|
|
|
|
|
|
Application of Fluorescent Probe Tetraphenyl-1,3-Butadiene in the Detection of Antibiotics |
WANG Rui1, ZHENG Lu-ying1, HU Bo1, ZHANG Xin-yu2, ZHAO Si-si1*, ZHANG Hang1* |
1. College of Chemistry and Chemical Engineering, Shenyang Normal University, Shenyang 110034, China
2. Center for Intelligent and Biomimetic Systems, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
|
|
|
Abstract Antibiotics have the advantages of strong antibacterial activity and low cost and have been widely used in production and daily life. However, the abuse of antibiotics may lead to their accumulation in the human body or food, causing public health problems such as ototoxicity, nephrotoxicity, allergic reactions, and bacterial resistance. Therefore, this article selects fluorescence detection techniques with advantages such as high sensitivity, good selectivity, simple preparation, fast speed, and practical sample detection, which are significant for improving food safety and rational drug use. Based on the application of fluorescence technology in detecting antibiotics, this article selects tetraphenyl-1,3-butadiene (TPB), a typical representative with AIE effect, which has advantages such as stable chemical properties, good luminescence performance and is not limited by solution conditions. It is made into a fluorescence probe to explore the photochemical properties of TPB and the impact of antibiotics on its quenching effect. The research results show that the maximum excitation wavelength of TPB is 365 nm, and the maximum emission wavelength is 435 nm. TPB has strong fluorescence characteristics. At an excitation wavelength of 365 nm, the quantum yield of TPB is 51.9%. When the water volume fraction is 80%, the fluorescence effect of TPB is the best. The fluorescent probe TPB has high selectivity and strong anti-interference ability to tetracycline antibiotics. Tetracycline antibiotics have a high quenching effect on TPB, which meets the condition of fluorescence internal filtering effect, while sulfonamides and quinolones have a poor quenching effect on TPB. The fluorescence intensity of the system decreases with the increase of tetracycline hydrochloride concentration, and there is a strong correlation. The linear regression equation is y=-1.338x+984.20, and the detection limit is 0.042 7 mmol·L-1; When pH≤7, the quenching effect is better; After adding tetracycline hydrochloride solution, the fluorescence intensity of the lake water, sea water and milk system was significantly weakened, and the change of the fluorescence intensity could indirectly reflect the change of the concentration of tetracycline antibiotics solution. This article explores the quenching of TPB by antibiotics, and the detection of antibiotic residues in the environment and food is of great significance, providing a reference for human governance of residual antibiotics in the food and environment.
|
Received: 2023-01-28
Accepted: 2023-04-26
|
|
Corresponding Authors:
ZHAO Si-si, ZHANG Hang
E-mail: zhaoss0905@163.com;zhangh1711@163.com
|
|
[1] Liu P, Zhang Y, Zhang Z Y, et al. Genes, 2023, 14: 1423.
[2] Rusu A, Buta E L. Pharmaceutics, 2021, 13(12): 2085.
[3] Alnassrallah M N, Alzoman N Z, Almomen A. Scientific Reports, 2022, 12(1): 14502.
[4] Xu L S , Wang W Z, Deng J B, et al. Environmental Geochemistry and Health, 2023, 45: 7.
[5] Liu L, Chen Q, Lv J, et al. Inorganic Chemistry, 2022, 61(20): 8015.
[6] Cui M L, Lin Z X, Xie Q F, et al. Food Chemistry, 2023, 412: 135554.
[7] Zhang S, Liu D, Wang G. Molecules, 2022, 27(8): 2586.
[8] Liang J F, Peng C, Li P Y, et al. Bioinorganic Chemistry and Applications, 2023, 5: 100821.
[9] YUE Xiao-yue, LI Yan, ZHOU Zi-jun, et al(岳晓月, 李 妍, 周子君, 等). Journal of Light Industry(轻工学报), 2022, 37(4): 41.
[10] Zhou Y, Mahapatra C, Chen H, et al. Current Opinion in Biomedical Engineering, 2020, 13: 16.
[11] Yang J, Fang M, Li Z. Aggregate, 2020, 1(1): 6.
[12] Jie M S, Lan S K, Lu C R, et al. Journal of Food Measurement and Characterization, 2023, 17: 3173.
[13] Chowdhury P, Banerjee A, Saha B, et al. ACS Biomaterials Science & Engineering, 2022, 8(10): 4207.
[14] HAN Peng-bo, XU He, AN Zhong-fu, et al(韩鹏博, 徐 赫, 安众福, 等). Progress in Chemistry(化学进展), 2022, 34(1): 1.
[15] ZHAO Si-si, ZHANG Xin-yu, QU Hong-yu, et al(赵思思, 张新宇, 曲宏宇, 等). Experimental Technology and Management(实验技术与管理), 2022, 39(5): 148.
[16] Lin X, Wang X L, Li R F, et al. ACS Omega, 2022, 7(13): 10994.
[17] Yuan B, Wang D X, Zhu L N, et al. Chemistry Science, 2019, 10(15): 4220.
[18] Pan X, Lu Y J, Zhou W X, et al. Journal of the Chinese Chemical Society, 2023, 70(3): 737.
[19] Qin W, Alifu N, Lam J W Y, et al. Advanced Materials, 2020, 32(23): 2000364.
[20] Panigrahi S K, Mishra A K. Journal of Photochemistry and Photobiology C: Photochemistry Reviews, 2019, 41: 100318.
