| 光谱学与光谱分析 |
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| Interaction of ctDNA and EGCG-Cu(Ⅱ) Complex |
| WANG Dong-dong1, 2,MENG Qing-xiang1,CHEN Dan-dan1,REN Li-ping1*,ZHOU Zhen-ming1,MU Hao-jie1 |
1. College of Animal Science/Beef Cattle Research Center, China Agricultural University, State Key Laboratory of Animal Nutrition, Beijing 100094, China
2. College of Science, North China University of Technology, Beijing 100041, China |
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Abstract The fluorescence spectra, absorption spectra and resonance light scattering (RLS) spectra of EGCG-Cu(Ⅱ) complex with nucleic acid were studied. Compared with the spectrum of EGCG-Cu(Ⅱ), the spectrum of EGCG-Cu(Ⅱ)-DNA system showed the following features: (1) in the fluorescence spectra, the peak did not shift, but the fluorescence intensity was enhanced and was proportional to the concentration of DNA. Under suitable conditions, EGCG-Cu(Ⅱ) promised to be a probe for detecting DNA; (2) Absorption spectra of EGCG-Cu(Ⅱ)-DNA system showed hyperchromicity; and (3) RLS of EGCG-Cu(Ⅱ)-DNA system was enhanced also. A mechanism was suggested that the combination of nucleic acid and EGCG-Cu(Ⅱ) complex was caused by intercalation and electrostatic force. An intercalation interaction between EGCG-Cu(Ⅱ) and DNA enhanced the fluorescence intensity. Hyperchromicity of absorption spectra and RLS occurred because of electrostatic force. The effects of acid and ionic strength on fluorescence intensity were also discussed.
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Received: 2005-06-10
Accepted: 2006-04-20
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Corresponding Authors:
REN Li-ping
E-mail: renlp@cau.edu.cn
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| Cite this article: |
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WANG Dong-dong,MENG Qing-xiang,CHEN Dan-dan, et al. Interaction of ctDNA and EGCG-Cu(Ⅱ) Complex[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 510-513.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I03/510 |
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