| 光谱学与光谱分析 |
|
|
|
|
|
| Immunonanogold Catalytic Spectrophotometric Determination of Trace IgG |
| DENG Jun-yao1,2,ZHANG Sheng-sen1,JIANG Zhi-liang1,3* |
1. School of Environment and Resource, Guangxi Normal University, Guilin 541004, China
2. Children-Women Health Hospital of Guilin City, Guilin 541001, China
3. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China |
|
|
|
|
Abstract Gold nanoparticles the size of about 10 nm were prepared by improved trisodium citrate reduction procedure, and were used to label goat anti-human IgG to obtain a sensitive spectral probe for IgG in the condition of pH 6.5. The immune reaction of nanogold-labeled IgG antibody (anti-IgG) with the antigen IgG took place to form the nanogold immune complex in pH 7.0 Na2HPO4-C6H8O7 buffer solution and in the presence of polyethylene (PEG). The optimal immunoreaction conditions were pH 7.0, 10.76 μg·mL-1 nanogold-labeled anti-IgG, 8.0% PEG 6000 and incubation time of 30 min under the ultrasonic irradiation. After centrifuging for 15 min at 16 000 rpm, the excess nanogold-labeled anti-IgG in the upper solutions was obtained, and was used to catalyze the colored particle reaction of HAuCl4 with NH2OH·HCl to produce gold particles with bigger size. The influence of pH value, HAuCl4 and NH2OH·HCl concentration, and reaction temperature and time on the immunonanogold catalytic reaction was considered spectrophotometrically. A pH 2.27 Na3C6H5O7-HCl buffer solution, 0.094 mmol·L HAuCl4, 1.92 mmol·L NH2OH·HCl, and reaction time of 6 min at 30 ℃ water bath were chosen for use. Results demonstrated that with increasing IgG, the concentration of gold labeled anti-IgG in the upper solution decreased, and the absorbance decreased linearly. Linear relationships between the decreased absorbance at 700 nm and the IgG concentration CIgG in the range of 0.10-10 ng·mL-1 were obtained. Its regress equation was ΔA760 nm＝0.014 4ｃIgG+0.004 2, the related coefficient was 0.992 6, and the detection limit reached 0.06 ng·mL-1 IgG. The influence of foreign substances on the determination of 3 ng·mL-1 IgG was examined, with the relative error ±10%. Results showed that the following coexistent substances had no impact on the assay: 6 000 ng·mL-1 HSA, 6 000 ng·mL-1 gluocose, 6 000 ng·mL-1 Zn(Ⅱ)，3 000 ng·mL-1 IgA, 3 000 ng·mL-1 Ca(Ⅱ), 3 000 ng·mL-1 L-arginine, 3 000 ng·mL-1 β-phenylalanine，2 400 ng·mL-1 Cu(Ⅱ)，2 400 ng·mL-1 EDTA, 2 400 ng·mL-1 L-cystinol etc. This showed that the assay has high selectivity. The sensitive, rapid and highly specific assay was applied to the quantification of IgG in human sera, with satisfactory results.
|
|
Received: 2007-08-28
Accepted: 2007-12-02
|
|
|
|
Corresponding Authors:
JIANG Zhi-liang
E-mail: zljiang@mailbox.gxnu.edu.cn
|
|
[1] Chu X, Fu X, Chen K, et al. Biosensors Bioelectronics, 2005, 20: 1812.
[2] Sun X L, Zhao X L, Tang J, et al. Int. J. Food Microbiology, 2005, 99(2): 185.
[3] Jiang Z L, Sun S J, Liang A H, et al. Clin. Chem., 2006, 52: 1389.
[4] Taton T A, Mirkin C A, Letsinger R L. Science，2000，289: 1757.
[5] Brown K R, Fox A P, Natan M J. J. Am. Chem. Soc.，1996，118: 1148.
[6] Lyon L A, Musick M D, Natan M J. Anal. Chem.，1998，70: 5177.
[7] Dansher G, Norgaard S O, J. Histochem. Cytomchem.，1983，31: 394.
[8] Park S J, Taton T A, Mirkin C A. Science，2002，295: 1503.
[9] Maya Z, Ronan B, Inna P, et al. Nano Lett., 2005，5: 21.
[10] Eliades P, Karagouni E, Stergiatou I, et al. J. Immunol. Methods, 1998，218: 123.
[11] Sumi M G, Mathai A, Sarada C, et al. J. Clin. Microbiol.，1999，37: 3925.
[12] Ma Z F, Sui S F. Angew. Chem. Int. Ed.，2002, 41: 2176.
[13] DENG Jun-yao, SUN Shuang-jiao, JIANG Zhi-liang, et al(邓俊耀, 孙双姣, 蒋治良, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006，26(8)：1487.
[14] FAN Ai-ping, LIU Cai-yun, LIAO Zhi-xin, et al(樊爱萍, 刘彩云, 廖志新, 等). Chin. J. Anal. Chem.(分析化学), 2006, 34: 35.
