| 光谱学与光谱分析 |
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| Intermolecular Interactions between Cytisine and Bovine Serum Albumin: A Synchronous Fluorescence Spectroscopic Analysis and Molecular Docking Research |
| WU Yu-hang1, HAN Zhong-bao1, MA Jia-ze1, HE Yan1, LIU Li-yan1, XIN Shi-gang2, YU Zhan1* |
1. School of Chemistry and Chemical and Engineering, Shenyang Normal University, Shenyang 110034, China
2. Experimental Center, Shenyang Normal University，Shenyang 110034, China |
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Abstract Cytisine (Cy) is one of the alkaloids that exist naturally in the plant genera Laburnum of the family Fabaceae. With strong bioactivities, Cy is commercialized for smoking cessation for years. In this work, the study of intermolecular interactions between Cy and bovine serum albumin (BSA) was performed by applying fluorescence spectroscopic methods under simulated physiological conditions. The mechanism of fluorescence quenching of BSA by Cy was also studied. Parameters such as bathing temperature, time and solution pH were investigated to optimize the fluorescence quenching. The binding type, binding ratio and binding constant between BSA and Cy were calculated by using the Stem-Volmer equation. Experimental results indicated that Cy can quench the fluorescent emission of BSA statically by forming a 1∶1 type non-covalent complex and the binding constant is 5.6×103 L·mol-1. Synchronous fluorescence spectral research shows Cy may affect the fluorescence emission of Trp residues of BSA. Furthermore, molecular docking is utilized to model the complex and probe the plausible quenching mechanism. It can be noted that the hydrogen bindings and hydrophobic interactions between Cy and BSA change the micro-environment of Trp213, which leads to the fluorescence quenching of BSA.
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Received: 2014-11-12
Accepted: 2015-03-12
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Corresponding Authors:
YU Zhan
E-mail: yuzhan@synu.edu.cn
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[1] HU Tao-ying, CHEN Lin, LIU Ying(胡涛英, 陈 琳, 刘 颖). Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2014, 34(5): 1343.
[2] Rouden J, Lasne M-C, Blanchet J, et al. Chemical Reviews, 2013, 114(1): 712.
[3] Tzankova V, Danchev N. Biotechnology & Biotechnological Equipment, 2007, 21(2): 151.
[4] Lin Z, Huang C F, Liu X S, et al. Basic & Clinical Pharmacology & Toxicology, 2011, 108(5): 304.
[5] Bujacz A. Acta Crystallogr. D, 2012, 68(10): 1278.
[6] Hanwell M, Curtis D, Lonie D, et al. Journal of Cheminformatics, 2012, 4(1): 17.
[7] Morris G M, Huey R, Lindstrom W, et al. Journal of Computational Chemistry, 2009, 30(16): 2785.
[8] Laskowski R A, Swindells M B. Journal of Chemical Information and Modeling, 2011, 51(10): 2778.
[9] Xu H, Yao N, Xu H, et al. International Journal of Molecular Sciences, 2013, 14(7): 14185.
[10] BI Shu-yun, LIU Chun-ming(毕淑云, 刘春明). Chinese Journal of Analytical Chemistry(分析化学), 2009, 37(z1): 933.
[11] Zhang X, Li L, Xu Z, et al. PLoS ONE, 2013, 8(3): e59106.
[12] Jayabharathi J, Jayamoorthy K, Thanikachalam V, et al. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2013, 108: 146.
[13] HE Li-fang, LIN Dan-li, LI Yao-qun(何立芳, 林丹丽, 李耀群). Progress in Chemistry(化学进展), 2004, 16(6): 879.
[14] Congdon R W, Muth G W, Splittgerber A G. Analytical Biochemistry, 1993, 213: 407. |
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