[21] Loftin K A, Adams C D, Meyer M T, et al. Journal of Environmental Quality, 2008, 37(2): 378.
[22] Mayr H, Ofial A R. Pure and Applied Chemistry, 2005, 77(11): 1807.
[23] LI Na, LI Zhuo-ran, LIU Cong, et al(李 娜, 李卓然, 刘 聪, 等). Applied Chemical Industry(应用化工), 2015, 44(3): 580.
[24] TANG Kun(唐 琨). Tianjin Science & Technology(天津科技), 2017, 44(7): 56.
[25] FU Hao, LI Xue-bing, WANG Jun(付 浩, 李雪冰, 汪 隽, 等). China Water & Wastewater(中国给水排水), 2017, 33(17): 64.
[26] LUO Kan, SUN Run-tai, SUN Xiao-hong(罗 侃, 孙润泰, 孙晓红, 等). Chinese Journal of Health Laboratory Technology(中国卫生检验杂志), 2006,(9): 1077.
[27] CHENG Jia-xing, ZHAO Qi-yue, LI Ling-jun, et al(程家兴, 赵起越, 李令军, 等). Journal of Instrumental Analysis(分析测试学报), 2018, 37(3): 275.
[28] SUN Hui-jing, LI Pei-wen, ZHANG Bei-bei, et al(孙慧婧, 李佩纹, 张蓓蓓, 等). Chinese Journal of Chromatography(色谱), 2022, 40(4): 333.
|
[1] |
CHENG Wen-xuan1, ZHANG Qing-xian1*, LIU Yu1, ZOU Li-kou2*. Study on Detection of Antibiotic Residues in Eggs by Laser-Induced
Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(05): 1245-1254. |
[2] |
WANG Peng1, 2, 3,WANG Zhen-ya2,WANG Shun2,ZHANG Jie2,ZHANG Zhe2,YANG Tian-hang2,WANG Bi-dou1, 2*,LUO Gang-yin1, 2*,WENG Liang-fei2,ZHANG Chong-yu3,LI Yuan3. Fluorescence Crosstalk Correction for Multiple Quantitative PCR Based on Principal Component Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(04): 1151-1157. |
[3] |
SU Xin-yue1, MA Yan-li2, ZHAI Chen3, LI Yan-lei4, MA Qian-yun1, SUN Jian-feng1, WANG Wen-xiu1*. Research Progress of Surface Enhanced Raman Spectroscopy in Quality and Safety Detection of Liquid Food[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2657-2666. |
[4] |
BAI Jun-peng1, 2, LI Bin1*, ZHANG Shu-juan2, CHEN Yi-mei1. Study on Norfloxacin Concentration Detection Based on Terahertz Time Domain Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2710-2716. |
[5] |
YE Jia-wen1, CHANG Jing-jing1*, GENG Yi-jia2, CUI Yuan1*, XU Shu-ping2, XU Wei-qing2, CHEN Qi-dan3. Detection of I- in Water by the Hg2+@CDs Fluorescent Sensor[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3489-3493. |
[6] |
YANG De-hong1,2, ZHANG Lei-lei1,2, ZHU Cheng1,2*. Application of SERS Technology in the Detection of Harmful Chemical Residues in Agricultural Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3048-3055. |
[7] |
LI Bin, ZHAO Xu-ting, ZHANG Yong-zhen, CHEN Yi-mei. Progress on Terahertz Spectroscopic Detection and Analysis on Antibiotics[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(12): 3659-3666. |
[8] |
MA Li-zhe1, JI Bang2, YANG Zhou2*, HUANG Quan-feng1, ZHAO Wen-feng1*. Study of Photocatalytic Degradation of Antibiotics Based on UV-LED Array[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(09): 2894-2900. |
[9] |
HU Yuan1,2, CHAI Yi-di2, LIU Bo2, WANG Wen-xia2, TANG Jiu-kai2, FU Xin-mei1, WU Jing2*. Properties of Fluorescence Aqueous Fingerprint of Veterinary Antibiotic Wastewater[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3144-3147. |
[10] |
YU Qiang1, CHEN You-peng1,2*, GUO Jin-song1,2. Screening of Antibiotic-Resistant Bacteria in Activated Sludge and Study of Their Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2788-2793. |
[11] |
LIU Huan, LIU Wen, HAN Dong-hai*, WANG Shi-ping*. Three-Dimensional Fluorescence Fingerprint Technique for Milk Quality Evaluation: Antibiotic Residual Detection and Heat-Treated Evaluation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1633-1639. |
[12] |
CHEN Yan-qing, XIE Yu* . Detection of Trace Lead (Ⅱ) with CdZnTe Quantum Dots Capped with Polyethyleneimine as A Fluorescence Probe [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1017-1020. |
[13] |
DONG He, LIU Chuan, DAI Chang-jian* . Study on Raman Spectra of Some Clinical Medicine [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(01): 109-113. |
[14] |
FAN Yu-xia, LAI Ke-qiang, HUANG Yi-qun* . Application of Surface-Enhanced Raman Spectroscopy to the Determination of Trace Chemical Hazards in Food Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(07): 1859-1864. |
[15] |
MA Jun, KONG De-di, HAN Xiao-hong, GUO Wei-li, SHI Xiao-feng . Detection of Antibiotics in Water Using Silver Colloid Films as Substrate of Surface-Enhanced Raman Scattering [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(10): 2688-2693. |
|
|
|
|