[15] MOU Lan, CHEN Xiao-kang, LI Long-di(牟 兰, 陈小康, 李隆弟). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(6): 880.
[16] Mao X, Jiang J H, Luo Y, et al. Talanta，2007, 73(3)：420.
[17] LI Hua, ZHANG Xin-shen, ZOU Yu-quan, et al(李 华, 张新申, 邹玉权, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(4): 785.
[18] YU Yun, SHI Wen-jian, YANG Ning-fei(俞 芸, 施文健, 杨凝非). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(3): 573. |
| [1] |
ZHANG Rong1, 2, DUAN Ning1, 3, JIANG Lin-hua1, 3*, XU Fu-yuan3, JIN Wei3, LI Jian-hui1. Study on Optical Path Optimization for Direct Determination of
Spectrophotometry of High Concentration Hexavalent Chromium
Solution by Ultraviolet Visible Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1829-1837. |
| [2] |
YAO Shan1, ZHANG Xuan-ling1, CAI Yu-xin1, HE Lian-qiong1, LI Jia-tong1, WANG Xiao-long1, LIU Ying1, 2*. Study on Distribution Characteristics of Different Nitrogen and
Phosphorus Fractions by Spectrophotometry in Baiyangdian
Lake and Source Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1306-1312. |
| [3] |
LIU Qin-rong1, DU Zi-wei1, LI Jia-zhen1, WANG Yi-shuo1, 3*, GU Xuan2, CUI Xiu-mei2. Analysis and Evaluation of Inorganic Elements in Salvia miltiorrhiza and Rhizosphere Soils From Different Areas[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(11): 3618-3624. |
| [4] |
LI Zhen-xia1, SHEN Huan-huan1, GAO Miao-miao1, QI Jiao1, LI Qing-yan2*. Analysis of Lycopene in Different Solvents by Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(04): 1114-1117. |
| [5] |
YANG Ze-ming1, 2, LI Cai1*, LU Gui-xin1, CAO Wen-xi1. A Study of Fast Detection of Nitrite in Seawater Based on Sequential Injection Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 589-595. |
| [6] |
LINGYUN Xia-fei1, 2, GAO Chao1, 2, FAN Jing-shuang1, 2, Lü Hai-xia1, 2*, YU Yan1, 2. Synthesis of Hyperbranched Polyamidoamine (PAMAM) Grafted Chitosan and Its Adsorption for Heavy Metal and Dyes Studied with Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3583-3587. |
| [7] |
CHEN Jing1, 2, 4, PENG Jiao-yu1, 2, BIAN Shao-ju1, 3, GAO Dan-dan1, 3, DONG Ya-ping1, 2*, LI Wu1, 3. Optimization of Determination of CO2-3 and HCO-3 in Boron-Containing Brine[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2194-2199. |
| [8] |
LI Yun1,2,3, ZHANG Ji1,2, LIU Fei4, XU Fu-rong3, WANG Yuan-zhong1,2*, ZHANG Jin-yu1,2,3*. Prediction of Total Polysaccharides Content in P. notoginseng Using FTIR Combined with SVR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1696-1701. |
| [9] |
WU Xin1，2, LI Guang-lin1*, WEN Zhi-yu3. Study and Determination the Concentration of CNO-Ion of the QPQ with the Sequential Injection Spectrophotometric Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1824-1828. |
| [10] |
CAO Xuan1, 2，ZHANG Shu-wei1, 2，CHU Dong-zhi1, 2，WU Ning1, 2，MA Hai-kuan1, 2，MA Ran1, 2. Development of Lab-on-Chip Spectrophotometric Silicate Sensor in situ Analysis of Seawater[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 895-900. |
| [11] |
HE Qiu-ju1，2, WANG Li-qin1*, ZHANG Ya-xu1. Study of Mechanism of Aluminum Sizing Precipitant on Xuan Paper Based on Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 418-423. |
| [12] |
ZHU Hong-qiu, GONG Juan, LI Yong-gang*, CHEN Jun-ming. A Spectrophotometric Detecting Method of Trace Cobalt under High Concentrated Zinc Solution[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3882-3888. |
| [13] |
GONG Hong-ling1, LIU Yan-hui2*, TANG Yan-lin2*, HU Lin2. Effects of Temperature on DNA Condensation Detected with Temperature-Changed Ultraviolet Spectrum Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1831-1837. |
| [14] |
LIU Qian3, CHEN Wen-juan1,2, JING Bo1,2, ZHANG Jian1,2, YANG Meng-ying3, CHEN Wu3, YIN Xian-qing1,3*. Spectrum Analysis of Trace Hydrogen Peroxide in Electrochemical Process[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1656-1660. |
| [15] |
XIE Ying-ke1,2,3, WEN Qun1,2*, WEN Zhi-yu1,2, MO Zhi-hong1,2, WEI Kang-lin1,2. On-Line Determination of Chemical Oxygen Demand and Total Phosphorus in Water by Using Fenton Reagents via a Micro-Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1642-1648. |
|
|
|
|
