| 光谱学与光谱分析 |
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| Research on Detecting Concentration of Serum Protein Based on Resonance Rayleigh Scattering |
| WANG Gao, FENG Qiao-ling, XUE Zhong-jin, LI Yang-jun, ZHOU Han-chang |
| 1. Key Laboratory of Instrumentation Science and Dynamic Measurement, North University of China, Taiyuan 030051, China2. National Key Laboratory for Electronic Measurement Technology, North University of China, Taiyuan 030051, China |
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Abstract The resonance Rayleigh scattering spectral detection system was designed based on the 2,9,16,23-tetracarboxylate-phthalocyanine zinc and protein system. In the system, excitation light source is 405 nm wide band gap semiconductor lasers, and monochromator is 475 nm narrow-band band-pass filter, and the detector is low-noise and high-gain photoelectric amplifier based on blue-ray enhanced photodiode. Experiment shows that, the solution’s strong absorption wavelength is near 420 nm. Under the action of incentive light, resonance Rayleigh scattering is generated at the resonant wavelength, and the scattering intensity is proportional to the protein content. The system uses 2,9,16,23-tetracarboxylate as the spectrum probe to determine the concentration of serum proteins by resonance Rayleigh scattering method. Its linear detection range is 10 ~ 50 mg·mL-1, and its detection limit is 0.001 mg·mL-1. The newly developed device for detecting concentration of the serum protein has the advantages of small size, low cost, low power consumption, and being easy to use.
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Received: 2012-08-01
Accepted: 2012-10-30
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Corresponding Authors:
WANG Gao
E-mail: gxx@nuc.edu.cn
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