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Design and Application of Small NIR-Raman Spectrometer Based on Dichroic and Transmission Collimating |
GAO Hao1, WANG Xiao1, SHANG Lin-wei1, ZHAO Yuan1, YIN Jian-hua1*, HUANG Bao-kun2* |
1. Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China
2. Huaihai Institute of Technology, Lianyungang 222005, China |
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Abstract In order to meet professional requirements for the portable and high-performance Raman spectrometer, a small near-infrared (NIR) Raman spectrometer for biomedical detection was built in this work. In addition, assembly system was completed through the theoretical calculation, geometric optical path design. Differences from the traditional structure with reflective collimation were shown as below. (1) Transmission-based collimation method was adopted in dispersion system of the spectrometer, so the collimated scattering light projected onto the grating for dispersion. (2) Following dichroic mirror for reflection and penetration, objective lens converging the incident light and collecting the scattered light, the Raman spectrometer was designed to meet confocal plane at the slit between the collection unit and dispersion system, which is useful to collect the Raman signal and remove the stray light. (3) The spectrometer system achieves the high-resolution (3 cm-1), high repeatability and high sensitivity for spectral detection ranging of 500~2 200 cm-1 (785 excitation). (4) The entire optical system was designed in the size of ca. 240 mm×200 mm×130 mm, therefore achieving miniaturization for this NIR Raman spectrometer and flexible assembly of components. It was then used to measure Raman spectra of glucose and knee cartilage and get excellent results by comparing with that obtained by huge commercial Raman spectrometer. The results show that the spectrometer has high resolution, high reproducibility and high sensitivity, etc, thus it can be flexibly applied in biomedical and other research fields.
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Received: 2017-07-03
Accepted: 2018-01-15
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Corresponding Authors:
YIN Jian-hua, HUANG Bao-kun
E-mail: yin@nuaa.edu.cn;huang_baokun@163.com
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[1] Zhu Xiaoqin, Xu Tao, Lin Qingyu, et al. Applied Spectroscopy Reviews, 2014, 49(1): 64.
[2] Das R S, Agrawal Y K. Vibrational Spectroscopy, 2011, 57(2): 163.
[3] Zhang G D, Lin H. Applied Mechanics & Materials, 2013, 241-244: 449.
[4] Shafer A B, Megill L R, Droppleman L A. Journal of the Optical Society of America, 1964, 54(7): 879.
[5] Lee K S, Thompson K P, Rolland J P. Optics Express, 2010, 18(22): 23378.
[6] Xue Q. Applied Optics, 2011, 50(10): 1338.
[7] AN Yan, SUN Qiang, LIU Ying,et al(安 岩, 孙 强, 刘 英, 等). Chinese Optics(中国光学), 2012, 5(5): 470.
[8] Somerville W R C, Ru E C L, Northcote P T, et al. American Journal of Physics, 2010, 78(7): 671.
[9] Tromberg B J, Shah N, Lanning R, et al. Neoplasia, 2000, 2(1-2): 26.
[10] Movasaghi Z, Rehman S, Rehman I U. Applied Spectroscopy Reviews, 2007, 42(5): 493.
[11] LIU Ke-dian(刘可滇). Analytical Instrumentation(分析仪器), 2009,(4): 22.
[12] LI Can, HUANG Bao-kun, FENG Zhao-chi, et al(李 灿,黄保坤,冯兆池,等). Chinese Patent(中国专利),104422681A,2015.
[13] LI Quan-chen, JIANG Yue-juan(李全臣,蒋月娟). Principles of Spectral Instruments(光谱仪器原理). Beijing: Beijing Institute of Technology Press(北京:北京理工大学出版社),1999. 102.
[14] WANG Bo,GONG Sen,CAI Zhi-peng, et al(王 波, 龚 森, 蔡志鹏,等). Journal of Light Scattering(光散射学报), 2009, 21(1): 18.
[15] Esmonde-White K A, Esmonde-White F W L, Morris M D, et al. Analyst, 2011, 136(8): 1675.
[16] Vardaki M Z, Papachristou D J, Megas P, et al. Bone,2012, 50: S190.
[17] Buchwald T, Niciejewski K, Kozielski M, et al. Journal of Biomedical Optics, 2012, 17(1): 017007. |
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