| 光谱学与光谱分析 |
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| Rapid Determination of the Multi-Marker Ingredients in Heterosmilacis Japonicae Rhizoma and Sophorae Flavescentis Radix with Near-Infrared Diffused Reflection Spectroscopy |
| ZHAO Feng-chun, WAN Kai-yang, LIU Xiao-qian, ZHANG Qi-wei, GAO Hui-min*, WANG Zhi-min* |
| Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Beijing 100700, China |
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Abstract A rapid NIRS method for determination of macrozamin in Heterosmilacis japonicae rhizoma (HJR), and the total content of oxymatrine and matrine (OMT+MT) as well as the total content of oxysophocarpine and sophocarpine (OSC+SC) in sophorae flavescens radix (SFR) was developed to explore the application feasibility of NIRS for the quality assurance system of Chinese patent drugs. The contents of macrozamin in HJR samples, and OMT+MT and OSC+SC in SFR samples were determined by HPLC as reference values. The NIR spectra of the samples were measured in a diffused reflection mode. The different characteristic wavebands and pretreatment methods were optimized. The quantitative calibration models between the NIR spectra and the content reference values of marker components in HJR and SFR samples, were established with partial least square method, and further optimized through the cross validation and external validation. The contents of macrozamin in 88 batches of HJR samples were over the range of 0.36~12.88 mg·g-1. The total contents of OMT+MT and OSC+SC in 75 batches of SFR samples were over the range of 8.87~66.31 and 2.30~15.11 mg·g-1, respectively. The performance of the final models for macrozamin, OMT+MT and OSC+SC was evaluated well according to correlation coefficients (r), root mean square error of cross-validation (RMSECV) and root mean square error of prediction (RMSEP). The R2 values of the cross-validation for macrozamin, OMT+MT and OSC+SC were 0.902 5, 0.949 1 and 0.913 7, and those of RMSECV were 0.961, 2.45 and 0.724 mg·g-1 respectively. The R2 values of external validation for the three models were 0.981 7, 0.982 6 and 0.960 9, and those of RMSEP were 0.693, 2.27 and 0.658 mg·g-1, respectively. This is the first report on rapid determination of macrozamin in Heterosmilacis japonicae rhizoma, and oxymatrine, matrine, oxysophocarpine and sophocarpine in sophorae flavescens radix by NIRS method. The presented method can fulfill the requirement of rapid acquirement of chemical information of raw medicinal materials prior the manufacturing of compound Kushen injection.
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Received: 2014-05-08
Accepted: 2014-07-22
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Corresponding Authors:
GAO Hui-min, WANG Zhi-min
E-mail: huimin_gao@126.com;zhmw123@263.net
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