光谱学与光谱分析 |
|
|
|
|
|
Progress in Combination of Gel Electrophoresis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Trace Elements Determination in Proteins |
WANG Ying2, GUO Yan-li1, 2*, YUAN Hong-lin1*, WEI Yong-feng2, YAN Hong-tao2, CHEN Hui-hui2 |
1. State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University,Xi’an 710069, China 2. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, China |
|
|
Abstract Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become a very efficient and sensitive trace, ultratrace, and surface analytical technique for the in situ study of the concentration and distribution of the elements in life sciences with high spatial resolution. It is being used more and more frequently in biological, medical materials and protein research, which will lead to a better understanding of physiology and pathology process in cells and tissues. The present review mainly introduces the strategies of combination of gel electrophoresis (GE) with LA-ICP-MS for the quantification of trace elements in proteins, including the proteins separation, elements detection and calibration methods. The paper emphasizes the basic conditions of the proteins separation, focusing on the stability of proteins during GE and the treatment methods of staining and drying of the gel to enable successful detection of the elements by LA-ICP-MS. In addition, the application of GE-LA-ICP-MS in phosphoproteins, selenoproteins and metal-binding proteins is introduced in detail. The prospects and challenge for this technique are discussed as well for further study.
|
Received: 2011-07-25
Accepted: 2011-11-16
|
|
Corresponding Authors:
GUO Yan-li, YUAN Hong-lin
E-mail: guoyl-123@hotmail.com;hlyuan@263.net
|
|
[1] Hasnain S S. J. Synchrotron Radiat., 2004, 11: 7. [2] Szpunar. J. Anal. Bioanal. Chem., 2004, 378: 54. [3] Yuan H L, Gao S, Dai M N, et al. Chemical Geology, 2008, 247: 100. [4] Hare D, Tolmachev S, James A, et al. Anal. Chem., 2010, 82: 3176. [5] Dressler V L, Pozebon D, Mesko M F, et al. Talanta, 2010, 82: 1770. [6] Sussulini A, Kratzin H, Jahn O, et al. Anal. Chem., 2010, 82: 5859. [7] Stadlbauer C, Reiter C, Patzak B, et al. Anal. Bioanal. Chem., 2007, 388: 593. [8] Becker J S, Breuer U, Hsieh H F, et al. Anal. Chem., 2010, 82: 9528. [9] ZHENG Ling-na, WANG Meng, WANG Hua-jian, et al(郑令娜, 王 萌, 王华建, 等). Progress in Chemistry(化学进展) , 2010, 22(11):2199. [10] Hu S, Zhang S, Hu Z, et al. Anal. Chem., 2007, 79: 923. [11] Becker J S, Becker J S, Zoriy M V, et al. Eur. J. Mass Spectrom., 2007, 13: 1. [12] Garfn D E. TrAC, Trends Anal. Chem., 2003, 22: 263. [13] Becker J S, Zoriy M, Becker J S, et al. Phys. Stat. Sol. (C), 2007, 4: 1775. [14] Becker J S, Zoriy M, Becker J S, et al. J. Anal. At. Spectrom., 2007, 22: 736. [15] Krüger R, Kübler D, Pallisse R, et al. Anal. Chem., 2006, 78: 1987. [16] Becker J S, Zoriy M, Michael P, et al. International Journal of Mass Spectrometry, 2007, 261: 68. [17] Wind M, Feldmann I, Jakubowski N, et al. Electrophoresis, 2003, 24: 1276. [18] Ballihaut G, Pecheyran C, Mounicou S, et al. Trends Anal. Chem., 2007, 26: 183. [19] Ballihaut G, Tastet L, Pécheyran C, et al. J. Anal. At. Spectrom., 2005, 20: 493. [20] Chassaigne H, Chéry C C, Bordin G, et al. J. Anal. At. Spectrom., 2004, 19: 85. [21] Pedrero Z, Madrid Y, Cámara C, et al. Anal. At. Spectrom., 2009, 24: 775. [22] Binet M R, Ma R, McLeod C W, et al. Anal. Biochem., 2003, 318: 30. [23] Becker J S, Lobinski R, Becker J S. Metallomics, 2009, 1: 312. [24] Jiménez M S, Rodriguez L, Gomez M T, et al. Talanta, 2010, 81: 241. [25] Raab A, Pioselli B, Munro C, et al. Electrophoresis, 2009, 30: 303. [26] Deitrich C L , Braukmann S, Raab A, et al. Anal. Bioanal. Chem., 2010, 397: 3515. [27] Evans R D, Villeneuve J Y. J. Anal. At. Spectrom., 2000, 15: 157. [28] Allardyce C S, Dyson P J, Abou-Shakra F R, et al. Chem. Comm., 2001, 24: 2708. [29] Feldmann I, Koehler C U, Roos P H, et al. J. Anal. At. Spectrom., 2006, 21: 1006. [30] Marshall P, Heudi O, Bains S, et al. Analyst, 2002, 127: 459. [31] Venkatachalam A, Koehler C U, Feldmann I, et al. J. Anal. At. Spectrom., 2007, 22: 1023. [32] Jakubowski N, Waentig L, Hayen H, et al. J. Anal. At. Spectrom., 2008, 23: 1497. [33] Chéry C C, Günther D, Cornelis R, et al. Electrophoresis, 2003, 24: 3305. [34] Becker J S, Zoriy M, Krause-Buchholz U, et al. J. Anal. At. Spectrom., 2004, 19: 1236. [35] Becker J S, Zoriy M, Becker J S, et al. Anal. Chem., 2005, 77: 5851. [36] Krause-Buchholz U, Becker J S, Zoriy M, et al. Int. J. Mass Spectrom., 2006, 248: 