光谱学与光谱分析 |
|
|
|
|
|
Determination of Recombinant Hirudin in Urine of Rat by Spectrophotometric Method |
JIANG Su-yun1,2, JIAO Jian2, LU Jun2, XU Yong-ping1*, DOU Gui-fang3 |
1. Dalian University of Technology, Dalian 116023, China 2. Dalian Institute for Food and Drug Control, Dalian 116021, China 3. Beijing Military Academy of Medical Sciences, Beijing 100039, China |
|
|
Abstract To develop a spectrophotometric method for determining the concentration of recombinant hirudin (rH) in urine of rats. rH concentration was determined based on the rH inhibility to thrombin which hydrolyzed the Chromozym TH TH chromogenic substrate to form the specific pNA absorbed at 405nm. The standard rH in rat urine was determined by the spectrophotometric method at concentration of 6.25 to 75 ng·mL-1 with day and intra-day RSD <10%, method recoveries of >95% and the dilution recoveries of >93%. The rH samples of rat urines which iv dose of 0.5, 1.0, and 2.0 mg·kg-1 were collected and analyzed by the CSA method. Their cumulative excretion rH at 0~12 hr were (116.850±57.160),(235.544±39.375) and (474.986±85.426) μg·kg-1. The calculated cumulative excretion rate of three doses is about 23% which indicates that the rH was eliminated in the way of a linear kinetics in rats. The rH content in rat urine could be measured by the spectrophotometric method accurately, reliably and sensitively for the rH urinary excretion dynamics study.
|
Received: 2013-01-22
Accepted: 2013-03-25
|
|
Corresponding Authors:
XU Yong-ping
E-mail: sembiao@126.com
|
|
[1] Markwardt F. Methods Enzymology, 1970, 69: 924. [2] Markwardt F. Thrombin Res., 1994, 74(1): 1. [3] Lü Li,LI Xin-yan,HAN Guo-zhu(吕 莉,李欣燕,韩国柱). Chinese Tradition Herba Drugs(中草药),2002,33(6): 520. [4] REN Kai-huan,HAN Guo-zhu,LI Wei-ping(任开环,韩国柱,李卫平). Herald of Medicine(医药导报),2005,24(5):369. [5] HUANG Yu-rong,LI Quan-sheng,LIU Chang-xiao(黄玉荣,李全胜,刘昌孝). Chinese Traditional and Herbal Drugs(中草药),2005,36(9):1352. [6] JIANG Su-yun,LI Guo-xiang,HAN Guo-zhu(姜素云, 李国香, 韩国柱). Chinese Pharmacentional Journal(中国药学杂志), 2003,3(2):129. [7] JIANG Su-yun,LU Jun,HAN Guo-zhu(姜素云, 鲁 军, 韩国柱). Chinese Journal of Pharmacology and Toxicology(中国药理学与毒理学杂志),2007,21(4):322. [8] REN Hong-can, RUI Ming, HAN Guo-zhu, et al(任洪灿, 芮 明, 韩国柱,等). Chinese Journal of Anaytical Chemistry(分析化学),2006,34(4):547. [9] YAN Yu-hui,WANG Yi-nan,HAN Guo-zhu(阎宇辉, 王一楠, 韩国柱). Chinese Journal of Analytical Chmistry(分析化学),2007,35(5):691. [10] ZHANG Ting-ting,JIANG Su-yun,JIAO Jian,et al(张婷婷,姜素云,矫 建,等). Chinese Journal New Drugs (中国新药杂志), 2011,20(4):372. [11] Bichler J, Fichtl B, Siebeck M, et al. Arzneittelforschung,1988,38: 704. [12] Markwardt F, Hauptmann J, Nowak G, et al. Thromb Heamost,1982,47: 226. |
[1] |
ZHANG Rong1, 2, DUAN Ning1, 3, JIANG Lin-hua1, 3*, XU Fu-yuan3, JIN Wei3, LI Jian-hui1. Study on Optical Path Optimization for Direct Determination of
Spectrophotometry of High Concentration Hexavalent Chromium
Solution by Ultraviolet Visible Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1829-1837. |
[2] |
LI Yun-qi1, ZHANG Ning2, CHEN De-fu3, QIU Hai-xia1, ZENG Jing1, WANG Na5*, GU Ying1, 3, 4*. Beneficial Effects of Short-Wavelength Narrow Band Ultraviolet Irradiation Delivered Using an LED Device on Bone Metabolism in Rats[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2649-2656. |
