| 光谱学与光谱分析 |
|
|
|
|
|
| Rapid Detection of Escherichia coli O157∶H7 Using Surface Plasmon Resonance (SPR) Biosensor |
| SI Cheng-yan, YE Zun-zhong*, WANG Yi-xian, GAI Ling, WANG Jian-ping, YING Yi-bin |
| College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China |
|
|
|
|
Abstract A biosensor based on surface plasmon resonance (SPR) was developed for rapid detection of Escherichia coli O157∶H7. BIACORE 3000 SPR instrument and a dextran-modified sensor chip (CM5) were used. After activation with EDC/NHS, anti-E. coli O157∶H7 antibody was immobilized on the gold surface of the SPR sensor, and then following ethanolamine was injected, and the chip was ready for E. coli O157∶H7. Regeneration was achieved using NaOH in order to detect several samples. The limit of detection was found to be 3×105 CFU·mL-1 for E. coli O157∶H7, and the change of RU was linearly correlated with the concentration of E. coli O157∶H7 cells (R2=0.99). The detection time ranged from 5 to 7 min, and the result of regeneration was effective which allowed the chip to be reused for more than 50 samples. This method is convenient, and stable, and shows potentials for applications in food areas.
|
|
Received: 2011-01-10
Accepted: 2011-05-06
|
|
|
|
Corresponding Authors:
YE Zun-zhong
E-mail: zzye@zju.edu.cn
|
|
[1] SONG Hong-xin, LI Hong(宋宏新, 李 宏). Food Science(食品科学), 2007, 28(11): 607.
[2] Lazcka O, Campo Del F J, Munoz F X. Biosensors and Bioelectronics, 2007, 22: 1205.
[3] Shankaran D R, Gobi K V, Miura N. Sensors and Actuators B, 2007, 121: 158.
[4] Bergwerff A A, Knapen F V. Journal of AOAC International, 2006, 89(3): 826.
[5] ZHANG Wen-hao, ZHENG Xian-zhe, CHU Guo-chao(张文浩, 郑先哲, 初国超). Journal of Northeast Agricultural University(东北农业大学学报), 2010, 46(6): 117.
[6] Waswaa J, Irudayaraj J, Cebroy C. LWT-Food Science and Technology, 2007, 40: 187.
[7] Dudak F C, Boyaci I H. Food Research International, 2007, 40: 803.
[8] Taylor A D, Ladd J, YU Qiu-ming, et al. Biosensors and Bioelectronics, 2006, 21: 752.
[9] Waswa J W, Debroy C, Irudayaraj J. Journal Food Process Engineering, 2006, 29: 373.
[10] Leonard P, Hearty S, Quinn J, et al. Biosensors and Bioelectronics, 2004, 19: 1331.
[11] Eum N S, Yeom S H, Kwon D H, et al. Sensors and Actuators B, 2010, 143: 784.
[12] Jung J, Na K, Lee J, et al. Analytica Chimica Acta, 2009, 651: 91.
[13] TANG Qian-qian, WANG Jian-ping, YE Zun-zhong, et al(唐倩倩, 王剑平, 叶尊忠, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(10): 2618.
[14] Lee H J, Kim B C, Kim K W, et al. Biosensors and Bioelectronics, 2009, 24: 3550.
[15] Sun Y, Song D Q, BAI Yu-ping, et al. Analytica Chimica Acta, 2008, 624: 294.
