|
|
|
|
|
|
| The Application of Confocal Raman Spectroscopy in Mussels Shell |
| CUI Nan-nan1, 3, 4, DU Zeng-feng1, 4, ZHANG Xin1, 2, 3, 4*, LUAN Zhen-dong1, 4, XI Shi-chuan1, 3, 4, LI Lian-fu1, 3, 4, WANG Min-xiao1, 4, WANG Bing1, 4, LIANG Zheng-wei1, 3, 4, LIU Jing1, 3, 4, LIAN Chao1, 4, YAN Jun1, 4 |
1. CAS Key Laboratory of Marine Geology and Environment & Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2. Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, China
3. University of Chinese Academy of Sciences, Beijing 100049, China
4. Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China |
|
|
|
|
Abstract Mussels are widely distributed in global waters, from offshore shallow waters to hydrothermal vents and cold seeps in the deep oceans. Mussels secrete calcium carbonate and form shells to protect their soft tissues. The nancomposite properties of mussel shell and the application prospects in biomaterial, tissue engineering and bionics have attracted much attention of scientists. The Raman spectroscopy is a kind of non-destructive, non-contact, simultaneous multi-component analysis detection technique, and can provide mineral information of samples. The mineral composition and distribution patterns of the shells and the nacre layers of the mussels collected from four different study sites (cold seeps in the Southwest of Taiwan, Desmos hydrothermal Field, Laboratory rearing, and Dalian-offshore) were obtained using the confocal Raman technique. The results showed that both of the prismatic layer and the nacre layers of the mussels are calcium carbonate. The Raman shifts of the primatic layers locate at 711 and 281 cm-1 indicating the mineral composition of prismatic layer is calcite. The mineral composition of nacre exists difference in different environment although the shell nacre is mainly calcium carbonate: the mineral of nacre in Dalian-offshore is aragonite. The Raman shifts of aragonite are at 706 and 206 cm-1. Aragonite crystallizes poor. The mineral of nacre layers grown in hydrothermal field and cultured in laboratory are aragonite (706 and 206 cm-1) with good crystallization. The mineral of mussel nacre in cold seep is aragonite (706 and 206 cm-1) and contains a little calcite. The Raman shifts of calcite are at 711 and 281 cm-1. Taking the living environment conditions into consideration, the results suggested that difference in the mineral composition and distribution patterns in the mussel shells are probably caused by different physical and chemical properties within the four environments. Moreover the nacre layers is more sensitive to the pressure change of living environment. Our work also showed that the Raman spectroscopy is a quick and effective technique that can be used to analyze the mineral composition of mussels in different environments and may shine some lights on the study of the life processes and adaptive mechanisms of mussels in deep oceans.
|
|
Received: 2019-01-25
Accepted: 2019-04-22
|
|
|
|
Corresponding Authors:
ZHANG Xin
E-mail: xzhang@qdio.ac.cn
|
|
[1] Ricardo F G, Elena M C, Carmen G B, et al. Environmental Science & Pollution Research, 2011，18(7): 1139.
[2] Griffith E，Paytan A. Sedimentology, 2012，59(6): 1817.
[3] Antje H, Christian R, et al. Frontiers in Microbiology, 2012，3: 253.
[4] Sarrazin J, Cuvelier D, Peton L, et al. Deep Sea Research Part I Oceanographic Research Papers, 2014，90(7): 62.
[5] XI Shi-chuan, ZHANG Xin, DU Zeng-feng, et al(席世川，张 鑫，杜增丰, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2018，38(11)：3390.
[6] Smaal A C, Schellekens T, van Stralen M R, et al. Aquaculture, 2013. 404-405: 28.
[7] Scurr D J，Eichhorn S J. Journal of Materials Research, 2006，21(12): 3099.
[8] Masic D, Harrington M, Waite J H, et al. AIP Conference Proceedings, 2010, 1267: 358.
[9] Nehrke G, Poigner H, Wilhelms-Dick D. Geochem. Geophys. Geosyst., 2012, 13: Q05014.
[10] Li Shiguo, Liu Chuang, Huang Jingliang, et al. Journal of Experimental Biology, 2015，218(22): 3623.
[11] Álvarezdocio C M, Reinosa J J, Campo A, et al. Dyes & Pigments, 2017，137: 1.
