光谱学与光谱分析 |
|
|
|
|
|
Rapid and Noninvasive Detection of Skin Cholesterol with Diffuse Reflectance Spectroscopy Technology |
HOU Hua-yi1, 2, DONG Mei-li1, 3*, WANG Yi-kun1, 3, ZHU Ling1, 3, MA Zu-chang1, LIU Yong1, 3 |
1. Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China 2. University of Science and Technology of China, Hefei 230026, China 3. Wanjiang Center for Development of Emerging Industrial Technology, Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument, Tongling 244000, China |
|
|
Abstract Due to the urgent need for noninvasive detection of skin cholesterol, a portable, intelligent and real-time skin diffuse reflectance spectroscopy measurement system was designed based on a micro-spectrometer. Digitonin-horseradish peroxidase copolymer solution was prepared. According to the properties digitonin binds to the hydroxy of cholesterol molecular specifically and the horseradish peroxidase reacts with TMB color solution (the main component is 3,3’,5,5’-tetramethylbenzidine ) a color change was produced, by which the skin cholesterol was identified and instructed with high sensitivity and high specificity, and the concentration of skin cholesterol was quantified by measuring the degree of color change. In order to validate the feasibility of this method, pig skin which is similar to human skin was taken as the experimental subject, and cholesterol samples of gradient concentration were achieved through the extraction. After that the spectroscopy measurement system was adopted to detect the cholesterol concentration. The experiment result showed that, relative diffuse reflectance can distinguish the cholesterol samples with different concentrations, and the diffuse reflectance intensity factor can quantity the concentrations of cholesterol at characteristic wavelengths (442, 450 and 463 nm) and characteristic wavelength band of 442~500 nm. Linear fitting curves were obtained with the determination coefficient R2 were 0.960, 0.959, 0.958 and 0.958, respectively. The study has shown that, using diffuse reflectance spectroscopy technology can realize noninvasive rapid detection of skin cholesterol, and applying it to the risk assessment of atherosclerotic diseases would contribute to the prevention and control of such diseases significantly.
|
Received: 2015-08-04
Accepted: 2015-12-16
|
|
Corresponding Authors:
DONG Mei-li
E-mail: dongmeili@aiofm.ac.cn
|
|
[1] YE Ping, CHEN Hong, WANG Lü-ya(叶 平, 陈 红, 王绿娅). Dyslipidemia Aiagnosis and Treatment(血脂异常诊断和治疗). Beijing: People’s Military Medical Press(北京: 人民军医出版社), 2013. 34. [2] Sabine J R. Cholesterol. New York: Marcel Dekkar, 1977. [3] Khalilov E M, Fortinskaia E S, Nikitina N A, et al. Klinicheskaia Laboratornaia Diagnostika, 1994, 5: 12. [4] Bouissou H, Degraeve J, Legendre C, et al. Biomedicine & Pharmacotherapy, 1982, 36: 159. [5] Bouissou H, Degraeve J, Solera M L, et al. Archives Des Maladies Du Coeur Et Des Vaisseaux, 1982, 75: 621. [6] Bouissou H, Graeve J D, Thiers J C, et al. Biomedicine Express, 1979, 31: 236. [7] Weerheim A, Ponec M. Arch. Dermatol. Res., 2001, 293: 191. [8] Hiroshi Shiigi Hiroaki Matsumoto, Itaru Ota, Tsutomu Nagaoka. J. Flow Injection Anal., 2008, 25(1): 81. [9] John Mancini G B, Sammy Chan, Jiri Frohlich, et al. The American Journal of Cardiology, 2002, 89(1): 1313. [10] ZHANG Jun, CHEN Xing-dan, PIAO Ren-guan, et al(张 军, 陈星旦, 朴仁官, 等). Optics and Precision Engineering(光学精密工程), 2008, 16(6): 987. [11] JIANG Li-ying, YAO Fei-fei, CHEN Qing-hua, et al(姜利英, 姚斐斐, 陈青华, 等). Sensor World(传感器世界), 2010,(1): 6. [12] Torkhovskaya T I, Fortinskaya E S, Khalilov E M, et al. Biulleten’ Eksperimental’noǐ Biologii i Meditsiny, 1992, 113(5): 481. [13] FANG Yong-hua, KONG Chao, LAN Tian-ge, et al(方勇华, 孔 超, 兰天鸽, 等). Optics and Precision Engineering(光学精密工程), 2006, 14(6): 1089. [14] XIE Jun, PAN Tao, CHEN Jie-mei, et al(谢 军, 潘 涛, 陈洁梅, 等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(3): 342. [15] HUANG Fu-rong, PAN Tao, ZHANG Gan-lin, et al(黄富荣, 潘 涛, 张甘霖, 等). Optics and Precision Engineering(光学精密工程), 2010, 18(3): 588. |