[16] Guo Z H, Fan X, Liu L K, et al. Journal of Colloid and Interface Science, 2010, 348: 29. |
| [1] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
| [2] |
GUO Ge1, 3, 4, ZHANG Meng-ling3, 4, GONG Zhi-jie3, 4, ZHANG Shi-zhuang3, 4, WANG Xiao-yu2, 5, 6*, ZHOU Zhong-hua1*, YANG Yu2, 5, 6, XIE Guang-hui3, 4. Construction of Biomass Ash Content Model Based on Near-Infrared
Spectroscopy and Complex Sample Set Partitioning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3143-3149. |
| [3] |
CHENG Fang-beibei1, 2, GAN Ting-ting1, 3*, ZHAO Nan-jing1, 4*, YIN Gao-fang1, WANG Ying1, 3, FAN Meng-xi4. Rapid Detection of Heavy Metal Lead in Water Based on Enrichment by Chlorella Pyrenoidosa Combined With X-Ray Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2500-2506. |
| [4] |
LI Bin, SU Cheng-tao, YIN Hai, LIU Yan-de*. Hyperspectral Imaging Technology Combined With Machine Learning for Detection of Moldy Rice[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2391-2396. |
| [5] |
ZHANG Jing, GUO Zhen, WANG Si-hua, YUE Ming-hui, ZHANG Shan-shan, PENG Hui-hui, YIN Xiang, DU Juan*, MA Cheng-ye*. Comparison of Methods for Water Content in Rice by Portable Near-Infrared and Visible Light Spectrometers[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2059-2066. |
| [6] |
FAN Chun-hui1, 2, YUAN Wen-jing1, XIN Yi-bei1, GUO Chong1, LAN Meng-xin1, JIANG Zhi-yan1. Spectral Characteristics of Dissolved Organic Matter (DOM) in Reclaimed Water Used for Agricultural Irrigation in Water-Deficient Area for the Dual Carbon Targets[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1465-1470. |
| [7] |
HUANG Xiao-wei1, ZHANG Ning1, LI Zhi-hua1, SHI Ji-yong1, SUN Yue1, ZHANG Xin-ai1, ZOU Xiao-bo1, 2*. Detection of Carbendazim Residue in Apple Using Surface-Enhanced Raman Scattering Labeling Immunoassay[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1478-1484. |
| [8] |
SHI Zhi-feng1, 2, LIU Jia2, XIAO Juan2, ZHENG Zhi-wen1*. Investigation of Novel Method for Detecting Vanillin Based on X-Ray Diffraction Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1563-1568. |
| [9] |
WANG Yi-tao1, WU Cheng-zhao1, HU Dong1, SUN Tong1, 2*. Research Progress of Plasticizer Detection Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1298-1305. |
| [10] |
DONG Xin-xin, YANG Fang-wei, YU Hang, YAO Wei-rong, XIE Yun-fei*. Study on Rapid Nondestructive Detection of Pork Lean Freshness Based on Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 484-488. |
| [11] |
LIU Feng-xiang, HE Shuai, ZHANG Li-hao, HUANG Xia, SONG Yi-zhi*. Application of Raman Spectroscopy in Detection of Pathogenic Microorganisms[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3653-3658. |
| [12] |
CHEN Yu-nan1, 2, 3, YANG Rui-fang1, 3*, ZHAO Nan-jing1, 3*, ZHU Wei1, 2, 3, CHEN Xiao-wei1, 2, 3, ZHANG Rui-qi1, 2, 3. Research on Measuring Oil Film Thickness Based on Laser-Induced Water Raman Suppression Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3954-3962. |
| [13] |
YAN Kang-ting1, 2, HAN Yi-fang1, 2, WANG Lin-lin1, 2, DING Fan3, LAN Yu-bin1, 2*, ZHANG Ya-li2, 3*. Research on the Fluorescence Spectra Characteristics of Abamectin Technical and Preparation Solution[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3476-3481. |
| [14] |
ZHANG Qian, DONG Xiang-hui, YAO Wei-rong, YU Hang, XIE Yun-fei*. Surface-Enhanced Raman Spectroscopy for Rapid Detection of Flunixin Meglumine Residues in Pork[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3155-3160. |
| [15] |
HU Yu-xia1, CHEN Jie1, SHAO Hui1, YAN Pu1, XU Heng1, SUN Long1, XIAO Xiao1, XIU Lei3, FENG Chun2GAN Ting-ting2, ZHAO Nan-jing2*. Research Progress of Spectroscopy Detection Technologies for Waterborne Pathogens[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2672-2678. |
|
|
|
|
