|
|
|
|
|
|
| Research Progress in Identification and Detection of Fungi Based on SERS Spectroscopy |
| LI Ling1,2, HE Xin-yu1,2, LI Shi-fang1,2, GE Chuang3*, XU Yi1,2,4* |
1. Key Disciplines Laboratory of Novel Micro-nano Devices and System Technology & Key Lab for Optoelectronic Technology & Systems of Ministry of Education, Chongqing 400044, China
2. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China
3. Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing University, Chongqing 400030, China
4. School of Optoelectronics Engineering, Chongqing University, Chongqing 400044, China |
|
|
|
|
Abstract Fungi are a group of pathogenic microorganisms with nuclei and cell walls. They are widespread and cause a variety of diseases in animals, plants, and humans. Fungal infection is one of the most common clinical infectious diseases, making the efficient detection method and related research of fungi attract more attention in recent years. At present, fungi traditional detection methods mainly include culture, microscopy and molecular biology detection, which have the disadvantages of complex operation and time-consuming. Surface-enhanced Raman scattering (SERS) technology has gradually played an important role in fungal detection and identification due to its advantages such as no interference from water, providing molecular fingerprint information and rapid detection. In this paper, based on the brief introduction of the structural characteristics and the common detection methods of fungi, Raman/SERS technology in the identification and detection of fungi was investigated and discussed. Firstly, through the analysis of the characteristics of Raman/SERS and the structure of fungi, according to the related references, the feasibility of SERS technology for fungal detection was analyzed. It was found that there are some problems in the detection of fungi by SERS, such as low sensitivity, complex signal, poor selectivity and specificity, poor signal reproducibility and stability. To solve these problems, the enhancement mechanism of SERS was analyzed. In particular, the review and prospect of the new advances in SERS analysis focused on the nano-enhanced medium materials of SERS, the signal amplification effect of SERS tag and the combination of SERS spectral microfluidic chip analysis technology. The selection of nanomaterials and the construction of nanostructures showed that the SERS enhancement effect produced by the SERS enhancement substrate has great potential in fungal identification and rapid diagnosis of clinical disease. Based on the SERS tag’s signal amplification mechanism, the sensitivity, specificity and reproducibility of fungal SERS detection can be greatly improved. More importantly, the design and integration of SERS nano-enhanced substrates on microfluidic chips and the construction of signal amplification strategy based on SERS tags are more likely to achieve high-throughput and high-content SERS detection of fungal samples, which shows great research value and application prospects in the identification and detection of fungi.
|
|
Received: 2020-06-15
Accepted: 2020-09-26
|
|
|
|
Corresponding Authors:
GE Chuang, XU Yi
E-mail: xuyibbd@cqu.edu.cn；gechuang1115@163.com
|
|
[1] Jamil S, Jamil N, Saad U, et al. JCPSP-Journal of the College of Physicians and Surgeons Pakistan, 2016, 26(2): 113.
[2] Binyam T. Journal of Biology Agriculture & Healthcare, 2015, 5(1): 6.
[3] Sexton D J, Kordalewska M, Bentz M L, et al. Journal of Clinical Microbiology, 2018, 56(12): E01337.
[4] YE Li-yan, WANG Qiang, SHEN Yue-yun, et al（叶丽艳，王 强，沈跃云，等）. Chinese Journal of Laboratory Medicine（中华检验医学杂志）, 2018, 41(8): 577.
[5] Edwards H G M, Russell N C, Weinstein R, et al. Journal of Raman Spectroscopy, 1995, 26(8-9): 911.
[6] Gussem K D, Vandenabeele P, Verbeken A, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2005, 61(13-14): 2896.
[7] Jamieson L E, Asiala S M, Gracie K, et al. Annual Review of Analytical Chemistry, 2017, 10: 415.
[8] Zheng X S, Jahn I J, Weber K, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, 197(SI): 56.
[9] Wang R, Xu Y, Wang C Y, et al. Applied Surface Science, 2015, 349: 805.
[10] Xiang S T, Xu Y, Liao X, et al. Analytical Chemistry, 2018, 90: 13751.
[11] Su X, Xu Y, Zhao H Z, et al. Talanta, 2019, 194: 903.
[12] Li X K, Zhang Y L, Xue B, et al. Biosensors & Bioelectronics, 2017, 92: 517.
