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Molecular Structure of Two Glutamate Decarboxylases From Mung Bean [Vigna Radiate (L.)] Analyzed by Spectroscopy |
WANG Xian-qing1*, WEI Tong1, YANG Yong2*, SHI Yan-guo3 |
1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319,China
2. Beijing Shunxin Holdings, Beijing 101300, China
3. College of Food Science, Harbin University of Commrce, Harbin 150076,China |
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Abstract Glutamate decarboxylase (GAD) catalyzes an α-decarboxylation reaction of glutamate to produce γ-aminobutyric acid (GABA). Interestingly, two kinds of mung bean [Vigna radiate (L.)] GADs were firstly extracted and purified in this research. Two GADs in mung bean were both dimers with a molecular mass of 155 kDa (GAD1) and 75 kDa (GAD2). The enzymatic properties of GAD1 and GAD2 were detected in this research. Infrared spectroscopy analysis revealed that more higher-ordered structure contents, α-helix and β-sheet structures, was found in GAD2, which determined the higher stability of GAD2. It was analyzed by Raman that the molecular structures of GAD1 and GAD2 are generally “exposed”. Fluorescence analysis revealed that GAD1 had a more flexible and exposed molecular structure, while the conformation of GAD2 was more compact and conservative. It was found that pH and temperature-induced structural change decreased the enzyme activity. Ca2+ was involved in binding the calmodulin-binding domain of GADs and induced a “buried” and compact structure. The unfolding of GAD induced by SDS, impaired the enzyme activity. KI, MgSO4, AgNO3, and SDS significantly inhibited GAD1 and GAD2 activities. Tween 80, Ca2+ and Cu2+ could significantly activate GAD1 and GAD2, and Fe2+ only increased GAD2 activity.
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Received: 2019-10-23
Accepted: 2020-09-25
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Corresponding Authors:
WANG Xian-qing, YANG Yong
E-mail: yangyong7904@163.com; xqwang1977@126.com
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[1] Wu T N, Chen C K, Lee C S, et al. Scientific Reports, 2019, 9: 10255.
[2] Zhang H, Yao H Y, Chen F, et al. Food Chemistry, 2007, 101(4): 1670.
[3] Spink D C, Porter T G, Wu S, et al. Biochemical Journal, 1985, 231(3): 695.
[4] Jin W J, Kim M J, Kim K S. Journal of Food Science, 2013, 78(9): 1376.
[5] Ueno H. Journal of Molecular Catalysis B: Enzymatic, 2000, 10(1-3): 67.
[6] Zhou L, Wu F, Zhang X, et al. International Journal of Food Properties, 2017, 20(Sup. 2): 1246.
[7] Wang Z, Han F, Sui X, et al. Journal of the Science of Food and Agriculture, 2016, 96(5): 1532.
[8] Gong J, Huang J, Xiao G, et al. Journal of the Institute of Brewing, 2017, 123(3): 417.
[9] Fan J, Li P, Li C, et al. Acta Universitatis Agriculturalis Boreali-Occidentalis, 2001, 29(1): 30.
[10] Liu F G, Sun C X, Yang W, et al. Rsc. Adv., 2015, 5(20): 15641.
[11] Wang Z, Li Y, Jiang L, et al. Journal of Chemistry, 2014, (2014): 1.
[12] Zhang H, Yao H Y, Chen F, et al. Food Chemistry, 2007, 105(1): 65.
[13] Tsuchiya K, Nishimura K, Iwahara M. Food Science and Technology Research, 2003, 9(15): 3226.
[14] Matsumoto T, Yamaura I, Funatsu M. Agricultural and Biological Chemistry, 1986, 50(6): 1413.
[15] Yang T, Peng H, Bauchan G R. Horticulture Research, 2014, (1): 14057.
[16] Inatomi K, Slaughter J C. Biochemical Journal, 1975, 147(3): 479.
[17] Wang L, Xu D X, Lv Y G, et al. Journal of the Science of Food and Agriculture, 2010, 90(6): 1027.
[18] Matsumoto T, Yamaura I, Funatsu M. Agricultural and Biological Chemistry, 1990, 54(11): 3001.
[19] Gut H, Dominici P, Pilati S, et al. Journal of Molecular Biology, 2009, 392(2): 334.
[20] Satyanarayan V, Nair P M. European Journal of Biochemistry, 1985, 150(1): 53.
[21] Hiraga K, Ueno Y, Oda K. Bioscience, Biotechnology, and Biochemistry, 2008, 72(5): 1299.
[22] Snedden W A, Arazi T, Fromm H, et al. Plant Physiology, 1995, 108(2): 543.
[23] Yang S Y, Lu Z X, Sun L J, et al. Food Science, 2007, (2): 49.
[24] Qing-Yun B. Journal of Anhui Agricultural Sciences, 2011, (3): 7.
[25] Mauerer A, Lee G. European Journal of Pharmaceutics and Biopharmaceutics, 2006, 62(2): 131.
[26] Chen C H, Wu S J, Martin D L. Archives of Biochemistry and Biophysics, 1998, 349(1): 175.
[27] Yuan T, Vogel H J. Journal of Biological Chemistry, 1998, 273(46): 30328.
[28] Yun S J, Oh S H. Molecules and Cells, 1998, 8(2): 125.
[29] Zhou L, Yang Y, Ren H, et al. Journal of Chemistry, 2016, 2016: 1.
[30] Li C E. Trends in Food Science & Technology, 1996, 11(7): 361.
[31] Lord R C, Yu N T. Journal of Molecular Biology, 1970, 50(2): 509.
[32] Herrero A M. Critical Reviews in Food Science and Nutrition, 2008, 48(6): 512.
[33] Vivian J T, Callis P R. Biophysical Journal, 2001, 80(5): 2093.
[34] O’Leary M H, Brummund W. Journal of Biological Chemistry, 1974, 249(12): 3737.
[35] Tramonti A, John R A, Bossa F, et al. European Journal of Biochemistry, 2002, 269(20): 4913. |
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