Contribution to
Water-Leaving Reflectance[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 111-117. |
| [2] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [3] |
LIU Hong-wei1, FU Liang2*, CHEN Lin3. Analysis of Heavy Metal Elements in Palm Oil Using MP-AES Based on Extraction Induced by Emulsion Breaking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3111-3116. |
| [4] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [5] |
CHENG Fang-beibei1, 2, GAN Ting-ting1, 3*, ZHAO Nan-jing1, 4*, YIN Gao-fang1, WANG Ying1, 3, FAN Meng-xi4. Rapid Detection of Heavy Metal Lead in Water Based on Enrichment by Chlorella Pyrenoidosa Combined With X-Ray Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2500-2506. |
| [6] |
ZHANG Xia1, WANG Wei-hao1, 2*, SUN Wei-chao1, DING Song-tao1, 2, WANG Yi-bo1, 2. Soil Zn Content Inversion by Hyperspectral Remote Sensing Data and Considering Soil Types[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2019-2026. |
| [7] |
TANG Quan1, ZHONG Min-jia2, YIN Peng-kun2, ZHANG Zhi3, CHEN Zhen-ming1, WU Gui-rong3*, LIN Qing-yu4*. Imaging of Elements in Plant Under Heavy Metal Stress Based on Laser-Induced Breakdown Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1485-1488. |
| [8] |
ZHANG Chao1*, SU Xiao-yu1, XIA Tian2, YANG Ke-ming3, FENG Fei-sheng4. Monitoring the Degree of Pollution in Different Varieties of Maize Under Copper and Lead Stress[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1268-1274. |
| [9] |
CHEN Ping-yun1, KANG Xiu-tang1, GUO Liang-qia2*. Study of Emission Characteristics of Particulate Arsenic, Cadmium, Copper and Lead Derived From Burning of Tibetan Incenses by
ICP-OES Method With Microwave Digestion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 419-425. |
| [10] |
ZHU Zhao-zhou1, YAN Wen-rui1, 2, ZHANG Zi-jing1, 2. Research of Pollution Characteristics, Ecological and Health Risks of Heavy Metals in PM2.5 From Fireworks by Inductively Coupled
Plasma-Mass Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 644-650. |
| [11] |
TANG Ju1, 2, DAI Zi-yun2*, LI Xin-yu2, SUN Zheng-hai1*. Investigation and Research on the Characteristics of Heavy Metal Pollution in Children’s Sandpits Based on XRF Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3879-3882. |
| [12] |
LIU Hong-jun1, NIU Teng1, YU Qiang1*, SU Kai2, YANG Lin-zhe1, LIU Wei1, WANG Hui-yuan1. Inversion and Estimation of Heavy Metal Element Content in Peach Forest Soil in Pinggu District of Beijing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3552-3558. |
| [13] |
JUMAHONG Yilizhati1, 2, TAN Xi-juan1, 2*, LIANG Ting1, 2, ZHOU Yi1, 2. Determination of Heavy Metals and Rare Earth Elements in Bottom Ash of Waste Incineration by ICP-MS With High-Pressure Closed
Digestion Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3168-3173. |
| [14] |
WU Bing, YANG Ke-ming*, GAO Wei, LI Yan-ru, HAN Qian-qian, ZHANG Jian-hong. EC-PB Rules for Spectral Discrimination of Copper and Lead Pollution Elements in Corn Leaves[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3256-3262. |
| [15] |
LI Jun-meng1, ZHAI Xue-dong1, YANG Zi-han1, ZHAO Yan-ru1, 2, 3, YU Ke-qiang1, 2, 3*. Microscopic Raman Spectroscopy for Diagnosing Roots in Apple
Rootstock Under Heavy Metal Copper Stress[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2890-2895. |
|
|
|
|
