| 光谱学与光谱分析 |
|
|
|
|
|
| A Comparison of Different Treatment Conditions on the Conformation Changes of Bovine Lactoferrin |
| WEN Peng-cheng1, 2，YU Dan-dan3，WANG Xin-xin1，ZHANG Lu-da2，REN Fa-zheng2，LEI Xin-gen4，GUO Hui-yuan2* |
1. College of Food Science & Engineering，Gansu Agricultural University，Lanzhou 730070，China
2. Key Laboratory of Functional Dairy，Ministry of Education，China Agricultural University，Beijing 100083，China
3. Beijing Higher Institution Engineering Research Center of Animal Product，Beijing 100083，China
4. Cornell University，Ithaca，New York，USA |
|
|
|
|
Abstract In this study，the tertiary，secondary structures and disulfide bond changes of bovine lactoferrin (bLF) under 6 differents physico-chemical treatments were investigated by fluorescence，circular dichroism(CD) and UV-Vis absorption.A red shift from 333 to 354 nm in the fluorescence emission maximum (λmax) was observed in the bLF treated by 6 mol·L-1 GdnHCl，8 mol·L-1 Urea and 50 mmol·L-1 DTT simultaneously，meanwhile a large number of exposed hydrophobic groups were detected.However，there was no marked shift in λmax of bLF treated by heating (100 ℃，5 min)，Ultrosonic(450 W，5 s，6 pulses) or β-ME(1%)， of which fluorescence intensity decreased significantly compared with the untreated bLF. The results indicated that the mechanism of changes in tertiary structure of the former three methods were different from the latter three. The detection by CD showed that the α- helix structure vanished completely in the bLF treated by GdnHCl. However，there was no remarkable change in the secondary structure of the bLF treated by the other five methods.In addition，UV-Vis absorption suggested that disulfide bond was seriously destructed in the bLF treated by DTT and Ultrosonict，but GdnHCl，β-ME and heating induced a little damage merely. This study is instructive and meaningful to the further research on relationship between structure and activity of bLF.
|
|
Received: 2011-03-30
Accepted: 2011-06-20
|
|
|
|
Corresponding Authors:
WEN Peng-cheng
E-mail: guohuiyuan99@gmail.com
|
|
[1] Metzboutigue M H，Jolles J，Mazurier J，et al. European Journal of Biochemistry, 1984，145(3)：659.
[2] Naot D，Grey A M D，Reid L R，et al. Clinical Medicine & Research, 2005，3(2)：93.
[3] Ye X Y，Wang H X，Liu F, et al. International Journal of Biochemistry & Cell Biology, 2000，32(2)：235.
[4] Abe H, Saito H, Miyakawa H, et al. Journal of Dairy Science, 1991, 74(1): 65.
[5] Sargent P J，Farnaud S，Evans R W. Current Medicinal Chemistry, 2005，12(23)：2683.
[6] Kelly S M，Price N C. Bba-Protein Structm, 1997，1338(2)：161.
[7] Wang L C. Journal of Food Science, 1984，49(2)：551.
[8] Perez-Iratxeta C，Andrade-Navarro M A. BMC Structural Biology, 2008，8(25)：1.
[9] Anderson M E. Methods in Enzymology, 1985，113：548.
[10] Burstein E A，Vedenkin N S，Ivkova M N. Photochemistry and Photobiology, 1973，18(4)：263.
[11] Sreedhara A，Flengsrud R，Prakash V，et al. International Dairy Journal, 2010，20(7)：487.
[12] Joel D M，Joshua S K，Henry J P. Biochemistry, 1973，12：1290.
[13] Woody R W. Tetrahedron-Asymmetry, 1993，4(3)：529.
[14] Woody R W. Biochemical Spectroscopy, 1995，246：34. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [12] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [13] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [14] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [15] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
|
|
|
|
