|
|
|
|
|
|
Exploration of the Interaction Mechanism between Doxorubicin Hydrochloride and DNA by Spectroscopic Techniques and Isothermal Titration Calorimetry |
WANG Huan, GOU Xing-xing, PU Xiao-hua*, WANG Jiao, HU Xiao-bing, LI Zong-xiao |
College of Chemistry and Chemical Engineering, Baoji University of Arts and Sciences, Baoji 721013, China |
|
|
Abstract The interaction processing of anti-cancer drug doxorubicin hydrochloride (DOX) with DNA was systematically investigated using fluorescence spectroscopy, ultraviolet-visible spectroscopy, infrared spectroscopy,circular dichroism and isothermal titration calorimetry measurements. The binding constant Ka, the binding-site number n, the reaction enthalpy ΔH and the reaction entropy ΔS of the interaction were obtained. In the binding process, the helix of B-DNA can have a certain degree of structural variations. The data of fluorescence spectrum indicated that DNA was a good quencher for the fluorescence of DOX. IR data certified the cation DOX+ binding with the phosphate groups of DNA through strong electrostatic attraction, while the C—H bonds of DOX may be associated with the bases of DNA by hydrophobic interaction. The Changes of enthalpy and entropy of the binding process were determined by ITC, which indicated that the process was driven by the hydrophobic interactions between the hydrocarbon chain of DOX and the bases of DNA.
|
Received: 2016-07-02
Accepted: 2016-12-28
|
|
Corresponding Authors:
PU Xiao-hua
E-mail: pxh913@163.com
|
|
[1] Doyle M L. Curr. Opin. Biotech.,1997, 8(1): 31.
[2] Nguyen B, Hamelberg D, Bailly C, et al. Biophys. J,2004, 86(2): 1028.
[3] Pitha J, Smid J. BBA-Biomembranes,1976, 425(3): 287.
[4] Luck A N, Mason A B. Adv. Drug. Deliver. Rev., 2013, 65(8): 1012.
[5] Rauf S, Gooding J, Akhtar K, et al. J. Pharmaceut. Biomed.,2005, 37(2): 205.
[6] Whittaker J, McFadyen W D, Baguley B C. Anti-Cancer. Drug. Des.,2001, 16(2): 81.
[7] Yarnell A T, Oh S, Reinberg D, et al. J. Biol. Chem.,2001, 276(28): 25736.
[8] Zhang J, Jian Y, Jun G, et al. Cell Res.,2004, 14(4): 283.
[9] Nishimura T, Okobira T, Kelly A M, et al. Biochemistry,2007, 46(27): 8156.
[10] Ding Y, Zhang L, Xie J, et al. J. Phys. Chem. B,2010, 114(5): 2033.
[11] Pérez-Arnaiz C, Busto N, Leal J M, et al. J. Phys. Chem. B,2014, 118(5): 1288.
[12] Chen P, Qiu M, Deng C, et al. Biomacromolecules,2015, 16(4): 1322.
[13] Johnson R P, Uthaman S, John J V, et al. Applied Materials & Interfaces,2015, 7(39): 21770.
[14] Tavano L, Muzzalupo R, Mauro L, et al. Langmuir.,2013, 29(41): 12638.
[15] Zunino F, Gambetta R, Di Marco A, et al. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis,1977, 476(1): 38.
[16] Pyle A, Rehmann J, Meshoyrer R, et al. J. Am. Chem. Soc.,1989, 111(8): 3051.
[17] Cohen G, Eisenberg H. Biopolymers.,1969, 8(1): 45.
[18] Jangir D K, Tyagi G, Mehrotra R, et al. J. Mol. Struct.,2010, 969(1): 126.
[19] LIU Xiang-rong, SUN Xiu-chao, YANG Zai-wen, et al(刘向荣,孙秀超,杨再问,等). Chinese J. Inorg. Chem.(无机化学学报),2016, 32(2): 250.
[20] Ware W R. J. Phys. Chem. B,1962, 66(3): 455.
[21] Bi S, Song D, Kan Y, et al. Spectrochim. Acta A,2005, 62(1): 203.
[22] WANG Hui, GAN Guo-qing, QU Yang, et al(王 慧,甘国庆,瞿 阳,等). Chinese. J. Inorg. Chem.(无机化学学报),2012, 28(6): 1217.
[23] Raja D S, Bhuvanesh N S P, Natarajan K. Inorganica. Chimica Acta,2012, 385: 81.
