|
|
|
|
|
|
| The Improved Method of Trace Content for Raman Measurement Accuracy |
| ZHAO Xiao-yu1，ZHAI Zhe2，TAN Feng1，TONG Liang3，TIAN Fang-ming1，LIU Chang1 |
1. College of Electrical and Information, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2. Chinese Academy of Forestry, Beijing 102300, China
3. Communication and Electronic Engineering Institute, Qiqihar University, Qiqihar 161006, China |
|
|
|
|
Abstract To improve the measurement accuracy for the Raman spectrum of micro-substance is one of the difficulties in Raman analysis technique, which is prominent when measuring the micro-substance in organism under the background of high fluorescence. According to the burst and dispersed characteristics to Raman spectrum peak, the fluorescence background and noise fitting function was given in this paper respectively. The location of Raman spectrum peak and interference signal filtering had been realized through monitoring A-type uncertain function in the overall fitting deviation. The spectrum section had been divided according to the spectrum peak’s location further, and the spectrum peak signal had been enhanced through weakening the non-spectrum peak signal within monotone interval. Compared to other fluorescent processing methods, this method could split the overlapped characteristic peak accurately, however, the height of the characteristic peak will not be lowered and more sensitive semi-spectrum peak index could be offered. The experiment indicates that while processing the skin Raman spectrum, such method could obtain the affiliation Raman spectra of the amide I-belt, ceramide and CO ester in helical conformation. What’s more, the measurement model with the content of water-soluble sugar (rice leaf) had been established with the data after being processed by such method, and it had been found that the precision is superior to that of the model established by using the processing data of wavelet decomposition, fitting of a polynomial and nonlinear least square method.
|
|
Received: 2017-04-01
Accepted: 2017-08-31
|
|
|
|
[1] Schulze G, Jirasek A, Yu M M L. Appl. Spectrosc., 2005, 59: 545.
[2] O’Grady A, Dennis A C, Denvir D. Anal. Chem.，2001, 73：2058.
[3] Zhang D M, Ben-Amotz D. Appl. Spectrosc.，2000, 54：1379.
[4] Mosierboss P A，Lieberman S H, Newbery R. Appl. Spectrosc.，1995, 49：630.
[5] Mahadevan-Jansen A, Mitchell M F, Ramanujam N. Photochem. Photobiol.，1998, 68：123.
[6] Zhao J, Lui H, Mclean D I. Appl. Spectrosc.，2007, 61：1225.
[7] Brennan J F, Wang Y, Dasari R R. Appl. Spectrosc.，1997, 51：201.
[8] Cai T T, Zhang D M, Ben-Amotz D. Appl. Spectrosc.，2001, 55：1124.
[9] Caspers P J, Lucassen G W, Wolthuis R. Biospectroscopy，1998, 4(45): 31.
[10] Nicholas Stone, Catherine Kendall, Jenny Smith. Faraday Discuss, 2004, 126: 141.
[11] Wood B R, Tait B, McNaughton D. BBA-Molecular Cell Research, 2001, 1539: 58.
[12] Xie Chan-gan, Li Yong-qing, Tang Wei，et al. J. Appl. Phys., 2003, 94(9): 6138. |
| [1] |
XIAO Hu-ying1, YANG Fan1, XIANG Liu1, HU Xue-jiao2*. Jet Vacuum Enhanced Tunable Diode Laser Absorption Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 2993-2997. |
| [2] |
ZHU Ze-zhong1，2, SHEN Hua1，2*, WANG Nian1，2, ZHU Ri-hong1，2. Transient Measure Technique for Excitation Temperature and Radiation Temperature Based on Multi-Spectral Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 333-339. |
| [3] |
ZHU Ze-zhong1，2, SHEN Hua1，2*, WANG Nian1，2, ZHU Ri-hong1，2 . Radiation Temperature Measurement Technology Based on the Basis of Spectral Emissivity Function [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(03): 685-691. |
| [4] |
YU Jie1, HONG Xu1, MA Ying-jie1*, ZHOU Jian-bin1, WANG Yi-qiang2, WANG Min1, LU Yuan-sheng1. Study on Heavy Metal Detection in Soil with Improved EDXRF [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(10): 3429-3433. |
| [5] |
WANG Feng-fei1，2, LUO A-li1, ZHAO Yong-heng1 . The Radial Velocity Measurement Accuracy of Different Spectral Type Low Resolution Stellar Spectra at Different Signal-to-Noise Ratio [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(02): 565-568. |
| [6] |
DU Wei1,2,3, LUO A-li1,2, ZHAO Yong-heng1,2 . Effects of Spectral Resolution and Noise on the Measurement of Indices [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(07): 1886-1890. |
| [7] |
LI Gang, LI Qiu-xia, LIN Ling, LI Xiao-xia, WANG Yan, LIU Yu-liang. Discussion about the Prediction Accuracy for Dynamic Spectrum by Partial FFT [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2177-2180. |
|
|
|
|
