光谱学与光谱分析 |
|
|
|
|
|
Chemiluminescence Determination of 6-Mercaptopurine in the Luminol-Potassium Ferricyanide System |
WANG Tao1, XUE Bing-chun1, LIU Er-bao1,2* |
1. College of Chemistry and Material Science, Shanxi Normal University, Linfen 041000,China 2. Laboratory of Water Resources and Water Environment, Tianjin Normal University, Tianjin 300074, China |
|
|
Abstract A novel chemiluminescence (CL) method to determine 6-mercaptopurine (6-MP) is described, based on the catalytic effect of 6-MP on the CL reaction of luminol with K3Fe(C-N)6 in a flow system. Under optimal experimental conditions, the calibration graph is linear in the range of 1.02×10-7 to 1.02×10-9 mol·L-1 6-MP. The linear regression equation was Y=36.315c+140.72, where Y is the relative emission intensity and c is the concentration of 6-MP expressed in 10-8 mol·L-1 and the correlation coefficient is 0.998 2 with the detection limits of 6.33×10-10 mol·L-1(S/N=3). CL peak-height precision is 3.54% of RSD(n=12) for a level of 2.0×10-9 mol·L-1. This method satisfactorily provides a fast, simple, sensible and favorable technique for the determination of 6-MP in synthesized samples in implementation. Moreover, the possible mechanism of CL enhancement was briefly discussed. Obviously, the reaction was related to the formation of free radicals. Thus, the CL emission could preliminarily be attributed to the process of energy transfer due to O2. radical and ·OH radical resulting from the oxidation product of 6-MP by K3Fe(CN)6, which acts as an active intermediate that reacts with luminol, leading to the formation of excited luminol molecule, which gives out an emission when it returns to its ground state.
|
Received: 2007-11-02
Accepted: 2008-02-08
|
|
Corresponding Authors:
LIU Er-bao
E-mail: liueb123@163.com
|
|
[1] Rmstrong V W, Oellerich M. Clinical Biochemistry, 2001, 34: 9. [2] Cao Xuni, Lin Li, Zhou Yuyan, et al. Talanta, 2003, 60: 063. [3] Boulieu R, Dervieux T. J. Chromatogr. B, 1999, 730: 273. [4] Tsutsumi K, Otsuki Y, Kinoshita T. J. Chromatogr. B, 1982, 231: 393. [5] Tawa R, Takeshima S, Hirose S. Biochemical Medicine, 1984, 32: 303. [6] Gningue D, Aaron J J. Talanta, 1985, 32: 183. [7] Ding T L, Benet L Z. J. Chromatogr. B, 1979, 163: 281. [8] Kurzawa J. Anal. Chimi. Acta, 1984, 173: 343. [9] XUE Bing-chun, WANG Tao, LIU Er-bao(薛冰纯,王 滔,刘二保). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(5): 816. [10] Raman A, Oommen I K, Sharma D N. Applied Radiation and Isotopes, 2001, 54: 387. [11] WANG Shu-hao,DU Ling-yun,WEI Xin-ting,et al(王术皓, 杜凌云, 魏新庭, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005,25(5): 678. [12] Wiezchowski J, Slawinska D, Slawinska J. Z. Phys. Chem. Neue Folge, 1986, 148: 197. [13] Zhang Sichun, Ju Huangxian. Anal. Chimi. Acta, 2003, 475: 163. |
[1] |
CHEN Di, SONG Chen, SONG Shan-shan, ZHANG Zhi-jie*, ZHANG Hai-yan. The Dating of 9 Batches of Authentic Os Draconis and the Correlation
Between the Age Range and the Ingredients[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1900-1904. |
[2] |
HAO Jun1, WANG Yu2, LIU Cong2, WU Zan2, SHAO Peng2, ZU Wen-chuan2*. Application of Solution Cathode Glow Discharge-Atomic Emission Spectrometry for the Rapid Determination of Calcium in Milk[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3797-3801. |
[3] |
