| 光谱学与光谱分析 |
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| Examination of Correlation between Histidine and Cadmium Absorption by Eleagnus angustifolia L., Vitisvinifera L. and Nerium oleander L. Using HPLC-MS and ICP-MS |
| Sukran Akkus Ozen, Mehmet Yaman* |
| Firat University, Faculty of Science, Department of Chemistry, Elazig, Turkey |
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Abstract In this study, HPLC-MS and ICP-MS methods wereused for the determination of histidine and cadmiumin Eleagnusangustifolia L., Vitisvinifera L. and Nerium oleander L. leaves taken from industrial area including Gaziantep and Bursa cities. To histidine determination by HPLC-MS, flow rate of mobile phase, fragmentor potential, injection volume and column temperature were optimized as 0.2 mL·min-1, 70 V, 15 μL and 20 ℃, respectively. For extraction of histidine from plants, distilled water was used by applying on 90 ℃ and 30 min. The concentrations (as mg·kg-1) of histidine were found to be in range of 8～22 for Eleagnusangustifolia L., 10～33 for Vitisvinifera L. and 6～11 for Nerium oleander L. The concentrations of cadmium were found to be in ranges of 6～21 μg·kg-1 for Vitisvinifera L. 15～110 μg·kg-1 for Eleagnusangustifolia L. and 63～218 μg·kg-1 for Nerium oleander L.
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Received: 2015-08-20
Accepted: 2015-10-09
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Corresponding Authors:
Mehmet Yaman
E-mail: ijpacmy@gmail.com; myaman@firat.edu.tr
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