光谱学与光谱分析 |
|
|
|
|
|
Application of the Vanillin Sulfuric Acid Colorimetry-Ultraviolet Spectrometry on Quality Evaluation of Panax notoginseng |
DING Yong-li1,2, WANG Yuan-zhong1, ZHANG Ji1, ZHANG Qing-zhi2*, ZHANG Jin-yu1,2*, JIN Hang1,2 |
1. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650223, China 2. College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China |
|
|
Abstract In this study, Panax notoginseng samples were extracted by chloroform, ethanol and water, or by those extracted solution with 5% vanillin sulfuric acid to establish two kinds of UV fingerprint of P. notoginseng which were compared by applying the common and variation peak ratio dual index sequence analysis method and SIMCA software qualitative analysis. The results indicated that the optimization extraction time of P. notoginseng samples was 20 min with chloroform, ethanol and water extraction, but the fingerprint differed significantly after add vanillin sulfuric acid. The common peak ratios of UV fingerprint of P. notoginseng were scattered. The minimum was 25%(Y5—Y8), while the maximum was 84.38%(Y11—Y13,Y20—Y21). The maximum variation peak ratio was 177.78%(Y8—Y5), meanwhile, the variation peak ratios of several samples were more than 100%. However, the common peak ratios of UV fingerprint of P. notoginseng with vanillin sulfuric acid were concentrated (distributed in the range of 50%~70%): the minimum was 42. 86%(Y1—Y19), whereas the maximum was 79.55% (Y22—Y23); the range of the variation peak ratios was also smaller with the ranges of 20%~50% in general. The result of the dual index sequence analysis was agreement with the fingerprint implied. The similarity of the UV fingerprint of the extracts of P. notoginseng after adding vanillin sulfuric acid was greater than before. Both the ages and origin was related with the difference of UV fingerprint. The similarity of the two samples with same age was more significant than those with different ages. The similarity and difference between samples was no correlation with the distance of geographic space, the near origin samples maybe have a significant similarity or difference. This method appears as good alternative for evaluate quality of the P. notoginseng and can distinguish at least two samples quantitatively, duo to it reaches the limitation of the multiple methods which only could be used to indistinctly distinguish herbs.
|
Received: 2012-06-11
Accepted: 2012-09-10
|
|
Corresponding Authors:
ZHANG Qing-zhi, ZHANG Jin-yu
E-mail: ynkzqz@126.com; jyzhang2008@126.com
|
|
[1] China Pharmacopoeia Commission(国家药典委员会). Chinese Pharmacopoeia of the People’s Republic of China (Part One) (中华人民共和国药典,Ⅰ部). Beijing:China Medical Science Press (北京:中国医药科技出版社),2010. 11. [2] Wang C Z,Mcentee E,Wicks S,et al. Journal of Natural Medicines,2006,60(2):97. [3] Lv Y,Xu Y Y,Xu H Y,et al. Asian Journal of Pharmacodynamics and Pharmacokinetics,2008,8(4):253. [4] He N W,Zhao Y,Guo L,et al. Journal of Medicinal Food,2012,15(4):350. [5] Jia Y,Li Z Y,Zhang H G,et al. Journal of Ethnopharmacology,2010,132(1):297. [6] CHEN Jia-mo(陈嘉谟). Materia Medica Compaion(本草蒙筌). Beijing: People’s Medical Publishing House(北京:人民卫生出版社),1988. 1. [7] Xie P S,Chen S B,Liang Y Z,et al. Journal of Chromatography A,2006,1112(1-2):171. [8] Sárbu C,Nascu-Briciu R D,Kot-Wasik A,et al. Food Chemistry,2012,130 (4):994. [9] Casale M,Oliveri P,Armanino C,et al. Analytica Chimica Acta,2010,668 (2):143. [10] Basílio I J L D,Moura R K P,Bhattacharyya J,et al. Chemistry & Biodiversity,2012,9(6):1114. [11] Chen P,Luthria D,Harrington P B,et al. Journal of AOAC International,2011,94(5):1411. [12] Hiai S,Oura H,Odaka Y,et al. Planta Medica,1975,28(4):363. [13] Hiai S,Oura H,Odaka Y,et al. Planta Medica,1976,29(2):116. [14] LIU Yuan,MENG Qing-yan,PENG Lian-xin,et al(刘 圆,梦庆艳,彭镰心,等). Chinese Journal of Pharmaceeutical Analysis(药物分析杂志),2007,27(8):1182.
