|
|
|
|
|
|
Study on the Interaction of Tetra-Brominated Diphenyl Ethers (BDE47) with Lysozyme |
YANG Lu-lu, YANG Wu, YI Zhong-sheng*, ZHAO Sai, DUAN Jia-xi |
Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China |
|
|
Abstract The binding of BDE47 to lysozyme was investigated by molecular modeling combined with three-dimensional (3D) fluorescence, circular dichroism (CD) techniques, fluorescence spectroscopy, and time-resolved fluorescence decay under simulative physiological conditions. The results indicated that the quenching reaction of BDE47 to lysozyme was observed, and the quenching mechanism was suggested as static quenching. Molecular docking showed that the amino acid residues, TRP62, TRP63, ARG61, ASN59, ALA107, ILE98 in lysozyme have interactions with BDE47. The hydrogen bonds were formed between the O atom of BDE47 and TRP62 with the distances of 2.2 Å. The 3D fluorescence experiments showed that the fluorescence intensity of lysozyme gradually decreased in the presence of BDE47 and a red shifted was observed, suggesting that the microenvironment around the TRP-residues of lysozyme has changed during the binding process. Furthermore, CD spectra implied that the interaction of BDE47 with lysozyme induced conformational change of lysozyme, and the content of α-helix structures in lysozyme decreased. The binding distance r between the donor (lysozyme) and acceptor (BDE47) calculated using Förster’s nonradiative energy transfer theory was 3.31 nm, indicating a high probability of energy transfer from lysozyme to BDE47. The thermodynamic parameters at different temperatures indicated that the hydrogen bonds and van der Waals forces played a predominant role in the spontaneous binding process. The results were consistent with the molecular docking and binding free energy analysis.
|
Received: 2016-11-11
Accepted: 2018-02-09
|
|
Corresponding Authors:
YI Zhong-sheng
E-mail: yzs@glut.edu.cn
|
|
[1] LIU Han-xia, ZHANG Qing-hua, JIANG Gui-bin(刘汉霞, 张庆华, 江桂斌). Progress in Chemistry(化学进展), 2005, 17(3): 554.
[2] Hu X N, Zhang J Q, Jiang Y S, et al. Toxicol. Vitro., 2014, 28(8): 1377.
[3] Meironyté G D, Bergman Å, Norén K. Arch. Environ. Contam. Toxicol., 2001, 40(4): 564.
[4] WAN Bin, GUO Liang-hong(万 斌, 郭良宏). Environmental Chemistry(环境化学), 2011, 30(1): 143.
[5] Sara J L, Barbara P M, James R O, et al. Chem. Res. Toxicol., 2009, 22: 1802.
[6] Wu D, Yan J, Tang P X, et al. Food Chem., 2015, 188: 370.
[7] Jeffrey S N, John J R, Bruce S Z. Cancer Res., 1981, 4: 1642.
[8] Roy A S, Ghosh P. J. Incl. Phenom. Macrocycl. Chem., 2016, 84: 21.
[9] Wang Q, Ma X L, He J W, et al. Spectrochim. Acta Pt. A-Molec. Biomolec. Spectr., 2016, 153: 612.
[10] Paramaguru G, Kathiravan A, Selvaraj S, et al. J. Hazard. Mater., 2010, 175: 985.
[11] Peng W, Ding F, Peng Y K, et al. J. Agric. Food Chem., 2013, 61(50): 12415.
[12] Chakraborti S, Chatterjee T, Joshi P, et al. Langmuir, 2010, 26(5): 3506.
[13] Revathi R, Rameshkumar A, Sivasudha T. Spectrochim. Acta Pt. A-Molec. Biomolec. Spectr., 2016, 152: 192.
[14] HU Guo-dong, ZHANG Shao-long, ZHANG Qing-gang(扈国栋, 张少龙, 张庆刚). Acta Chim. Sinica(化学学报), 2009, 67(9): 1019.
[15] Zhang L, Sun Y. Langmuir, 2014, 30(16): 4725.
[16] HU Jian-ping, SUN Ting-guang, CHEN Wei-zu, et al(胡建平, 孙庭广, 陈慰祖, 等). Acta Chim. Sinica(化学学报), 2006, 64(20): 2079.
[17] Yue Y, Liu J, Yao M, et al. Spectrochim. Acta Pt. A-Molec. Biomolec. Spectr., 2012, 96: 316.
