| 光谱学与光谱分析 |
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| Study on Content Determination of Lead and Arsenic in Four Traditional Tibetan Medicine Prescription Preparations by Wet Digestion Flow Injection-Hydride Generation-Atomic Absorption Spectrometry |
| ZHENG Zhi-yuan1, 2, 3, DU Yu-zhi1, 2, ZHANG Ming1, 2, 3, YU Ming-jie1, 2, 3, LI Cen1, 2, 3, YANG Hong-xia1, 2, ZHAO Jing1, 2, 3, XIA Zheng-hua1, 2, 3, WEI Li-xin1, 2* |
1. Northwestern Institute of Plateau Biology，Chinese Academy of Sciences，Xining 810001，China
2. Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation，Xining 810001，China
3. University of Chinese Academy of Sciences，Beijing 100049，China |
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Abstract Four common traditional tibetan medicine prescription preparations “Anzhijinghuasan，Dangzuo，Renqingchangjue and Rannasangpei” in tibetan areas were selected as study objects in the present study. The purpose was to try to establish a kind of wet digestion and flow injection-hydride generation-atomic absorption spectrometry (FI-HAAS) associated analysis method for the content determinations of lead and arsenic in traditional tibetan medicine under optimized digestion and measurement conditions and determine their contents accurately. Under these optimum operating conditions，experimental results were as follows. The detection limits for lead and arsenic were 0.067 and 0.012 μg·mL-1 respectively. The quantification limits for lead and arsenic were 0.22 and 0.041 μg·mL-1 respectively. The linear ranges for lead and arsenic were 25～1 600 ng·mL-1 (r=0.999 5) and 12.5～800 ng·mL-1(r=0.999 4) respectively. The degrees of precision(RSD) for lead and arsenic were 2.0% and 3.2% respectively. The recovery rates for lead and arsenic were 98.00%～99.98% and 96.67%～99.87% respectively. The content determination results of lead and arsenic in four traditional tibetan medicine prescription preparations were as follows. The contents of lead and arsenic in Anzhijinghuasan are 0.63～0.67 μg·g-1 and 0.32～0.33 μg·g-1in Anzhijinghuasan，42.92～43.36 μg·g-1 and 24.67～25.87 μg·g-1 in Dangzuo，1 611.39～1 631.36 μg·g-1 and 926.76～956.52 μg·g-1 in Renqing Changjue，and 1 102.28～1 119.27 μg·g-1 and 509.96～516.87 μg·g-1 in Rannasangpei，respectively. This study established a method for content determination of lead and arsenic in traditional tibetan medicine，and determined the content levels of lead and arsenic in four tibetan medicine prescription preparations accurately. In addition，these results also provide the basis for the safe and effective use of those medicines in clinic.
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Received: 2014-04-07
Accepted: 2014-07-29
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Corresponding Authors:
WEI Li-xin
E-mail: lxwei@nwipb.cas.cn
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