| 光谱学与光谱分析 |
|
|
|
|
|
| Effect of UV on the Absorption Spectra of E.coli Cells |
| YANG Tian-you1,2,LI Zong-wei1,ZHANG Feng-qiu1,CHEN Lin-hai1,LI Zong-yi1,QIN Guang-yong1*,HUO Yu-ping1 |
1. Provincial Key Laboratory of Ion Beam Bioengineering,Zhengzhou University,Zhengzhou 450052,China
2. Life Science and Technology College,Henan Institute of Science and Technology,Xinxiang 453003，China |
|
|
|
|
Abstract The present article studied the effects of different solutions in sample cavity on the spectra of E.coli. It is shown that the solutions of 2.8 mL distilled water and 0.1 mL LB and 0.1 mL PBS is the best,because it not only supplies good living condition but also has less effect on the spectra of E.coli. And it was found that UV in spectrophotometer has no effect on the mensuration result. On the basis of these studies the absorption spectra of E.coli irradiated by different doses UV were investigated. It is shown that there is a declining-rising-declining phenomenon in the spectra of E.coli with the dose increasing. This reflects the wound healing process of E.coli. Spectroscopic technique is helpful for the study of cell’s wound healing.
|
|
Received: 2006-05-26
Accepted: 2006-08-28
|
|
|
|
Corresponding Authors:
QIN Guang-yong
E-mail: yangtianyou2004@163.com
|
|
| Cite this article: |
|
YANG Tian-you,LI Zong-wei,ZHANG Feng-qiu, et al. Effect of UV on the Absorption Spectra of E.coli Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1600-1602.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2007/V27/I08/1600 |
[1] WANG Jing-yan,ZHU Sheng-geng,XU Chang-fa(王镜岩,朱圣庚,徐长法). Biochemistry 3rd ed(生物化学,第3版). Beijing: Higher Education Press(北京: 高等教育出版社),2002,145. 507.
[2] GUO Yao-jun(郭尧君). Technique of Spectral Photometer and Its Application in Biochemistry(分光光度技术及其在生物化学中的应用). Beijing: Science Press(北京: 科学出版社),1987. 223.
[3] GE Xiang-hong,ZHAO Yuan-li,ZHANG Feng-qiu,et al(葛向红,赵元黎,张凤秋，等). Acta Laser Biology Sinica(激光生物学报),2004,3(5): 383.
[4] Eyten Osnat,Seia Ben-ami,Abraham Katzir. Applied Optics,2000,39(19):3357.
[5] WANG Le-xin,ZHAO Zhi-min,YAO Hong-bing,et al(王乐新,赵志敏,姚红兵,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002,22(6): 980.
[6] XIE Ji-yun,JIANG Zhi-liang,ZHONG Fu-xin,et al(谢济运,蒋治良,钟福新,等). Analysis and Testing Technology and Instruments(分析测试技术与仪器),2000,6(3): 178.
[7] Kiyoshi Yoshino(Janpan),JIANG Wei(Translator)(山田健一(日)，姜 伟，译). China Light & Lighting(中国照明电器),2005,(8): 24.
[8] XU Zhou-min,YU Li,LOU Fang-ding,et al(徐周敏,于 力,楼方定,等). Chin J. Radiol Med Prot(中华放射医学与防护杂志),2005,25(4): 398.
[9] SONG Dao-jun,WANG Ji,YAO Jian-ming,et al(宋道军,王 纪,姚建明,等). Acta Laser Biology Sinica(激光生物学报),1999,8(1): 19.
[10] GUO Ji-kui,WU Xiao-rong,JIANG Yan,et al(高继魁，吴晓蓉，蒋 彦，等). Journal of Siciuan univarsity(四川大学学报)，1998，35(3)： 477.
[11] HUANG Zhan-jing(黄占景). Hereditas(遗传)，1995，17： 81.
[12] Vonach R. Buschmarn J. Applied Spectroscopy,1998,52(6):820. |
| [1] |
ZHENG Hong-quan, DAI Jing-min*. Research Development of the Application of Photoacoustic Spectroscopy in Measurement of Trace Gas Concentration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 1-14. |
| [2] |
CHENG Jia-wei1, 2，LIU Xin-xing1, 2*，ZHANG Juan1, 2. Application of Infrared Spectroscopy in Exploration of Mineral Deposits: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 15-21. |
| [3] |
FAN Ping-ping，LI Xue-ying，QIU Hui-min，HOU Guang-li，LIU Yan*. Spectral Analysis of Organic Carbon in Sediments of the Yellow Sea and Bohai Sea by Different Spectrometers[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 52-55. |
| [4] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
| [5] |
WANG Fang-yuan1, 2, HAN Sen1, 2, YE Song1, 2, YIN Shan1, 2, LI Shu1, 2, WANG Xin-qiang1, 2*. A DFT Method to Study the Structure and Raman Spectra of Lignin
Monomer and Dimer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 76-81. |
| [6] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
| [7] |
XU Tian1, 2, LI Jing1, 2, LIU Zhen-hua1, 2*. Remote Sensing Inversion of Soil Manganese in Nanchuan District, Chongqing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 69-75. |
| [8] |
YANG Cheng-en1, 2, LI Meng3, LU Qiu-yu2, WANG Jin-ling4, LI Yu-ting2*, SU Ling1*. Fast Prediction of Flavone and Polysaccharide Contents in
Aronia Melanocarpa by FTIR and ELM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 62-68. |
| [9] |
LIU Zhen1*, LIU Li2*, FAN Shuo2, ZHAO An-ran2, LIU Si-lu2. Training Sample Selection for Spectral Reconstruction Based on Improved K-Means Clustering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 29-35. |
| [10] |
YANG Chao-pu1, 2, FANG Wen-qing3*, WU Qing-feng3, LI Chun1, LI Xiao-long1. Study on Changes of Blue Light Hazard and Circadian Effect of AMOLED With Age Based on Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 36-43. |
| [11] |
GAO Feng1, 2, XING Ya-ge3, 4, LUO Hua-ping1, 2, ZHANG Yuan-hua3, 4, GUO Ling3, 4*. Nondestructive Identification of Apricot Varieties Based on Visible/Near Infrared Spectroscopy and Chemometrics Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 44-51. |
| [12] |
ZHENG Pei-chao, YIN Yi-tong, WANG Jin-mei*, ZHOU Chun-yan, ZHANG Li, ZENG Jin-rui, LÜ Qiang. Study on the Method of Detecting Phosphate Ions in Water Based on
Ultraviolet Absorption Spectrum Combined With SPA-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 82-87. |
| [13] |
XU Qiu-yi1, 3, 4, ZHU Wen-yue3, 4, CHEN Jie2, 3, 4, LIU Qiang3, 4 *, ZHENG Jian-jie3, 4, YANG Tao2, 3, 4, YANG Teng-fei2, 3, 4. Calibration Method of Aerosol Absorption Coefficient Based on
Photoacoustic Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 88-94. |
| [14] |
LI Xin-ting, ZHANG Feng, FENG Jie*. Convolutional Neural Network Combined With Improved Spectral
Processing Method for Potato Disease Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 215-224. |
| [15] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
|
|
|
|