[3] |
YAO Shan1, ZHANG Xuan-ling1, CAI Yu-xin1, HE Lian-qiong1, LI Jia-tong1, WANG Xiao-long1, LIU Ying1, 2*. Study on Distribution Characteristics of Different Nitrogen and
Phosphorus Fractions by Spectrophotometry in Baiyangdian
Lake and Source Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1306-1312. |
[4] |
LIU Qin-rong1, DU Zi-wei1, LI Jia-zhen1, WANG Yi-shuo1, 3*, GU Xuan2, CUI Xiu-mei2. Analysis and Evaluation of Inorganic Elements in Salvia miltiorrhiza and Rhizosphere Soils From Different Areas[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(11): 3618-3624. |
[5] |
LI Zhen-xia1, SHEN Huan-huan1, GAO Miao-miao1, QI Jiao1, LI Qing-yan2*. Analysis of Lycopene in Different Solvents by Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(04): 1114-1117. |
[6] |
YANG Ze-ming1, 2, LI Cai1*, LU Gui-xin1, CAO Wen-xi1. A Study of Fast Detection of Nitrite in Seawater Based on Sequential Injection Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 589-595. |
[7] |
LINGYUN Xia-fei1, 2, GAO Chao1, 2, FAN Jing-shuang1, 2, Lü Hai-xia1, 2*, YU Yan1, 2. Synthesis of Hyperbranched Polyamidoamine (PAMAM) Grafted Chitosan and Its Adsorption for Heavy Metal and Dyes Studied with Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3583-3587. |
[8] |
CHEN Jing1, 2, 4, PENG Jiao-yu1, 2, BIAN Shao-ju1, 3, GAO Dan-dan1, 3, DONG Ya-ping1, 2*, LI Wu1, 3. Optimization of Determination of CO2-3 and HCO-3 in Boron-Containing Brine[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2194-2199. |
[9] |
LI Yun1,2,3, ZHANG Ji1,2, LIU Fei4, XU Fu-rong3, WANG Yuan-zhong1,2*, ZHANG Jin-yu1,2,3*. Prediction of Total Polysaccharides Content in P. notoginseng Using FTIR Combined with SVR[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1696-1701. |
[10] |
WU Xin1,2, LI Guang-lin1*, WEN Zhi-yu3. Study and Determination the Concentration of CNO-Ion of the QPQ with the Sequential Injection Spectrophotometric Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1824-1828. |
[11] |
CAO Xuan1, 2,ZHANG Shu-wei1, 2,CHU Dong-zhi1, 2,WU Ning1, 2,MA Hai-kuan1, 2,MA Ran1, 2. Development of Lab-on-Chip Spectrophotometric Silicate Sensor in situ Analysis of Seawater[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 895-900. |
[12] |
HE Qiu-ju1,2, WANG Li-qin1*, ZHANG Ya-xu1. Study of Mechanism of Aluminum Sizing Precipitant on Xuan Paper Based on Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 418-423. |
[13] |
ZHU Hong-qiu, GONG Juan, LI Yong-gang*, CHEN Jun-ming. A Spectrophotometric Detecting Method of Trace Cobalt under High Concentrated Zinc Solution[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3882-3888. |
[14] |
GONG Hong-ling1, LIU Yan-hui2*, TANG Yan-lin2*, HU Lin2. Effects of Temperature on DNA Condensation Detected with Temperature-Changed Ultraviolet Spectrum Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1831-1837. |
[15] |
LIU Qian3, CHEN Wen-juan1,2, JING Bo1,2, ZHANG Jian1,2, YANG Meng-ying3, CHEN Wu3, YIN Xian-qing1,3*. Spectrum Analysis of Trace Hydrogen Peroxide in Electrochemical Process[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1656-1660. |
|
|
|
|