| 光谱学与光谱分析 |
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| FTIR Spectra Analysis of the Reactive Activity of Lignin When Modified by Laccase |
| QIU Wei-hua, CHEN Hong-zhang* |
| State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China |
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Abstract In company with the development of nonaqueous enzymology, the enzymatic modification of lignin has gained increasing interests, especially in the synthesis of high molecular material. In the present article, the enzymatic modification of spruce alkali lignin in cetyltrimethylammonium bromide (CTAB) reversed micelles (100 mmol·L-11, pH 6.0, W/O=40), alcohol lignin in ethanol solution (50%), lignin sulphonate in sodium phosphate buffer (pH 5.8, 20 mmol·L-1) and steam-explosion wheat straw alkali lignin in alkaline solution (pH 10.0, 20 mmol·L-1 NaOH) by mycelia sterilia YY-5 laccase was studied. Laccase was isolated from Mycelia Sterilia YY-5 (CGMCC-1462) which was an entophytic fungus of Rhus Chinensis Mill. FTIR spectrum was used to assay the structure of lignins and gel permeation chromatography (GPC) was used to determine the molecular weight and molecular weight polydispersity of lignins. Bands of lignin in FTIR spectra of all lignins changed obviously after treated with YY-5 laccase, which indicated that some bond breakage or rearrangement occurred to lignin. The shift of non-conjugated C=O and conjugated carbonyl groups (α-carbonyl groups) stretching vibration, the decrease of phenol hydroxyl stretching vibration and the increase of C—O—C stretching vibration of ester bond proved that phenolic hydroxyl, carbonyl group and side chain substituent all might participate in the laccase modification reactions of lignin. Meanwhile, the results of GPC indicated that the molecular of lignins all have certain increase and molecular polydispersity decreased. From the point of the molecular mass polydispersity, the modification effect of YY-5 laccase on steam-exploded wheat straw alkali lignin and spruce alkali lignin was more significantly than other two lignins. The molecular mass polydispersity for steam-exploded wheat straw alkali lignin and spruce alkali lignin was 1.211 and 1.375 respectively, which might contribute to the alkali-stable enzyme for YY-5 laccase. Correspondingly, alkali solution was chosen as the optimum medium for YY-5 laccase to modify lignins.
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Received: 2007-05-10
Accepted: 2007-08-20
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Corresponding Authors:
CHEN Hong-zhang
E-mail: hzchen@home.ipe.ac.cn
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［1］ L Xiao-jing, YANG Jun, WANG Di-zhen, et al (吕晓静, 杨 军, 王迪珍, 等). Chemical Industry and Engineering Progress(化工进展), 2001, 20(5)：10.
［2］ Ciobanu C, Ungureanu M, Ignat L, et al. Industrial Crops and Products, 2004, 20：231.
［3］ Cetin Nihat S, zmen Milgül. International Journal of Adhesion & Adhesives, 2002, 22：481.
［4］ LI Feng-qi, ZHU Shu-quan(李凤起, 朱书全). Fine Petrochemicals Industry(精细石油化工), 2001, (2)：15.
［5］ Yaropolov A, Vartanov S, Varfolomeyev S D. Appl. Biochem. Biotechnol., 1994, 49：257.
［6］ Fernaud J R Hernández, Carnicero A, Perestelo F, et al. Enzyme and Microbial Technology, 2006, 38(1/2)：40.
［7］ Mai C, Schormann W, Hüttermann A. Enzyme and Microbial Technology, 2001, 28：460.
［8］ Johannes C, Majcherczyk A. Appl. Environ. Microbiol., 1999, 66：524.
［9］ Anderson Guerra, André Ferraz. Enzyme and Microbial Technology, 2001, 28：308.
［10］ Chen, Hongzhang, Liu Liying. Bioresource Technology, 2007, 98：666.
［11］ Xiao B, Sun X F, Sun Runcang. Polymer Degradation and Stability, 2001, 74：307.
［12］ QIU Wei-hua, CHEN Hong-zhang (邱卫华, 陈洪章). China Patent(中国发明专利) 2005, 200510086586. 9.
［13］ Liu Junhong, Yuan Weiping, Tao Lo. Electronic Journal of Biotechnology, 1999, 2(2)：82.
［14］ DI Xing-lian, LI Zhong-zheng, WANG Chuan-huai, et al(耿兴莲, 李忠正, 王传槐, 等). Chemistry and Industry of Forest Products(林产化学与工业), 1999, 19(1)：11.
［15］ WU Xin-sheng, XIE Yi-min, LIU Huan-bin, et al(吴新生, 谢益民, 刘焕彬, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(6)：1031.
［16］ Tejado A, Pena C, Labidi J, et al, et al. Bioresource Technology, 2007, 98(8)：1655.
［17］ CHEN Yun-ping, CHEN Rui-qiang, CHEN Xie-su, et al(陈云平, 陈瑞强, 程贤, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006, 26(10)：1880.
［18］ Xiao B, Sun X F, Sun Runcang. Polymer Degradation and Stability, 2001, 71：223.
［19］ SU Cheng-shou(苏承寿). Journal of Zhejiang Forestry College(浙江林学院学报), 1990, 7(1)：87.
［20］ QIU Wei-hua, CHEN Hong-zhang(邱卫华, 陈洪章). Journal of Cellulose Science and Technology(纤维素科学与技术), 2006, 14(1)：52. |
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