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| Study on Detection of Antibiotic Residues in Eggs by Laser-Induced
Fluorescence |
| CHENG Wen-xuan1, ZHANG Qing-xian1*, LIU Yu1, ZOU Li-kou2* |
1. Key Laboratory of Geosciences and Nuclear Technology, Chengdu University of Technology, Chengdu 610059, China
2. College of Resources, Sichuan Agricultural University, Chengdu 611130, China
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Abstract In recent years, the issue of excessive antibiotic residues in the poultry egg industry has drawn considerable attention as a serious food safety concern. Currently, immunoassay, high-performance liquid chromatography, and microbial detection are the three primary techniques used to detect antibiotic residues in livestock and poultry products. When it comes to quick on-site sample testing, these technologies do have certain drawbacks. This study established a quick, accurate, and fast approach for detecting antibiotic residues in eggs using laser-induced fluorescence analysis. The antibiotics that might be found in chicken eggs were chosen for the samples, including ciprofloxacin, norfloxacin, ofloxacin, tetracycline, and gentamicin. The samples were created using egg white as the solvent. Antibiotic reagents were applied to imitate antibiotic residues in eggs, and the fluorescence spectra of antibiotics at various doses were examined. Euclidean distance and probability density approaches were used for the qualitative analysis, which had a 100% accuracy rate within three standard deviations. After examining samples at different concentrations, each antibiotic's calibration curves (c-S curves) were created. The results showed that within the effective detection range (ciprofloxacin: 0.000 1 to 1 mg·mL-1, norfloxacin: 0.000 2 to 1 mg·mL-1, ofloxacin: 0.000 05 to 1 mg·mL-1, tetracycline: 0.000 1 to 0.2 mg·mL-1, chloramphenicol: 0.01 to 1 mg·mL-1), the antibiotic content could be calculated more accurately based on the net peak area of the fluorescence spectra. Ultimately, the precision and detection bound of the antibiotic quantitative curve were computed, suggesting that the technique is applicable to detect antibiotic residues in eggs; however, the high detection limit remains an issue, necessitating additional investigation to lower the detection limit.
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Received: 2023-05-31
Accepted: 2024-01-23
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Corresponding Authors:
ZHANG Qing-xian, ZOU Li-kou
E-mail: shinecore@163.com; zoulikou@sicau.edu.cn
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[1] REN Xing-chao(任兴超). Journal of Anhui Agricultural Sciences(安徽农业科学), 2014, (17): 5465.
[2] JI Shu-hui，QIU Zi-bo(姬书会，邱孜博). The Chinese Livestock and Poultry Breeding(中国畜禽种业), 2022, 18(12): 59.
[3] XIAO Yong-hua，GE Li-ya，LIANG Gao-dao, et al(肖永华，革丽亚，粱高道, 等). Chinese Journal of Food Hygiene(中国食品卫生杂志), 2022, 34(2): 292.
[4] LIU Meng-wen，YANG Huan-huan，ZHAO Yi-peng, et al(刘孟文，杨欢欢，赵一鹏, 等). Modern Preventive Medicine(现代预防医学), 2019, 46(17): 3131.
[5] CHENG Zhao-kang，YANG Jin-shan，LÜ Min, et al(程兆康，杨金山，吕 敏, 等). Journal of Agricultural Resources and Environment(农业资源与环境学报), 2022, 39(6): 1253.
[6] YANG Yao-bin，LIU Shuang，FENG Jia-ying, et al(杨耀彬，刘 爽，冯佳莹, 等). Shandong Chemical Industry(山东化工), 2022, 51(12): 106.
[7] NI Ya-ru，LIU Qi-hua(倪亚茹，刘启华). Journal of Nanjing University of Technology (Social Science)[南京工业大学学报（社会科学版）], 2004, 3(1): 67.
[8] Joseph R Lakowicz. Principles of Fluorescence Spectroscopy. Springer, 2006.
[9] Gutiérrez D, Álvarez J, Racedo F. Journal of Physics. Conference Series, 2019, 1247: 12017.
[10] ZHANG Ling-ling，CHEN Yuan，WANG Cai-xia, et al(张玲玲，陈 媛，王彩霞, 等). Applied Laser(应用激光), 2019, 39(6): 1035.
[11] DU Li-ming，JIN Wei-jun，DONG Chuan, et al(杜黎明，晋卫军，董 川, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001，21(4): 518.
[12] Valeur B, Mário Nuno Berberan-Santos. Molecular Fluorescence: Principles and Applications: Wiley—VCH Verlag GmbH & Co. KGaA, 2012.
[13] Helmut H Telle, Angel González Ureña, Robert J. Donovan. Laser Chemistry: Spectroscopy, Dynamics and Applications. John Wiley & Sons Ltd, 2007.
[14] Crosley D R. Optical Engineering, 1981, 20(4): 511.
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