|
[1] |
YAO Kun-shan1, SUN Jun1*, CHEN Chen2, XU Min1, CHENG Jie-hong1, ZHOU Xin1. Non-Destructive Identification for Panax Notoginseng Powder of Different Parts Based on Hyperspectral Imaging Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2027-2031. |
[2] |
ZHANG Xue-fei1, DUAN Ning1, 2*, JIANG Lin-hua1, 2*, CHENG Wen2, YU Zhao-sheng3, LI Wei-dong2, ZHU Guang-bin4, XU Yan-li2. Study on Stability and Sensitivity of Deep Ultraviolet Spectrophotometry Detection System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3802-3810. |
[3] |
ZHANG Fu-jie, SHI Lei, LI Li-xia*, ZHAO Hao-ran, ZHU Yin-long. Study on Nondestructive Identification of Panax Notoginseng Powder Quality Grade Based on Hyperspectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(07): 2255-2261. |
[4] |
CHEN Feng-xia1, YANG Tian-wei2, LI Jie-qing1, LIU Hong-gao3, FAN Mao-pan1*, WANG Yuan-zhong4*. Identification of Boletus Species Based on Discriminant Analysis of Partial Least Squares and Random Forest Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 549-554. |
[5] |
LU Li-min, SHI Bin, TANG Tian-yu, ZHAO Xian-hao, WEI Xiao-nan, TANG Yan-lin*. Spectral Analysis of Epinephrine Molecule Based on Density Functional Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 248-252. |
[6] |
LIN Yan1, SU Jun-hong1*, TANG Yan-lin2, YANG Dan3. Ultraviolet Spectrum and Excitation Properties Calculations of Vitamin C Based on Density Functional Theory[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 304-309. |
[7] |
XU Hui-hua, SHI Dong-po*, WU Hao, YIN Xian-qing, ZHENG Yan-cheng, CHEN Wu, LI Geng. Influence of AEO-9 on Ultraviolet Absorbance Spectrum of TDBAC Reduced by β-CD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3931-3935. |
[8] |
YANG Lu-ze, LIU Miao*. Construction of a 3D-QSAR Model With Dual Spectral Effects and Its Application in Molecular Modification of Environmentally Friendly PBBs[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 430-434. |
[9] |
TONG Ang-xin, TANG Xiao-jun*, ZHANG Feng, WANG Bin. Species Identification of NaCl, NaOH and β-Phenylethylamine Based on Ultraviolet Spectrophotometry and Supervised Pattern Recognition Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 448-453. |
[10] |
CHEN Ying1,XU Yang-mei1, DI Yuan-jian1,CUI Xing-ning1,ZHANG Jie1,ZHOU Xin-de1,XIAO Chun-yan2, LI Shao-hua3. COD Concentration Prediction Model Based on Multi-Spectral Data Fusion and GANs Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 188-193. |
[11] |
YANG Hui-qin1, 2, ZHANG Bo1, 2, MA Ling1, 2, SHANG Yi1, 2, GAO Dong-li1, 2*. Extraction and Spectroscopic Analysis of Chlorogenic Acid in Diploid Potato[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3860-3864. |
[12] |
LI Xin1, SU Cheng-zhi1,2*, YU Dan-yang1, SHENG Yu-bo1, CHANG Chuan1, SHI Lei1, JIANG Ji-guang1. Study on the Influence of Wavelength and Low Temperature on COD Detection by Ultraviolet Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2403-2408. |
[13] |
HE Zhi-heng1, XU Rong2, LIN Jun-feng2, YAN Ning1, CHEN Chun-xia3, CHEN Run-quan3, CHAI Xin-sheng1, 3*. Tri-Wavelength UV Spectroscopy Method by Figuring out the Isobestic Points Shift for the Determination of Fluorescent Whitening Agents in Paper Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1758-1762. |
[14] |
QIU Jia-chu1,4, RUAN Ping2,4*, YONG Jun-guang3, FENG Bo-hua2, 4, HUANG Dai-zheng5, SHEN Hong-tao6. UV-Visible Absorption Spectra and FTIR of Hemoglobin of Healthy People and It Spectroscopic Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1425-1430. |
[15] |
XIONG Yuan-hui1, 2,LUO Zhong-jie1,CHEN Zhen-wei2, YU Guang-bao1, 2,DUAN Wei-min2, LIU Lin-mei2,LI Fa-quan2,WU Kui-jun2*. Study on Ultraviolet Imaging Remote Sensing Monitoring Technology for SO2 Gas Emission[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1289-1296. |
|
|
|
|