| 光谱学与光谱分析 |
|
|
|
|
|
| Studies on the Action Features between Cefuroxime Axetil and Bovine Serum Albumin |
| WU Gang-ke1,YAN Cheng-nong2*,LIU Yi2,3 |
1. Clinical Laboratory，Central Hospital of Jingzhou，Jingzhou 434020, China
2. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China
3. College of Chemistry and Molecule Sciences, Wuhan University, Wuhan 430072, China |
|
|
|
|
Abstract Under different temperatures and physiological conditions, with cefuroxime axetil concentrations in the range of 1.959×10-6 to 13.71×10-6 mol·L-1, and bovine serum albumin (BSA) concentrations at 2.0×10-6 mol·L-1, the interaction between cefuroxime axetil and BSA was studied by fluorescence spectroscopy, three-dimensional fluorescence spectrum, synchronous fluorescence spectrum and UV-Vis absorption spectroscopy. After analyzing and processing the fluorescence quenching data at different temperatures according to Sterm-Volmer equation，Lineweaver-Burk equation and thermodynamic equation, the average value of the apparent binding constant (KLB: 3.907×106 L·mol-1)，and thermodynamics parameters （enthalpy change ΔH：-13.43 kJ·mol-1, entropy change ΔS：81.90 J·K-1 and standard Gibbs free energy change ΔGθ：-38.34 kJ· mol-1）were calculated, and the amounts of binding sites（n: 1.042）were measured. The fluorescence quenching mechanism of BSA after cefuroxime axetil was added was discussed. BSA was bound with cefuroxime axetil and formed a new compound. The quenching belonged to static fluorescence quenching. The thermodynamic parameters agree with ΔH≈0, ΔS>0 and ΔGθ<0, and the binding reaction is mainly entropy-driven and electro-static interaction force plays a major role in the reaction. The maximum emission wavelength of Tyr and Trp had an obvious red shift in the synchronous fluorescence spectra, the fluorescence emission wavelength of two peaks had a blue shift in the three-dimensional fluorescence spectrum of BSA in the presence of cefuroxime axetil and the maximum absorbtion wavelenghs of three systems in the UV-Vis absorption spectra were obviously different. These showed that the changes in the micro-environment of Tyr and Trp and demonstrated that the conformation of BSA changed as cefuroxime axetil had been added. This provides important information for discussing the configuration modification of BSA because of the added cefuroxime axetil, and for elucidating the pharmacological effects of cefuroxime axetil and biological effects in the organism.
|
|
Received: 2007-05-09
Accepted: 2007-08-19
|
|
|
|
Corresponding Authors:
YAN Cheng-nong
E-mail: yanchn99@163.com
|
|
[1] CHEN Xin-qian, JIN You-yu, TANG Guang(陈新谦, 金有豫, 汤 光). New Medicinal Chemistry(新编药物化学). Beijing：People’s Medical Press(北京：人民卫生出版社)，2003. 60.
[2] PENG Bo, YAN Zheng-yu, SHAO Xiu-fen, et al(彭 博, 严拯宇, 邵秀芬, 等). Chinese Journal of Spectroscopy Laboratory(光谱实验室)，2005，22(5)：913.
[3] BI Shu-yun, DING Lan, SONG Da-qian, et al(毕淑云, 丁 兰, 宋大千, 等). Acta Chimica Sinica(化学学报)，2005，63（23）：2169.
[4] YAN Cheng-nong, SHANGGUAN Yu-feng, LIU Yi, et al(颜承农, 上官云凤, 刘 义, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(9): 1486.
[5] Athina P, Rebecca J G, Richard A F. J. Agric. Food Chem., 2005, 53: 158.
[6] Seetharomappa J, Kamat B P. Chem. Pharm. Bull, 2004, 52: 1053.
[7] Bhalchandra P K. Journal of Pharmaceutical and Biomedical Analysis, 2005, 38: 1046.
[8] YAN Cheng-nong, ZHANG Hua-xun，JU Xiang，et al(颜承农, 张华新, 鞠 香, 等). Chinese Journal Analytical Chemistry(分析化学)，2005，33（6）：759.
