光谱学与光谱分析 |
|
|
|
|
|
Determination of the Content of Poly(Lactic Acid-co-Phenylalanine) by Infrared Spectroscopy |
LIU Ying, WEI Rong-qing, LIU Xiao-ning*,WANG Ming |
College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China |
|
|
Abstract To improve the thermal stability of poly (lactic acid), poly (lactic acid-co-phenylalanine) was prepared by introducing the phenyl of phenylalanine to the side chain. 3-phenylmethyl-2,5-morpholinedione (PMD) and lactide (LA) were synthesized using phenylalanine and lactic acid as raw materials, respectively. Subsequently, poly (lactic acid-co-phenylalanine), homopolymer of PMD (PPMD) and homopolymer of LA (PLA) were obtained by ring-opening polymerization of PMD and LA as monomer. The amount of phenyl had an effect on the thermal stability, so a quantitative analysis by infrared spectroscopy was established in the present paper. Two homopolymers were determined by infrared spectroscopy to find out two characteristic peaks at 1 671.53 and 870.82 cm-1,which were selected for PPMD and PLA, respectively. Choosing the characteristic peaks and according to the Lambert-Beer law, which is the linear relationship between absorbance and concentration of an absorbing species, a standard working curve was calculated: y=0.055 67x+0.109 1 (r=0.999 3), where x was the reciprocal of PPMD monomer molar concentration and y was the absorbance ratio of PLA to PPMD at their characteristic peaks. Collecting the absorbance of poly (lactic acid-co-phenylalanine) at two characteristic peaks, the contents of copolymer were calculation by the working curve and the results were similar to that of 1H NMR by calculation from the ratio of proton area of PMD and PLA. It was noted that the relative error was below 2%. It was also confirmed that the content of copolymer could be determined by infrared spectroscopy conveniently and quickly. Meanwhile, this method could be adopted to determine the other poly (lactic acid-co-amino acid) practicably and widely.
|
Received: 2007-11-02
Accepted: 2008-02-12
|
|
Corresponding Authors:
LIU Xiao-ning
E-mail: xiaoningliu@163.com
|
|
[1] Helder J, Kohn F E, Sáto S, et al. Makromolckulare Chemie, Rapid Communication, 1985, 6(1): 9. [2] Ouchi T, Seik H, Nazaki T, et al. Polymer Science: Part A. Polymer Chemistry, 1998, 36: 1283. [3] Ohya Y, Matsunami H, Yamabe E, et al. Journal of Biomedical Materials Research, 2003, 65A(1): 79. [4] Ouchi T, Miyazaki H, Arimura H. Journal of Polymer Science: Part A: Polymer Chemistry, 2002, 40(9): 1218. [5] Ouchi T, Ohya Y. Journal of Polymer Science Part A: Polymer Chemistry, 2004, 42(3): 453. [6] Lavik E B, Hrkach J S, Lotan N, et al. Journal of Biomedical Materials Research, 2001, 58(3): 291. [7] Ouchi T, Miyazaki H, Arimura H, et al. J. Polymer Science: Part A. Polymer Chemistry, 2002, 40(10): 1426. [8] Deng X M, Yuan M L, Yuan M L. European Polymer Journal, 2002, 38(7): 1435. [9] Liu Y, Yuan M, Deng X. European Polymer Journal, 2003, 39(5): 977. [10] Liu X N, Bayer E, Xue G.Spectroscopy Letters, 1997, 30(2): 289. [11] Liu X N, Deyle C, Wei R Q, et al. Applied Spectroscopy, 1999, 53(1): 119. [12] Liu X N, Chen J L, Wei R Q, et al. Spectroscopy Letters, 2000, 33(6): 867. [13] WANG Wen-bo, SHEN Shu-chang, ZHANG Li-yuan, et al(王文波,申书昌,张丽媛,等). Spectroscopy and Spectal Analysis(光谱学与光谱分析), 2007, 27(6): 1121. [14] CHEN Yun-kui(陈允魁). Infrared Spectroscopy and Application(红外吸收光谱法及其应用). Shanghai:Shanghai Jiaotong University Press(上海: 上海交通大学出版社),1993. 69, 85. [15] Fu N F, Raymond C G. U S. Patent, 1987, 4916209. [16] Kulkarni R K, Pani K C, Neuman C, et al. Archives of Surgery, 1966, 93: 839. [17] Kister G, Cassanas G, Vert M. Polymer, 1998, 39(2): 267. [18] Wang D, Feng X D. Macromolecules, 1997, 30(19): 5688. [19] Barrera D A, Zylstra E, Lansbury P T, et al. Macromolecules, 1995, 28(2): 425.