56. [37] Boulyga S F, Heumann K G. Anal. Bioanal. Chem., 2005, 383: 442. [38] Fernández B, Claverie F, Pécheyran C, et al. Anal. Chem., 2008, 80: 6981. [39] Yang C K, Chi P H, Lin Y C, et al. Talanta, 2010: 1222. [40] Konz I, Fernández B, Fernández M L, et al. Anal. Chem., 2011, 83(13): 5353. [41] Hera I L, Palomo M, Madrid Y. Anal. Bioanal. Chem., 2011, 400: 1717. [42] Fan T W M, Pruszkowski E, Shuttleworth S. J. Anal. At. Spectrom., 2002, 17: 1621. [43] Ballihaut G, Claverie F, Pécheyran C, et al. Anal. Chem., 2007, 79: 6874. [44] Bandura D R, Ornatsky O I, Liao L. J. Anal. At. Spectrom., 2004, 19: 96. [45] Becker J S, Zoriy M, Becker J S, et al. J. Anal. At. Spectrom., 2004, 19: 149. [46] Becker J S, Zoriy M, Pickhardt C, et al. Int. J. Mass Spectrom., 2005, 242: 135. [47] Becker J S, Zoriy M, Przybylski M, et al. J. Anal. At. Spectrom., 2007, 22: 63. [48] Polatajko A, Azzolini M, Feldmann I, et al. J. Anal. At. Spectrom., 2007, 22: 878. [49] Ma R, McLeod C W, Tomlinson K, et al. Electrophoresis, 2004, 25: 2469. [50] Chery C C, Moens L, Cornelis R, et al. Pure Appl. Chem., 2006, 78: 91. [51] Becker J S, Mounicou S, Zoriy M V, et al. Talanta, 2008, 76: 1183. |
[1] |
HE Yan1, TAO Ran1, YANG Ming-xing1, 2*. The Spectral and Technology Studies of Faience Beads Unearthed in Hubei Province During Warring States Period[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3700-3709. |
[2] |
WANG Wei-en. Analysis of Trace Elements in Ophiocordyceps Sinensis From
Different Habitats[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3247-3251. |
[3] |
WANG Yan1, HUANG Yi1, 2*, YANG Fan1, 2*, WU Zhong-wei2, 3, GUAN Yao4, XUE Fei1. The Origin and Geochemical Characteristics of the Hydrothermal Sediments From the 49.2°E—50.5°E Hydrothermal Fields of the Southwest Indian Ocean Ultra-Slow Spreading Ridge[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2868-2875. |
[4] |
CHEN Chao-yang1, 2, LIU Cui-hong1, 2, LI Zhi-bin3, Andy Hsitien Shen1, 2*. Alexandrite Effect Origin of Gem Grade Diaspore[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2557-2562. |
[5] |
CHEN Di, SONG Chen, SONG Shan-shan, ZHANG Zhi-jie*, ZHANG Hai-yan. The Dating of 9 Batches of Authentic Os Draconis and the Correlation
Between the Age Range and the Ingredients[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1900-1904. |
[6] |
HE Yan1, SU Yue1, YANG Ming-xing1, 2*. Study on Spectroscopy and Locality Characteristics of the Nephrites in Yutian, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3851-3857. |
[7] |
CHEN Chao-yang1,HUANG Wei-zhi1,SHAO Tian1,LI Zhi-bin2,Andy Hsitien Shen1*. Characteristics of Visible Spectrum of Apatite With Alexandrite Effect[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1483-1486. |
[8] |
LIU Yan-de, GAO Xue, JIANG Xiao-gang, GAO Hai-gen, LIN Xiao-dong, ZHANG Yu, ZHENG Yi-lei. Detection of Anthracnose in Camellia Oleifera Based on Laser-Induced Breakdown Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2815-2820. |
[9] |
LU Xiao-ke1, LI Wei-dong1, LI Xin-wei2. Spectroscopic Analysis of Relics Unearthed from Xipo Site[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1186-1194. |
[10] |
LIANG Piao-piao1, ZHOU Shan-shan1, XING Yun-xin1, LIU Ying1, 2*. Quantification of Trace Elements in Hair Samples from 156 Women Living in the Low-Selenium Region of Inner Mongolia by ICP-AES and AFS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2217-2222. |
[11] |
HAO Xiao-jian*, TANG Hui-juan, HU Xiao-tao. Detection Sensitivity Improvement Study of LIBS by Combining Au-Nanoparticles and Magnetic Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1599-1603. |
[12] |
LIU Hong-wei, NIE Xi-du*. Analysis of Trace Elements in Wild Artemisia Selengensis Using Inductively Coupled Plasma Tandem Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(12): 3923-3928. |
[13] |
CAI Shi-shi1,ZHANG En1, 2*. Trace Elements and U-Pb Ages of Zircons from Myanmar Jadeite-Jade by LA-ICP-MS: Constraints for Its Genesis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1896-1903. |
[14] |
JIANG Bo1, 3, HUANG Jian-hua2*, LIU Wei2. Multi-Element Analysis of Wild Chinese Honeylocust Fruit by Inductively Coupled Plasma Tandem Mass Spectrometry (ICP-MS/MS)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3859-3864. |
[15] |
PENG Chuan-yi, ZHU Xiao-hui, XI Jun-jun, HOU Ru-yan, CAI Hui-mei*. Macro- and Micro-Elements in Tea (Camellia sinensis) Leaves from Anhui Province in China with ICP-MS Technique: Levels and Bioconcentration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1980-1986. |
|
|
|
|