[9] YANG Pin, GAO Fei(杨 频, 高 飞). Principle of Bioinorganic Chemistry(生物无机化学原理). Beijing：Science Press(北京：科学出版社)，2005. 57.
[10] YANG Man-man, YANG Pin, ZHANG Li-wei(杨曼曼, 杨 频, 张立伟). Chinese Science Bulltin(科学通报)，1994，39：31.
[11] ZHANG Bao-lin, WANG Wen-qing, BAI Feng-lian(张宝林, 王文清, 白凤莲). Chemical Journal of Chinese Universities(高等学校化学学报), 1994, 15：373
[12] Mauricios B, Guilherme L I. J. Phys. Chem. B，1998, 102：4678.
[13] Ross D P, Subramanian S. Biochemistry, 1981, 20: 3096.
[14] Nemethy G，Scheraga H A. J. Phys. Chem., 1962，66: 1773.
[15] Timasheff S V，Peeters H(ED). Protein of Biological Fluids，Oxford：Pergamon Press，1972. 511.
[16] Frster T. Modern Quantum Chemistry, New York：Academic Press, 1996. 93.
[17] YAN Cheng-nong, MEI Ping, XIONG Dan, et al(颜承农, 梅 平, 熊 丹, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(12): 2306.
[18] ZHANG Hai-rong, GUO Si-yuan, LI Lin, et al(张海容, 郭祀远, 李 琳，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001，21（6）：829.
[19] YAN Yuan, XU Jin-gou, CHEN Guo-zhen(鄢 远, 许金钩, 陈国珍). Science in China(Series B)(中国科学B辑)，1997, 27：16.
[20] YAN Cheng-nong, ZHANG Hua-xin, MEI Ping, et al(颜承农, 张华新, 梅 平, 等). Chinese Journal Chemistry(中国化学)，2005，23：1151.
|
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [4] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [5] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [6] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [7] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [8] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [9] |
QIU Cun-pu1, 2, TANG Xiao-xue2, WEN Xi-xian4, MA Xin-ling2, 3, XIA Ming-ming2, 3, LI Zhong-pei2, 3, WU Meng2, 3, LI Gui-long2, 3, LIU Kai2, 3, LIU Kai-li4, LIU Ming2, 3*. Effects of Calcium Salts on the Decomposition Process of Straw and the Characteristics of Three-Dimensional Excitation-Emission Matrices of the Dissolved Organic Matter in Decomposition Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2301-2307. |
| [10] |
SHI Chuan-qi1, LI Yan2, HU Yu3, YU Shao-peng1*, JIN Liang2, CHEN Mei-ru1. Fluorescence Spectral Characteristics of Soil Dissolved Organic Matter in the River Wetland of Northern Cold Region, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1517-1523. |
| [11] |
LI Yuan-jing1, 2, CHEN Cai-yun-fei1, 2, LI Li-ping1, 2*. Spectroscopy Study of γ-Ray Irradiated Gray Akoya Pearls[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1056-1062. |
| [12] |
LIU Xia-yan1, CAO Hao-xuan1, MIAO Chuang-he1, LI Li-jun2, ZHOU Hu1, LÜ Yi-zhong1*. Three-Dimensional Fluorescence Spectra of Dissolved Organic Matter in Fluvo-Aquic Soil Profile Under Long-Term Composting Treatment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 674-684. |
| [13] |
LI Jin-zhi1, LIU Chang-jin1, 4*, SHE Zhi-yu2, ZHOU Biao2, XIE Zhi-yong2, ZHANG Jun-bing3, JIANG Shen-hua2, 4*. Antiglycation Activity on LDL of Clove Essential Oil and the Interaction of Its Most Abundant Component—Eugenol With Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 324-332. |
| [14] |
LÜ Yang1, PEI Jing-cheng1*, ZHANG Yu-yang2. Chemical Composition and Spectra Characteristics of Hydrothermal Synthetic Sapphire[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3546-3551. |
| [15] |
ZHANG Yong-bin1, ZHU Dan-dan1, CHEN Ying1*, LIU Zhe1, DUAN Wei-liang1, LI Shao-hua2. Wavelength Selection Method of Algal Fluorescence Spectrum Based on Convex Point Extraction From Feature Region[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3031-3038. |
|
|
|
|