|
[1] |
XU Rong1, AO Dong-mei2*, LI Man-tian1, 2, LIU Sai1, GUO Kun1, HU Ying2, YANG Chun-mei2, XU Chang-qing1. Study on Traditional Chinese Medicine of Lonicera L. Based on Infrared Spectroscopy and Cluster Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3518-3523. |
[2] |
LI Chen-xi1, SUN Ze-yu1, 2, ZHAO Yu2*, YIN Li-hui2, CHEN Wen-liang1, 3, LIU Rong1, 3, XU Ke-xin1, 3. The Research Progress of Two-Dimensional Correlation Spectroscopy and Its Application in Protein Substances Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 1993-2001. |
[3] |
SUN Yan-hua1, 2, FAN Yong-tao1, 2*. Correction of Temperature Influence in Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1690-1695. |
[4] |
WEI Gang1, GU Zheng-ye1, GONG Shui-shui1, GUANG Shan-yi2, KE Fu-you1, XU Hong-yao1*. Determination of the Oxidizability on the Surface of the Graphene Oxide Layer by Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1722-1727. |
[5] |
CAO Hai-qing, HAN Tong-shuai, LIU Xue-yu, LIU Jin*. Extracting Linear Attenuance of Analyte in Turbid Scattering Media and Prediction Model Transfer Based Thereon[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(12): 3711-3717. |
[6] |
FU Sai1, LIN Long-fei1, LIU Yu-ling1, SONG Ji-zheng1, SHAO Jin-xin2, ZHONG Ming3, XIE Tang-gui3, LI Hui1*. Rapid Determination of Water Content in Refined Honey Used as Key Excipient to Form Pills Based on Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(08): 2590-2594. |
[7] |
NIE Li-xing, CHANG Yan, DAI Zhong, MA Shuang-cheng*. A New Method for Determination of Polysorbate 80 in Shengmai Injection Based on Absorption Coefficient[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(01): 199-203. |
[8] |
LIU Yan-de, YE Ling-yu, TANG Tian-yi, OUYANG Ai-guo, SUN Xu-dong, ZHANG Yu. Determination of Performance of Different Concentration Ethanol Diesel Oil Based on Mid Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2741-2748. |
[9] |
SUN Cui-ying, HAN Tong-shuai, GUO Chao, SHENG Wei-nan, LIU Jin*. The Correction Methods for Near Infrared Spectrum of Glucose Aqueous Solution to Reduce the Influence from Temperature[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(11): 3391-3398. |
[10] |
ZHONG Yong-cui1, 3, 4, YANG Li-wei2, QIU Yun-qi2, WANG Shu-mei1,3,4, LIANG Sheng-wang1, 3, 4*. Rapid Determination of Geniposide in Gardenia Jasminoids Ellis in Different Preparations Methods with NIRS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1771-1777. |
[11] |
HU Wen-yan1,3, WANG Yu-heng1,3, ZHANG Xiao-shuan2, LI Jun-hui1,3, WANG Zhong-yi1,3, ZHAO Long-lian1,3*. Determination and 3D Reconstruction of Optical Parameters of Biological Tissue Based on Frequency-Domain Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1374-1379. |
[12] |
OUYANG Ai-guo, HUANG Zhi-hong, LIU Yan-de*. Determination of Methanol Content in Methanol Diesel Based on Near Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1118-1122. |
[13] |
HU Wen-yan1,3, LIU Hui1,3, WANG Yu-heng1,3, LI Jun-hui1,3, ZHANG Xiao-shuan2, WANG Zhong-yi1,3, ZHAO Long-lian1,3*. Study for the Photon Propagation Properties in Biological Tissue Based on Frequency-Domain Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1074-1080. |
[14] |
WANG Zhen-yu, QIU Shu, HE Zheng-bin*, YI Song-lin, MU Jun. Study of Sabina Chinensis Heartwood and Sapwood Pyrolysis with TG-FTIR Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1090-1094. |
[15] |
YU Jing1*, ZHANG Yun1, PANG Song-ying2, WANG Ji-fen2 . Application of IR in the Field of Evidence Identification [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(09): 2807-2811. |
|
|
|
|