光谱学与光谱分析 |
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Study on the Determination of Bovine Serum Albumin Using o-Hydroxyphenylfluorone Reagent with Fluorescence Spectrophotometry |
HUANG Ying-ping1,GU Yan2,FANG Yan-fen1,LIU Yu-liang1,ZHU Sheng-ji1,LOU Guang-fu1 |
1. China Three Gorges University Alan G. MacDiarmid Institute for Renewable Energy, Yichang 443002, China 2. College of Mechanical and Material Engineering, China Three Gorges University, Yichang 443002, China |
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Abstract The reactions were studied for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent with fluorescence spectrophotometry. In the B-R medium, o-HPF reacts with BSA to form the stable complex compound. The reaction system of o-HPF and BSA was binary system, and the method for the determination of BSA was of good selectivity and stability and simplicity. The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 μg·mL-1 The quenching constant and thermodynamics constant of the fluorescence effects between BSA and o-HPF were calculated. The results showed that the non-covalent binding forces were the binding force between o-HPF and BSA.
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Received: 2006-10-26
Accepted: 2007-01-29
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Corresponding Authors:
HUANG Ying-ping
E-mail: huangyp@ctgu.edu.cn
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