[12] Ševčík R，Mácová P. Vibrational Spectroscopy, 2018，95: 1. |
| [1] |
WANG Mei-li1, 2, SHI Guang-hai2*, ZHANG Xiao-hui1, YANG Ze-yu2, 3, XING Ying-mei1. Experimental Study on High-Temperature Phase Transformation of Calcite[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1205-1211. |
| [2] |
LIANG Zheng-wei1, 3, 4, DU Zeng-feng1, 4, LI Chao-lun1, 3, 4, WANG Min-xiao1, 3, 4, WANG Bing1, 4, ZHANG Xin1, 2, 3, 4*, YAN Jun1. Confocal Raman Micro-Spectroscopy Analysis of Mussel Foot[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(03): 755-759. |
| [3] |
FU Wan-lu1, YUAN Xue-yin2*. Study on the Influence of Magnesium on the Phase Transition Pressures and Raman Vibrations of Calcite[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2053-2058. |
| [4] |
ZENG Chang-yu1, 4, ZHAO Ming-zhen2, LI Hong-zhong3, 4*, NIU Jia1, 4, ZHANG Jie-tang1, 4, HE Jun-guo1, 4*, ZHOU Yong-zhang1, 4, YANG Zhi-jun1, 4. Feieling Skarn-Type Pb-Zn Deposit in Southwest Margin of Yunkai Massif[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(09): 2558-2562. |
| [5] |
ZHANG Xuan1, HU Chao1, YAN Yan1, YANG Hai-feng1, LI Jun-fang1, BAI Hua1, XI Guang-cheng1*, LIAO Jie2 . Identification of Pearl Powder Using Microscopic Infrared Reflectance Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(09): 2424-2428. |
| [6] |
YAN Jun1, 2, TAO Jin-bo2, DENG Xiao-qiong2, HU Xian-chao3, WANG Xiao-xiang1* . The Unique Reflection Spectra and IR Characteristics of Golden-Color Seawater Cultured Pearl [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(05): 1206-1210. |
| [7] |
LI Hong-zhong1, ZHAI Ming-guo1, ZHANG Lian-chang1, YANG Zhi-jun2, 3*, ZHOU Yong-zhang2, 3, WANG Chang-le1, LIANG Jin2, 3, LUO An2. Study on Microarea Characteristics of Calcite in Archaean BIF from Wuyang Area in South Margin of North China Craton and Its Geological Significances[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(11): 3061-3065. |
| [8] |
FAN Lu-wei1*, ZHANG Yan2, HU Yang3 . Vibrational Spectra of Corallium Elatius [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(09): 2329-2331. |
| [9] |
FU Pei-ge, ZHENG Hai-fei* . Raman Spectra of Aragonite and Calcite at High Temperature and High Pressure [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(06): 1557-1561. |
| [10] |
XIE Chao1, 2, ZHOU Ben-gang1*, DU Jian-guo2, YI Li2, CHEN Zheng-wei2 . Characteristics of Raman Spectra of Minerals in Gouge of the Wenchuan Earthquake Fault Zone [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(06): 1562-1565. |
| [11] |
LIU Chuan-jiang, ZHENG Hai-fei*. In Situ Experimental Study of Phase Transition of Calcite by Raman Spectroscopy at High Temperature and High Pressure [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(02): 378-382. |
| [12] |
WANG Shi-xia1,2, ZHENG Hai-fei2* . Research on Raman Spectra of Calcite Phase Transition at High Pressure [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(08): 2117-2119. |
| [13] |
FU Pei-ge, ZHENG Hai-fei* . Raman Spectra of Aragonite at the Pressure of 0.1~2 GPa and Ambient Temperature [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(01): 127-130. |
| [14] |
GAO Yong-hua, LI Zhuo, QIAO Li, REN Dong-ni, FENG Qing-ling*. Raman and FTIR Characteristics of Otolith of Ornamental Carp[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(10): 2689-2693. |
| [15] |
JIA Tai-xuan1,2,LIU Zi-li1,2*,ZHANG Gang-sheng1. XRD and FTIR Spectra Characteristics of Nacreous Layer in Perna Viridis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(01): 131-133. |
|
|
|
|
