| 光谱学与光谱分析 |
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| Discrimination of Lactarius and Russula Mushrooms with FTIR and Two-Dimensional Correlation Infrared Spectroscopy |
| MA Dian-xu, LIU Gang*, OU Quan-hong, YU Hai-chao, LI Hui-mei, LIU Yan |
| School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500, China |
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Abstract Tri-step infrared spectroscopy method of Fourier transform infrared spectroscopy, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy was firstly used to discriminate six species of mushrooms belonging to the genus Lactarius and Russula. The absorption bands of the original spectrum were very similar, which were composed by protein and polysaccharides, but tiny differences were observed at the position, shape and absorption intensities of peaks. Second derivative infrared spectroscopy technology was applied to study 6 species of the samples, there were obvious differences in the range of 1 800～1 400 and 1 200～800 cm-1. Two-dimensional correlation infrared spectroscopy can improve the resolution of spectra. Therefore two-dimensional correlation infrared spectroscopy was used to study 6 kinds of mushrooms. The results showed that there are three auto-peaks in the Lactarius, four in the Russula and significant differences in the number, intensity of auto-peaks and cross peaks were observed in the range of 1 690~1 420 cm-1. In addition, the peaks quantity, position, intensity of auto-peaks and cross peaks were different in the range of 1 110~920 cm-1. It demonstrates that tri-step infrared spectroscopy technology of Fourier transform infrared spectroscopy, second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy is a rapid and effective method for discriminating Lactarius and Russula.
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Received: 2015-04-10
Accepted: 2015-08-20
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Corresponding Authors:
LIU Gang
E-mail: gliu66@163.com
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[1] LIU Xiao-jiao, XU A-sheng, DENG Li-jun(刘小娇, 徐阿生, 邓丽君). Edible Fungi of China(中国食用菌), 2010, 29(4): 8.
[2] MAO Xiao-lan(卯晓岚). The Macrofungi in China(中国大型真菌). Zhengzhou: Henan Science and Technology Press(郑州：河南科学技术出版社), 2000.
[3] Ozen T, Darcan C, Aktop O, et al. Combinatorial Chemistry ＆ High Throughput Screening, 2011, 14(2): 72.
[4] Choong Y K, Sun S Q, Zhou Q, et a1. Journal of Molecular Structure, 2014, 1069: 229.
[5] Popescu C M, Popescu M C, Vasile C. Microchemical Journal, 2010, 95(2): 377.
[6] Lei Y, Zhou Q, Zhang Y L, et al. Journal of Molecular Structure, 2010, 974: 88.
[7] Tian Z H, Wu K, Liu W T, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015, 140(5): 356.
[8] Aneta B, Monika M O, Miroslaw K, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2011, 78(4): 1221.
[9] SUN Su-qin, ZHOU Qun, QIN Zhu(孙素琴, 周 群, 秦 竹). Atlas of Two-dimensional Correlation Infrared Spectroscopy for Traditional Chinese Medicine Identification(中药二维相关红外光谱鉴定图集). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2003.
[10] Li J R, Sun S Q, Wang X X, et al. Journal of Molecular Structure, 2014, 1069: 229.
[11] Choong Y K, Sun S Q, Zhou Q, et al. Vibrational Spectroscopy, 2011, 57: 87.
[12] DU Juan, PENG Xi-yuan, MA Fang, et al(杜 娟, 彭惜媛, 马 芳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2014, 34(9): 2439.
[13] Zhou Y Q, Yu H, Zhang Y L, et al. Journal of Molecular Structure, 2010, 974(3): 127.
[14] Li M Y, Cheng S C, Li D, et a1. Chinese Chemical Letters, 2015, 26: 221. |
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