[13] Huang Z L, Meng G W, Chen B, et al. Journal of Nanoscience & Nanotechnology, 2016, 16(1): 934.
[14] LIANG Shu-yan, LIU Hong-mei, MU Yun-yun, et al(梁淑妍，刘红梅，穆云云，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2018, 38(1): 87.
[15] He S, Chua J, Tan E K M, et al. RSC Advances, 2017, 7(27): 16264.
[16] Yang B, Jin S, Guo S, et al. ACS Omega, 2019, 4(23): 20101.
[17] Yan S, An R, Zou Y, et al. Sensors and Actuators B: Chemical，2020. 302: 127107.
[18] Qu A H, Wu X L, Xu L G, et al. Nanoscale, 2017, 9(11): 3865.
[19] He Y. Iowa State University, 2015.
[20] Sivanesan A, Witkowska E, Adamkiewicz W, et al. The Analyst, 2014, 139(5): 1037.
[21] Luo Z, Chen L, Liang C. Microchimica Acta, 2017, 184(9): 3505.
[22] Witkowska E, Jagielski T, Kaminska A. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, 192: 285.
[23] Dina N E, Gherman A M, Chis V, et al. Analytical Chemistry, 2018, 90(4): 2484.
[24] Prusinkiewicz M A, Farazkhorasani F, Dynes J J, et al. The Analyst, 2012, 137(21): 4934.
[25] Jabbour R E, Tripathi A, Treado P J, et al. Proceedings of SPIE, 2007, 6554: 65540I-3.
[26] WANG Ke-bing, CHEN Zhi-hong, GUO Bu, et al（王科兵，陈志红，郭 步，等）. Journal of Pharmaceutical Practice（药学实践杂志）, 2017，(5): 422.
[27] Wang Y, Yan B, Chen L. Chemical Reviews, 2013, 113(3): 1391.
[28] Gu X, Trujillo M J, Olson J E, et al. Annual Review of Analytical Chemistry, 2018, 11(1): 147.
[29] Zhao P, Li H X, Li D W, et al. Talanta, 2019, 198: 527.
[30] Bamrungsap S, Treetong A, Apiwat C, et al. Microchimica Acta, 2016, 183(1): 249.
[31] Madiyar F R, Bhana S, Swisher L Z, et al. Nanoscale, 2015, 7(8): 3726.
[32] Mabbott S, Thompson D, Sirimuthu N, et al. Faraday Discussions，2016, 187: 461.
[33] Yuan K, Mei Q, Guo X, et al. Chemical Science, 2019, 10(2): 635.
[34] Zhang C, Wang C, Xiao R, et al. Journal of Materials Chemistry B, 2018, 6: 3751.
[35] Pang Y, Wan N, Shi L, et al. Analytica Chimica Acta, 2019, 1077: 288.
[36] He J, Qiao Y, Zhang H, et al. Biomaterials. 2020, 234: 119763.
[37] Ye Z, Li L L, Tan Z, et al. Applied Surface Science, 2019, 487: 1058.
[38] de Aberasturi D J, Serrano-Montes A B, Langer J, et al. Chemistry of Materials, 2016, 28(18): 6779.
[39] Bohndiek S E, Wagadarikar A, Zavaleta C L, et al. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(30): 12408.
[40] Wu L, Wang Z, Fan K, et al. Small, 2015, 11(23): 2798.
[41] Li Q, Lu Z, Tan X, et al. Biosensors & Bioelectronics, 2017, 97: 59.
[42] Wang L, Li P C H, Yu H Z, et al. Analytica Chimica Acta, 2008, 610(1): 97.
[43] YANG Ning, WANG Pan, ZHANG Rong-biao, et al（杨 宁，王 盼，张荣标，等）. Transactions of the Chinese Society of Agricultural Engineering（农业工程学报）, 2017, 33(20): 161.
[44] Wang C, Madiyar F, Yu C, et al. Journal of Biological Engineering, 2017, 11(1): 9.
[45] Yang D, Zhou H, Dina N E, et al. Royal Society Open Science, 2018，5(9): 180955.
