光谱学与光谱分析 |
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Development of Fluorescence Resonance Energy Transfer Sensor for Determination of Adenosine Monophosphate in Biological Drug |
DONG Ling-yu, DU Hong-ming, WANG Peng, WANG Li-yun, LI Yi-ke, ZHAI Hong, FENG Ting, WANG Xiang-feng, ZHU Qiao-you, XIE Meng-xia* |
Analytical & Testing Center of Beijing Normal University, Beijing 100875, China |
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Abstract The biological drug of the calf-blood dialysate has various pharmacological effects. It can promote the oxygen and glucose uptake for the hypoxia cells, and has beneficial effects on the malfunction of the blood circulation and trophic disturbances in the brain, and the impairment of peripheral blood circulation. Furthermore, it is favorable to wound healing and can regulate the central nervous system. Adenosine monophosphate (AMP) is a main active ingredient of the biological drug. In this report, a fluorescence resonance energy transfer (FRET) sensor has been developed with β-CD-capped ZnS QDs as energy donor and 3-hydroxyflavone (3-HF) as energy acceptor. The results showed that AMP can lead to the fluorescence quenching of the FRET sensor at 526 nm, and the Stern-Volmer curve between the fluorescence quenching and the concentrations of AMP present a satisfactory linearity with the correlation coefficient of 0.996. The developed sensor has successfully applied for determination of the AMP in the biological drug.
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Received: 2015-02-09
Accepted: 2015-05-10
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Corresponding Authors:
XIE Meng-xia
E-mail: xiemx@bnu.edu.cn
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