光谱学与光谱分析 |
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Research on the Interaction of Cr(Ⅲ) Complex of Genistein with DNA |
YU Yan-ying1,LI Hua1,HU Xin1,CAO Shu-wen2* |
1. Department of Chemistry, Nanchang University, Nanchang 330031, China 2. Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China |
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Abstract The interaction of the Cr(Ⅲ) complex of genistein (GEN-Cr) with calf thymus DNA (ctDNA) in Tris (pH 7.2) buffer was investigated using UV spectra, DNA melting, fluorescence spectra and viscosity. From the absorption titration experiment, no obvious red shift was found, but the notable hypochromicities were observed. When cDNA/cGEN-Cr=3, the π→π* transitions of the complex at 272 nm showed a decrease in intensity of 29.1%, which indicated that there was remarkable intercalation between complex and DNA base pairs, involving a strong π-stacking interacting between them. The binding constant for the complex was K=1.9×105 mol·L-1. From the melting curves of ctDNA in the absence and presence of the complex, the melting temperature of ctDNA was found to increase by 5.5 ℃ from 74 to 79.5 ℃, owing to the increased stability of the helix in the presence of the complex that was intercalated into the double helix. The complex could emit weak luminescence in Tris buffer. The emission intensity of the complex at 340 nm increased steadily with the addition of ctDNA. The result suggested that the complex got into a hydrophobic environment inside the DNA and avoided the effect of solvent water molecules. The strong interaction of the complex and ctDNA also resulted in greatly enhanced intensity of the resonance light scattering spectra. The emission intensity of DNA-EB system at 600 nm decreased remarkably with increasing the complex concentration, which indicated that the complex could be intercalated into DNA and replace EB from the DNA-EB system. According to the classical Stern-Volmer equation, the quenching plots at 25 and 37 ℃ both appeared approximately linear. These results showed that there was one predominant quenching style in this process. Viscosity experiments were carried out by an Ubbelodhe viscometer at 20.0(±0.1)℃. The relative viscosity of ctDNA increased steadily with the increas in the complex. The result clearly showed that the complex could be intercalated between DNA base pairs, causing an extension of the helix, and thus increased the viscosity of DNA. The results above indicated that there is a relatively strong interaction between the GEN-Cr complex and ctDNA, and the complex could bind ctDNA mainly by intercalation. The research suggested that the GEN-Cr complex may be a promising candidate for anticancer, which deserves further research.
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Received: 2007-03-02
Accepted: 2007-06-09
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Corresponding Authors:
CAO Shu-wen
E-mail: cswyyy@nc.jx.cn
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[1] YANG Pin, SONG Yu-fei(杨 频, 宋宇飞). Progress in Chemistry(化学进展), 2000, 12(1):32. [2] JI Liang-nian(计亮年). World Science-Technolog R & D(世界科技研究与发展), 2004, 26(6):1. [3] Richard A Dixon, Daneel Ferreira. Phytochemistry, 2002, 60(3):205. [4] FAN Juan, SHEN Rui, TANG Ning. Journal of Rare Earths(稀土), 2004, 22(Suppl.):25. [5] WANG Ping-hong, ZHANG Qi, YUAN Wen-bing, et al(王平红, 张 岐, 袁文兵, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(7):1298. [6] WU Hong-xing, LI Feng-hua, LIN Hai, et al(吴红星, 李凤华, 林 海, 等). Chinese Journal of Inorganic Chemsitry(无机化学学报), 2005, 21(1):117. [7] ZHOU Qing-hua, YANG Pin(周庆华,杨 频). Acta Chim. Sinica(化学学报), 2006, 64(8):793. [8] KANG Jing-wan, WU Hai-xia, LU Xiao-quan, et al(康敬万, 吴海霞, 卢小泉, 等). Chem. J. Chinese Universities(高等学校化学学报), 2005, 26(6):997. [9] Xiao S, Lin W, Wang C, et al. Med. Chem. Lett., 2001, 11:437. [10] Satyanarayana S, Daborusak J C, Chaires J B. Biochemistry, 1993, 32:2573. [11] LIN Qiu-yue, HU Rui-ding, ZHENG Xiao-hua(林秋月, 胡瑞定, 郑孝华). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(8):988. [12] Li K A, Ma C Q, Liu Y, et al. Chin. Sci. Bull, 2000, 45:386. [13] Zhou Q H, Yang P. Inorganica Chimica Acta, 2006, 359:1200. [14] LI Zhi-liang, CHEN Jian-hua, ZHANG Kai-cheng, et al(李志良, 陈建华, 章开诚, 等). Science in China(Series B)(中国科学, B辑), 1991, 21:1193. [15] GUO Yu-hua, CHEN Xiao-lan, ZHANG Ting, et al(郭玉华, 陈晓岚, 张 婷, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(3):475. [16] Sigma D S, Mazuder A, Perrin D M. Chem. Rev., 1993, 93:2295. [17] Satyanarayana S, Daborusak J C, Chaires J B. Biochemistry, 1992, 31:9319. [18] Palanisamy U M, Mallayan P. Inorganica Chimica Acta, 2004, 357:901. |
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