光谱学与光谱分析 |
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Study on the Interaction between Brucine and Bovine Serum Albumin |
WANG Chun, WU Qiu-hua, WANG Zhi*,HAN Dan-dan, SONG Shuang-ju |
College of Science, Agricultural University of Hebei, Baoding 071001, China |
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Abstract The interaction between brucine and bovine serum albumin (BSA) was investigated using fluorescence spectroscopy (FS) and ultraviolet spectroscopy (UV). The experimental results showed that the brucine quenches the inner fluorescence by forming a brucine-BSA complex. It was found that both static quenching and non-radiation energy transfer were the main reasons for the fluorescence quenching. The apparent binding constants (KA) between brucine and BSA were 6.3×103 (27 ℃) and 7.7×103 (37 ℃),and the binding sites (n) were 0.94(27 ℃) and 0.97 (37 ℃). According to the Frster theory of non-radiation energy transfer, the binding distances (r) were also obtained. The process of binding was a spontaneous molecular interaction in which entropy increased and Gibbs free energy decreased, indicating that the interaction between brucine and BSA was driven mainly by hydrophobic force.
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Received: 2005-12-18
Accepted: 2006-03-28
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Corresponding Authors:
WANG Zhi
E-mail: wangzhi@mail.hebau.edu.cn
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Cite this article: |
WANG Chun,WU Qiu-hua,WANG Zhi, et al. Study on the Interaction between Brucine and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(04): 754-757.
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URL: |
https://www.gpxygpfx.com/EN/Y2007/V27/I04/754 |
[1] GU Zhen-lun, ZHAO Xue-ying(顾振纶,赵雪英). Acta Pharmacologica Sinica(中国药理学报),1996,17(5): 442. [2] Rost B. Structure, 1998,6: 259. [3] LIU Xue-feng, XIA Yong-mei, FANG Yun, et al(刘雪锋,夏咏梅,方 云,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(8): 1301. [4] WU Gen-hua, WANG Chun-hua(吴根华,汪春华). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(2): 246. [5] LI Xiao-tian, ZHU Yan-na, WENG Shi-ai(李晓天, 朱燕娜, 翁世艾). J. Henan Med. Univer.(河南医科大学学报),1998,33(1): 40. [6] YIN Jian(阴 健). Modern Research and Clinical Application of Traditional Chinese Medicine(中药现代研究与临床应用). Beijing: Xueyuan Press(北京:学苑出版社),1993. 118. [7] CHA Dan-ming,LI Shu-ting,YANG Yong-fei,et al(查丹明, 李舒婷, 杨勇飞, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),1999,19(6): 798. [8] XIAO Hou-rong,SHENG Liang-quan,SHI Chun-hua,et al(肖厚荣, 盛良全, 施春华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(1): 78. [9] YANG Pin,GAO Fei(杨 频, 高 飞). The Principles of Bioinorganic Chemistry (生物无机化学原理). Beijing: Science Press(北京:科学出版社),2002. 121. [10] YANG Man-man,YANG Pin,ZHANG Li-wei(杨曼曼, 杨 频, 张立伟). Chinese Science Bulletin(科学通报),1997,42(12): 1276. [11] FENG Xi-zeng,JIN Rui-xiang,QU Yun,et al(冯喜增, 金瑞祥, 曲 芸, 等). Chem. J. Chinese Univer.(高等学校化学学报),1996,17(6): 866. [12] Ross P D,Subramanian S. Biochemistry,1981,20: 3096.
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