TANG Ju1, 2, DAI Zi-yun2*, LI Xin-yu2, SUN Zheng-hai1*. Investigation and Research on the Characteristics of Heavy Metal Pollution in Children’s Sandpits Based on XRF Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3879-3882. |
[4] |
WANG Guo-shui1, GUO Ao2, LIU Xiao-nan1, FENG Lei1, CHANG Peng-hao1, ZHANG Li-ming1, LIU Long1, YANG Xiao-tao1*. Simulation and Influencing Factors Analysis of Gas Detection System Based on TDLAS Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3262-3268. |
[5] |
ZHENG Pei-chao, ZHONG Chao, WANG Jin-mei*, LUO Yuan-jiang, LAI Chun-hong, WANG Xiao-fa, MAO Xue-feng. Evaluation of Flow Injection-Solution Cathode Glow Discharge With an Interference Filter Wheel for Spectral Discrimination[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 842-847. |
[6] |
YANG Hui-qin1, 2, ZHANG Bo1, 2, MA Ling1, 2, SHANG Yi1, 2, GAO Dong-li1, 2*. Extraction and Spectroscopic Analysis of Chlorogenic Acid in Diploid Potato[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3860-3864. |
[7] |
ZHOU Ying1, BAI Yong-hui1, SONG Xu-dong1*, YAO Min3, WANG Jiao-fei1, SU Wei-guang1, YU Guang-suo1,2*. Application of Chemiluminescence in Spectral Diagnosis: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3358-3364. |
[8] |
LI Ling1, 2, 3, NAI Xue-ying1, 2*, CHAI Xiao-li1, 2, 3, LIU Xin1, 2, GAO Dan-dan1, 2, DONG Ya-ping1, 2. Optimization of Determination Method of Lithium in Oil-Field Water Based on DOE[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2617-2621. |
[9] |
WU Sheng-yang1,2, HU Ren-zhi1,2*, XIE Pin-hua1,2, LI Zhi-yan1,2, LIU Xiao-yan3, LIN Chuan1,4, CHEN Hao1,2, WANG Feng-yang1,2, WANG Yi-hui1,5, JIN Hua-wei1,2. Real-Time Measurement of NOy (Total Reactive Nitrogen Oxide) by Cavity Ring Down Spectrometer (CRDS)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(06): 1661-1667. |
[10] |
LIU Yao, TAN Jian-guo*, GAO Zheng-wang. College of Aerospace Science and Engineering, National University of Defense Technology, Changsha 410073, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1018-1022. |
[11] |
ZHANG Jiu-ming1, 2, ZHOU Bao-ku1, WEI Dan3*, CHI Feng-qin1*, HAO Xiao-yu1, JIN Liang1, KUANG En-jun1. Spectroscopic Characteristics of HA Structure in Black Soil with Organic and Inorganic Fertilizer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(03): 845-850. |
[12] |
NIE Li-xing, CHANG Yan, DAI Zhong, MA Shuang-cheng*. A New Method for Determination of Polysorbate 80 in Shengmai Injection Based on Absorption Coefficient[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(01): 199-203. |
[13] |
YU Jie1, ZHU Shu-wen1, LU Quan-fang1, 2*, ZHANG Zhi-chao1, ZHANG Xiao-min1, WANG Xing1, YANG Wu1. High Sensitive Determination of Pb and Zn in Refined Copper Ores Samples Using Liquid Cathode Glow Discharge-Atomic Emission Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3550-3557. |
[14] |
WANG Zhong-yuan1, ZHANG Hong-kang2*, NI Zhi-xin1, LIU Jing-qin1, CHEN Xin1, LI Sheng-yong1, YANG Qi-jin2, ZHANG Can1. FI-KR-FAAS Twice Gas Isolation Elution Method for the Determination of Trace Lead in Marine Biological Samples[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3578-3582. |
[15] |
XIE Feng-ying1, LI Feng-feng1, ZHANG Shuang1, BI Wei-wei2, ZHANG Xiu-ling1*, ZHANG Xiao-nan1. Analysis of Acylation Modification of Black Rice Anthocyanins Using Fourier Transform Infrared Spectroscopy (FTIR)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2386-2389. |
|
|
|
|