荧光光谱技术的交趾黄檀和奥氏黄檀树种识别新方法

doi:10.3964/j.issn.1000-0593(2019)07-2182-08

Abstract
References
Related Citation (6)

Download: PDF (6559 KB)
Export: BibTeX | EndNote (RIS)

Abstract The fluorescence characteristic of small molecular organic compound in nature has potential application value for plant species identification. At present, the identification of two species about Dalbergia cochinchinensis Pierre and Dalbergia oliveri Prain based on the anatomical characteristics of wood has its limitation. So, we explored a new method for identification of Dalbergia cochinchinensis Pierre and Dalbergia oliveri Prain by fluorescence spectroscopy in the paper. The fluorescent substances were completely extracted in five different wood samples of Dalbergia cochinchinensis Pierre and Dalbergia oliveri Prain. Fluorescence photos, two-dimensional and three-dimensional fluorescence spectrum were used to confirm the species and quantity of fluorescent substances in wood samples of the two species. The results of fluorescence photograph shown that the extract solutions of Dalbergia cochinchinensis Pierre and Dalbergia oliveri Prain both present obvious fluorescence characteristics and the fluorescence substances are stable in two years of storage. Two-dimensional and three-dimensional fluorescence spectrum exhibit the fluorescence characteristics of the two species in more detail. The results indicate that there is a fluorescent substance in the extract solution of Dalbergia cochinchinensis Pierre, and the maximum excitation and emission wavelength of the fluorescence substance are 297 and 327 nm, respectively. However, the fluorescence spectrum of extract solution from Dalbergia oliveri Prain present two maximal excitation and emission wavelengths respectively, the maximum excitation is 317 and 333 nm, and the maximum emission is 342 and 358 nm, which indicates that there are two kinds of fluorescent substances or one kind of fluorescence substance containing two groups of fluorescence. The respective quantity and characteristics of fluorescent substances of the two species are obviously accordant, and there are obvious differences between the two species, which can be used to confirm their identities. Moreover, the extracting methods of fluorescent substances from the wood sample of the two species are built in this paper. Acetonitrile is an optimal reagent for the extraction of fluorescent substance in the wood sample of Dalbergia cochinchinensis Pierre, and the mixed reagent prepared according to the volume ratio of ethyl acetate and acetonitrile equals 10∶1 is more suitable for that of fluorescent substance in Dalbergia oliveri Prain. The extraction of fluorescent substance is beginning to level off within 12 hours for the wood samples of Dalbergia cochinchinensis Pierre and Dalbergia oliveri Prain. The results indicate that the fluorescence spectroscopy technique can be used to identify Dalbergia cochinchinensis Pierre and Dalbergia oliveri Prain. The method will be of very important value to the identification of rosewood species.

Key words：Fluorescent spectroscopy; Dalbergia cochinchinensis Pierre; Dalbergia oliveri Prain; New method for rosewood identification

Received: 2018-05-31 Accepted: 2018-10-12

ZTFLH:

O657.3

Cite this article:

URL:

https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2019)07-2182-08 OR https://www.gpxygpfx.com/EN/Y2019/V39/I07/2182

[1] CITES, 2017. Convention on International Trade in Endangered Species of Wild Fauna and Flora, Appendices Ⅰ, Ⅱ and Ⅲ. UNEP-WCMC.
[2] Barrett M A, Brown J L, Yoder A D. Nature, 2013, 499: 29.
[3] Gasson P, Miller R, Stekel D J, et al. Annals of Botany, 2010, 105: 45.
[4] Ugochukwu A I, Hobbs J E, Phillips P W B, et al. Ecological Economics, 2018, 146: 730.
[5] FU Jian-guo, LIU Jin-liang, YANG Xiao-jun, et al(伏建国，刘金良，杨晓军，等). Journal of Zhejiang A&F University(浙江农林大学学报), 2013, 30(3)：438.
[6] Kang Yong, Deng Zhiyan, Zang Runguo, et al. Scientific Reports, 2017, 7: 12564.
[7] GAO Lian-ming, LIU Jie, CAI Jie, et al(高连明，刘杰，蔡杰，等). Plant Diversity and Resources(植物分类与资源学报), 2012, 34(6)：592.
[8] Dong W P, Xu C, Li C H, et al. Scientific Reports, 2015, 5: 8348.
[9] Gasson P. IAWA Journal, 2011, 32(2)：137.
[10] Jiao L C, Yin Y F, Xiao F M, et al. IAWA Journal, 2012, 33(4): 441.
[11] ZHANG Rong, YIN Ya-fang, XU Kui-wu, et al(张蓉，殷亚方，徐魁梧，等). Journal of Nanjing Forestry University·Natural Sciences Edition(南京林业大学学报·自然科学版), 2014, 38(1)：151.
[12] Huang A M, Zhou Q, Liu J L, et al. Journal of Molecular Structure, 2008, 883：160.
[13] SHEN Ming-yue, ZHANG Qi-yuan, ZHU Zhong-liang, et al(沈明月，章启元，朱仲良，等). Science Silvae Sinicae(林业科学), 2012, 48(5)：168.
[14] Silva Guzmán J A, Richter H G, Anda R R, et al. IAWA Journal, 2008, 29(3)：311.
[15] Li Q F, Wang J T, Wu S Y, et al. Sensors and Actuators B: Chemical, 2018, 259：484.
[16] Masakazu M, Yuta T, Karina H. Dyes and Pigments, 2018, 153：144.
[17] Joseph R Lakowicz. Principles of Fluorescence Spectroscopy(Third Edition), Springer, 2006.
[18] LUO Bei, HE Rui, YANG Yan(罗蓓，何蕊，杨燕). Journal of Beijing Forestry University(北京林业大学学报), 2018, 40(1)：120.