[1] |
LIU Wen-bo, LIU Jin, HAN Tong-shuai*, GE Qing, LIU Rong. Simulation of the Effect of Dermal Thickness on Non-Invasive Blood Glucose Measurement by Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2699-2704. |
[2] |
GUO Yuan1, 2, HUANG Yi-xiang3, 4, HUANG Chang-ping3, 4, SUN Xue-jian3, 5, LUAN Qing-xian1*, ZHANG Li-fu3, 5*. Analysis of Blood Oxygen Content in Gingival Tissue of Patients With
Periodontitis Based on Visible and Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2563-2567. |
[3] |
YAN Xue-jun1, ZHOU Yang2, HU Dan-jing1, YU Dan-yan1, YU Si-yi1, YAN Jun1*. Application of UV-VIS Diffuse Reflectance Spectrum, Raman and
Photoluminescence Spectrum Technology in Nondestructive
Testing of Yellow Pearl[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1703-1710. |
[4] |
YAN Jun1, FANG Shi-bin1, YAN Xue-jun1, SHENG Jia-wei2, XU Jiang1, XU Chong3, ZHANG Jian2*. Study on the Common Effect of Heat Treatment, Dyeing or Irradiation Treatment on UV-Vis Diffuse Reflectance Spectra of Pearls[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3697-3702. |
[5] |
FANG Shi-bin1, JIANG Yang-ming1, YAN Jun1, 2, YAN Xue-jun1, ZHOU Yang3, ZHANG Jian2*. The Types of UV-Vis Diffuse Reflectance Spectra of Common Gray Pearls and Their Coloring Mechanism[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3703-3708. |
[6] |
WANG Jing1, 2*, CHEN Zhen3, GAO Quan-zhou1. Diffuse Reflectance Spectroscopy Study of Mottled Clay in the Coastal
Area of Fujian and Guangdong Provinces and the Interpretation of Its
Origin and Sedimentary Environment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2494-2498. |
[7] |
ZHU Meng-yuan1, 2, LÜ Bin1, 2*, GUO Ying2. Comparison of Haematite and Goethite Contents in Aeolian Deposits in Different Climate Zones Based on Diffuse Reflectance Spectroscopy and Chromaticity Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1684-1690. |
[8] |
XU Zhao-jin, LI Dong-liang, SHEN Li*. Study on Diffuse Reflection and Absorption Spectra of Organic and Inorganic Chinese Painting Pigments[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3915-3921. |
[9] |
XIE Xue-wei2, ZHONG Hao-chen2, CHEN Zhen-cheng2, HE Min2, ZHU Jian-ming1*. Detection of Advanced Glycosylation End Products by Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1055-1059. |
[10] |
WANG Peng1,2, SUN Di2, MU Mei-rui3, LIU Hai-xue3, ZHANG Ke-qiang2, MENG Xiang-hui1, YANG Ren-jie1*, ZHAO Run2*. Rapid Detection of Total Nitrogen Through the Manure Movement of in Large-Scale Dairy Farm by Near-Infrared Diffuse Reflectance Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3287-3291. |
[11] |
WANG Lin, MA Xue-jie, MENG Dan-rui, LIU Rong*, XU Ke-xin. Simulation and Experiment Study on Three-Dimensional Coordinate Outlier Detetion Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(09): 2774-2779. |
[12] |
WANG Zhi-mao, LIU Rong*, XU Ke-xin. Existence of Pressure-Insensitive Radial Position in Diffuse Reflection Contact Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2477-2482. |
[13] |
SUN Zhe1, HAN Tong-shuai1, 2, JIANG Jing-ying1*, LI Chen-xi1, 2, XU Ke-xin1, 2. Study on Surface Reflectance Light Elimination of Biological Tissue with Cross-Polarization[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(11): 3520-3524. |
[14] |
LIU Yong1,2, ZHANG Yuan-zhi1, HOU Hua-yi1, ZHU Ling1,2, WANG An1, WANG Yi-kun1,2*. Tissue Intrinsic Fluorescence Spectrum Recovering Based on Diffusion Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(12): 3836-3841. |
[15] |
LIU Yan1,2, YANG Xue1,2, ZHAO Jing3, LI Gang1,2, LIN Ling1,2* . Study on Internal Information of the Two-Layered Tissue by Optimizing the Detection Position[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(10): 3434-3441. |
|
|
|
|