[24] Fotticchia I, Fotticchia T, Mattia C A, et al. Langmuir.,2014, 30(48): 14427.
[25] Grüner S, Neeb M, Barandun L J, et al. BBA-Gen Subjects,2014, 1840(9): 2843.
[26] Jelesarov I, Bosshard H R. J. Mol. Recognit.,1999, 12(1): 3. |
[1] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[2] |
YE Zi-yi, LIU Shuang, ZHANG Xin-feng*. Screening of DNA Dyes for Colorimetric Sensing Via Rapidly Inducing Gold Nanoparticles Aggregation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2805-2810. |
[3] |
WU Lei1, LI Ling-yun2, PENG Yong-zhen1*. Rapid Determination of Trace Elements in Water by Total Reflection
X-Ray Fluorescence Spectrometry Using Direct Sampling[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 990-996. |
[4] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, YUE Yuan-bo2, HU Xue-qiang2, CHEN Yu2, LI Xiao-jia1, 2*. The Detection of Mercury in Solutions After Thermal Desorption-
Enrichment by Energy Dispersive X-Ray Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1117-1121. |
[5] |
WEI Yi-hua1, HUANG Qing-qing2, ZHANG Jin-yan1*, QIU Su-yan1, 3, TU Tian-hua1, YUAN Lin-feng1, DAI Ting-can1, ZHANG Biao-jin1, LI Wei-hong1, YAN Han1. Determination of 5 Kinds of Selenium Species in Livestock and Poultry Meat With Ion Pair Reversed Phase Liquid Chromatography-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3822-3827. |
[6] |
LIU Yu1, LI Zeng-wei2, DENG Zhi-peng1, ZHANG Qing-xian1*, ZOU Li-kou2*. Fast Detection of Foodborne Pathogenic Bacteria by Laser-Induced Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2817-2822. |
[7] |
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372. |
[8] |
NI Zi-yue1, CHENG Da-wei2, LIU Ming-bo2, HU Xue-qiang2, LIAO Xue-liang2, YUE Yuan-bo2, LI Xiao-jia1,2, CHEN Ji-wen3. The Rapid Detection of Trace Mercury in Soil With EDXRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 734-738. |
[9] |
LIU Chong-hua, OUYANG Yu, CHEN Guan-qian, PENG Cai-hong, SONG Wu-yuan. Rapid Determination of Chromium, Arsenic, Selenium, Cadmium, Antimony, Barium, Mercury and Lead in Toy Plastics by Energy Dispersive X-Ray Fluorescence Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 739-744. |
[10] |
ZHAO Ting1,2,3, CHI Hai-tao1,2,3*, LIU Yi-ren1,2,3, GAO Xia1,2,3, HUANG Zhao1,2,3, ZHANG Mei1,2,3, LI Qin-mei1,2,3. Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 750-754. |
[11] |
ZHAO Hong-kun1, 2, YU Tian3, XIAO Zhi-bo3, HAO Ya-bo4, LIU Ya-xuan1*. Homogeneity Test of Geochemical Certified Reference Materials by X-Ray Fluorescence Spectrometry With Pressed-Powder Pellets[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 755-762. |
[12] |
CHEN Hai-jie1, 2, MA Na1, 2, BO Wei1, 2, ZHANG Ling-huo1, 2, BAI Jin-feng1,2, SUN Bin-bin1, 2, ZHANG Qin1, 2, YU Zhao-shui1, 2*. Research on the Valence State Analysis Method of Selenium in Soil and Stream Sediment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 871-874. |
[13] |
ZHANG Jing1, GAO Xuan1, 2, JIN Liang1, WANG Hong-hui1, ZHOU Xi-ping1. Comparisons and Applications of Functional Equations for the Calculation of the Protein-Ligand Binding Constant Based on Fluorescence Spectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3494-3498. |
[14] |
CHEN Hai-jie1, 2, MA Na1, 2, BAI Jin-feng1, 2, CHEN Da-lei3, GU Xue1, 2, YU Zhao-shui1, 2, SUN Bin-bin1, 2, ZHANG Qin1, 2*. Study on Determination of Se in Geochemical Samples by External Supply H2-Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2896-2900. |
[15] |
XIONG Yang1,2, XU Jun1,2, QIU Su-yan1,2, WEI Yi-hua1,2, ZHANG Jin-yan1,2*. Study on Interaction Between Phenylethanolamine A and CdTe Nanomaterials by Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1402-1406. |
|
|
|
|