| 光谱学与光谱分析 |
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| Study on Binding Mechanism of Meso-tetra-(3,5-Dibromo-4-Hydroxyphenyl) Porphyrin with Protein by Fluorescence Method |
| YAN Mei,CHEN Xin,SUN Shu-ting,MA Hong-min,DU Bin,WEI Qin* |
| School of Chemistry and Chemical Engineering, University of Ji’nan, Ji’nan 250022, China |
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Abstract Studies on the binding mechanism between protein and small molecules could give us lots of useful information. For example, a detailed characterization of drug-protein binding properties was essential for understanding the function of delivery, hence, interest in the mechanism of the interaction between them has attracted much research using different methods. In the present paper, the interaction mechanism between meso-tetra-(3,5-dibromo-4-hydroxyphenyl) porphyrin [T(DBHP)P] and bovine serum albumin (BSA) was investigated using fluorescence method. Based on the mechanisms of fluorescence quenching of BSA caused by T(DBHP)P, the binding constants between T(DBHP)P and BSA were measured at different temperatures. The experiment showed that T(DBHP)P and BSA have strong interactions. The binding constants of the reaction at 27 and 48 ℃ were calculated by fluorescence method, respectively. The binding constants are K=1.30×106 L·mol-1 at 27 ℃, and K=6.32×105 L·mol-1 at 48 ℃. Because the binding constants decreased with increasing the temperature, the sort of quenching between T(DBHP)P and BSA was determined as static quenching. By the theory of Frster non-radiation energy transfer, the binding distance and the energy transfer efficiency at 27 ℃ between T(DBHP)P (accepter of energy) and BSA (donor of energy) were obtained to be 2.39 nm and 0.91, respectively. The binding distance was less than 7 nm, therefore, the interaction was similar to the non-radiation energy transfer, and the static quenching was further proved. According to the thermodynamic parameters, the main sorts of binding force between T(DBHP)P and BSA could be judged as electrostatic force when ΔG<0，ΔH<0 and ΔS>0. Using the synchronous fluorescence spectra, the effect of T(DBHP)P on the conformation of BSA was studied. The results indicated that the conformation of BSA was changed when T(DBHP)P was added, and the hydrophobic properties of the environment of residues in BSA decreased. It was proved that fluorescence quenching of BSA was induced by static quenching and non-radiation energy transfer.
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Received: 2007-08-26
Accepted: 2007-11-29
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Corresponding Authors:
WEI Qin
E-mail: sdjndxwq@263.net
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[1] YU Tian-zhi, TAO Zu-yi(俞天智, 陶祖贻). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(3): 453.
[2] Gharibi H, Javadian S, Hashemianzadeh M. Colloids and Surfaces A, 2004, 232: 77.
[3] Ohyoshi E, Hamada Y, Nakata K, et al. Inorganic Biochemistry, 1999, 75: 213.
[4] Guillaume Y C, Peyrin E, Berthelot A. Journal of Chromatography B, 1999, 728: 167.
[5] Jiang C Q, Gao M X, Meng X Z. Spectrochim Acta Part A, 2003, 59: 1605.
[6] Sun H Z, Szeto K Y. J. Inorganic Biochemistry, 2003, 94: 114.
[7] CAO Xi-min, DU Li-ming(曹玺珉, 杜黎明). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(5): 973.
[8] Wu D, Du B, Ma H M, et al. Spectroscopy Letters, 2006, 39: 399.
[9] JIANG Zhi-qiang, CHI Yan-hua, ZHUANG Jia, et al(蒋志强, 迟燕华, 庄 稼, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(5): 986.
[10] FEN Xi-zeng, BAI Chun-li, LIN Zhang, et al(冯喜增, 白春礼, 林 璋, 等). Chinese Journal of Analytical Chemisty(分析化学), 1998, 26(2): 154.
[11] DUAN Cai-hong, CHEN Xin, SUN Shu-ting, et al(段彩虹, 陈 欣, 孙舒婷, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(5): 969.
[12] Burstein E A, Vedenkina N S, Ivkova M N. Photochemistry and Photobiology, 1973, 18: 263.
[13] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智, 郑朱梓, 等). Methods of Fluorescence Analysis, 2nd Ed(荧光分析法, 第2版). Beijing: Science Press(北京: 科学出版社), 1990. 112.
[14] Ware W R. Journal of Physical Chemistry, 1962, 66(3): 455.
[15] YANG Man-man, YANG Pin, ZHANG Li-wei(杨曼曼, 杨 频, 张立伟). Chinese Science Bulletin(科学通报), 1994, 39(1): 31.
[16] Cristobal G, Dos R, Pierre D J M. Journal of Structural Biology, 1995, 115: 175.
[17] Ross D P, Subramanian S. Biochemistry, 1981, 20 (11): 3096.
[18] MA Chun-qi, LI Ke-an, ZHAO Feng-lin, et al(马春琪, 李克安, 赵凤林, 等). Acta Chimica Sinica(化学学报), 1999, 57(4): 389.
[19] Brustein E A, Vedenkina N S, Irkova M N. Photochem. Photobio. , 1973, 18: 26. |
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