| 光谱学与光谱分析 |
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| Feasibility Reaserch on Using Raman Spectroscopy with PLS for the Quantitative Detection of Cholesterol Content in Serum |
| DONG Hai-sheng1, ZHANG Li-fen2, ZHONG Yue2, HUANG Jian-ying1, CHEN Bin1* |
1. State Key Lab of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094，China
2. Out-Patient Department of Beijing Aerospace City, Beijing 100094，China |
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Abstract Taking the serum obtained from daily clinical inspection as study object, the Raman spectra were collected with the range of 250 to 2 400 cm-1. Taking the serum cholesterol content determined by traditional clinical biochemical method as contrast, together with combination of PLS, we established a Raman spectroscopy quantitative models for cholesterol content in serum. The experimental results show that Raman spectroscopy displacement could reflect certain chemical group in organic compound. The correction model of cholesterol content in serum was established with the coefficient R=0.909 6, cross-validation calibration standard deviation (RMSECV) of 0.24, standard error of prediction of test set (RMSEP) was 0.69, and coefficient (R) is 0.926 2. The results showed that application of Raman spectroscopy combined with chemometrics in the rapid noninvasive detection of serum cholesterol content is feasible.
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Received: 2012-10-17
Accepted: 2013-01-15
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Corresponding Authors:
CHEN Bin
E-mail: chenb12@yahoo.com.cn
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