光谱学与光谱分析 |
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Photoinduced Electron Ttransfer between [Ru(bpy)3]2+ and Copper(Ⅱ) Ion in Composite Films Mediated by DNA |
JIANG Zhen-shen, CHEN Min-jian, LI Hong* |
School of Chemistry and Environment, South China Normal University, Guangzhou 510006, China |
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Abstract An orange-red [Ru(bpy)3]2+-DNA-Cu2+ composite film (bpy = 2,2′-bipyridine) was fabricated on an indium-tin (ITO) surface based on electrostatic interactions among [Ru(bpy)3]2+, DNA and Cu2+ by using self-standing cast methods. The photoinduced electron transfer (PET) properties of the resultant composite film mediated by DNA were studied by means of steady-state and time-resolved fluorescence spectroscopy, UV-visible absorption spectroscopy, fluorescence microscopic imaging and scan electron microscopy. The [Ru(bpy)3]2+-DNA-Cu2+ composite film with molar ratio of 10∶20∶1 shows an obvious absorption band (450 nm) and an intense emission peak (λem=595 nm), whose emission exhibits a single-exponential decay with τ=188.6 ns and is quenched by Cu2+ via DNA-mediated PET mechanism, indicating that the quenching constant is 6.94×103 L·mol-1 and quenching rate constant is 3.80×1010 L·mol-1·s-1. The increasing molar ratio of Cu2+ in composite films (10-fold) leads to an 11 nm blue-shift of the emission peak, which is dramatically weakened by Cu2+ via a static quenching mechanism. In addition, compared with the emission quenching of DNA-[Ru(bpy)2(tatp)]2+ (tatp=1,4,8,9-tetra-aza-triphenylene) tuned by Cu2+, which is present either in solutions or in composite films, Cu2+ only quenches the emission of [Ru(bpy)3]2+ bound to DNA via an electrostatic interaction mode in composite films.
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Received: 2013-01-07
Accepted: 2013-04-05
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Corresponding Authors:
LI Hong
E-mail: lihong@scnu.edu.cn
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