[46] Wang C, Xu Y, Deng C, et al. RSC Advances, 2016, 6(114): 113115. |
| [1] |
LI Qing-jun, SHEN Yan, MENG Qing-hao, WANG Guo-yang, YE Ping, SU Bo*, ZHANG Cun-lin. Terahertz Absorption Characteristics of Potassium Salt Solution Based on Microfluidic Chip[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 363-367. |
| [2] |
ZHAO Xin-yuan, WANG Guo-yang, MENG Qing-hao, ZHANG Feng-xuan, SHAO Si-yu, DING Jing, SU Bo*, ZHANG Cun-lin. Terahertz Transmission Characteristics of Magneto-Fluidic Carrier Liquid Based on Microfluidic Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3012-3016. |
| [3] |
WANG Guo-yang，MENG Qing-hao，SHAO Si-yu，YE Ping，SU Bo*，ZHANG Cun-lin. Terahertz Absorption Characteristics of Low Temperature Liquid Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2709-2713. |
| [4] |
QIAN Kun, BAI Zhi-chen, WU Rui, WANG Jia-hui, SU Bo*, WEN Yi-wei, ZHANG Cun-lin. Terahertz Transmission Characteristics of Electrolyte Solution[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(07): 2018-2022. |
| [5] |
WANG Guo-yang, BAI Zhi-chen, WANG Jia-hui, SU Bo*, ZHANG Cun-lin. Study on Terahertz Absorption Characteristics of Water Based on Microfluidic Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1678-1682. |
| [6] |
CAI Yan, WANG Jia-hui, BAI Zhi-chen, SU Bo*, WU Rui, CUI Hai-lin, ZHANG Cun-lin. Terahertz Transmission Characteristics of Water Induced by Electric Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1683-1687. |
| [7] |
GUO Zhi-ming, YIN Li-mei, SHI Ji-yong, CHEN Quan-sheng, ZOU Xiao-bo. Spectroscopic Techniques for Detection of Mycotoxin in Grains[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1751-1757. |
| [8] |
ZHOU Hao, YANG Zheng. The Reversible Ammonium Detection Based on the Coupled Microfluidic Chipand the Investigation of the Impact Factors[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(12): 3749-3754. |
| [9] |
CAO Xuan1, 2，ZHANG Shu-wei1, 2，CHU Dong-zhi1, 2，WU Ning1, 2，MA Hai-kuan1, 2，MA Ran1, 2. Development of Lab-on-Chip Spectrophotometric Silicate Sensor in situ Analysis of Seawater[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 895-900. |
| [10] |
CAO Xuan1,2, CHU Dong-zhi1,2, LIU Yan1,2*, MA Ran1,2, ZHANG Shu-wei1,2, WU Ning1,2, SHI Qian1,2, MA Hai-kuan1,2. An On-Line Microfluidic Analysis System for Seawater Chemical Oxygen Demand Using Ozone Chemiluminescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3698-3702. |
| [11] |
ZHANG Hao1, GONG Li1, XIE Fang-yan1, ZHANG Wei-hong1, CHEN Qiu-lan2, CHEN Jian1* . Surface Enhanced Spectroscopy Based on Lab-on-a-Chip Technology and Its Applications in Analytical Science [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(02): 350-355. |
| [12] |
Anna Wiejak1, WANG Yuan-zhong2, ZHANG Ji2, Jerzy Falandysz1* . Mercury in Sclerotia of Wolfiporia Extensa (Peck) Ginns Fungus Collected Across of the Yunnan Land[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(09): 3083-3086. |
| [13] |
LI Xiao-li1, LUO Liu-bin1, ZHOU Bin-xiong1, HU Xiao-qian2, SUN Chan-jun1, HE Yong1* . In Vivo Study of Chitin in Fungal Hyphae Based on Confocal Raman Microscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(01): 119-124. |
| [14] |
WANG Shuai1,2, DOU Sen1*, ZHANG Xi2, CUI Yan-jie2, WANG Ting2 . FTIR Spectroscopic Analysis of Humic-Like Substances Extracted from the Microbial Residues [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(12): 3397-3401. |
| [15] |
WANG Xue-mei1, ZHANG Ji2, LI Tao3, LI Jie-qing1, WANG Yuan-zhong2*, LIU Hong-gao1*. ICP-AES Determination of Mineral Content in Boletus Tomentipes Collected from Different Sites of China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(05): 1398-1403. |
|
|
|
|
