| 光谱学与光谱分析 |
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| Identification of Fusarium by Fourier Transform Infrared Spectroscopy |
| NIE Ming1,LUO Jiang-lan1,BAO Kan1,ZHANG Wei-qiong1,YANG Shi-ping1,XIAO Ming1,2*,LI Bo2 |
1. College of Life and Environment Science，Shanghai Normal University，Shanghai 200234，China
2. College of Life Science，Fudan University，Shanghai 200433，China |
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Abstract Fourier transform infrared (FTIR) spectroscopy,in combination with multi-variate statistics,has been a novel tool to identify and classify microorganisms in recent years. In the present study,Fourier transform infrared spectroscopy was used to identify Fusarium belonging to F. oxysporum,F. moniliforme,F. graminearum,F. semitectum,and F. nivale. Distinct spectral differences among these strains were observed from their high-resolution and well-reproducibility infrared spectra in the frequency regions of 3 000-2 800 cm-1,1 800-1 600 cm-1,and fingerprint region. According to the characteristic bands in these regions,different Fusarium species could be identified. And identification by hierarchical cluster analysis was more scientific and reasonable and it also presented a degree of comparability among these strains. But the result was not becoming on reflecting the characteristic of cladistics. FTIR shows high potential as a rapid,accurate and easy-to-use method for the identification of microorganisms. It will be an important tool in microbiological studies.
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Received: 2006-05-26
Accepted: 2006-08-28
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Corresponding Authors:
XIAO Ming
E-mail: xiaoming88@263.net
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| Cite this article: |
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NIE Ming,LUO Jiang-lan,BAO Kan, et al. Identification of Fusarium by Fourier Transform Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1519-1522.
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https://www.gpxygpfx.com/EN/Y2007/V27/I08/1519 |
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