[18] GUO Qing-lian, PAN Ling-li, YANG Li-yun, et al(郭清莲, 潘凌立, 杨立云, 等). Acta Phys.-Chim. Sin.(物理化学学报), 2016, 32(1): 274.
[19] Greenfield N, Fasman G D. Biochemistry, 1969, 8(10): 4108.
[20] Ma F, Huang H Y, Zhou L, et al. Spectrochim. Acta Pt. A-Molec. Biomolec. Spectr., 2012, 97: 1159.
[21] Ross P D, Subramanian S. Biochemistry, 1981, 20: 3096. |
[1] |
ZHENG Hong-quan, DAI Jing-min*. Research Development of the Application of Photoacoustic Spectroscopy in Measurement of Trace Gas Concentration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 1-14. |
[2] |
CHENG Jia-wei1, 2,LIU Xin-xing1, 2*,ZHANG Juan1, 2. Application of Infrared Spectroscopy in Exploration of Mineral Deposits: A Review[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 15-21. |
[3] |
FAN Ping-ping,LI Xue-ying,QIU Hui-min,HOU Guang-li,LIU Yan*. Spectral Analysis of Organic Carbon in Sediments of the Yellow Sea and Bohai Sea by Different Spectrometers[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 52-55. |
[4] |
LI Jie, ZHOU Qu*, JIA Lu-fen, CUI Xiao-sen. Comparative Study on Detection Methods of Furfural in Transformer Oil Based on IR and Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 125-133. |
[5] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
[6] |
XU Tian1, 2, LI Jing1, 2, LIU Zhen-hua1, 2*. Remote Sensing Inversion of Soil Manganese in Nanchuan District, Chongqing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 69-75. |
[7] |
WANG Fang-yuan1, 2, HAN Sen1, 2, YE Song1, 2, YIN Shan1, 2, LI Shu1, 2, WANG Xin-qiang1, 2*. A DFT Method to Study the Structure and Raman Spectra of Lignin
Monomer and Dimer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 76-81. |
[8] |
YANG Cheng-en1, 2, LI Meng3, LU Qiu-yu2, WANG Jin-ling4, LI Yu-ting2*, SU Ling1*. Fast Prediction of Flavone and Polysaccharide Contents in
Aronia Melanocarpa by FTIR and ELM[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 62-68. |
[9] |
LIU Zhen1*, LIU Li2*, FAN Shuo2, ZHAO An-ran2, LIU Si-lu2. Training Sample Selection for Spectral Reconstruction Based on Improved K-Means Clustering[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 29-35. |
[10] |
YANG Chao-pu1, 2, FANG Wen-qing3*, WU Qing-feng3, LI Chun1, LI Xiao-long1. Study on Changes of Blue Light Hazard and Circadian Effect of AMOLED With Age Based on Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 36-43. |
[11] |
GAO Feng1, 2, XING Ya-ge3, 4, LUO Hua-ping1, 2, ZHANG Yuan-hua3, 4, GUO Ling3, 4*. Nondestructive Identification of Apricot Varieties Based on Visible/Near Infrared Spectroscopy and Chemometrics Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 44-51. |
[12] |
ZHENG Pei-chao, YIN Yi-tong, WANG Jin-mei*, ZHOU Chun-yan, ZHANG Li, ZENG Jin-rui, LÜ Qiang. Study on the Method of Detecting Phosphate Ions in Water Based on
Ultraviolet Absorption Spectrum Combined With SPA-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 82-87. |
[13] |
XU Qiu-yi1, 3, 4, ZHU Wen-yue3, 4, CHEN Jie2, 3, 4, LIU Qiang3, 4 *, ZHENG Jian-jie3, 4, YANG Tao2, 3, 4, YANG Teng-fei2, 3, 4. Calibration Method of Aerosol Absorption Coefficient Based on
Photoacoustic Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 88-94. |
[14] |
LI Xin-ting, ZHANG Feng, FENG Jie*. Convolutional Neural Network Combined With Improved Spectral
Processing Method for Potato Disease Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 215-224. |
[15] |
XING Hai-bo1, ZHENG Bo-wen1, LI Xin-yue1, HUANG Bo-tao2, XIANG Xiao2, HU Xiao-jun1*. Colorimetric and SERS Dual-Channel Sensing Detection of Pyrene in
Water[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 95-102. |
|
|
|
|